Entry Name 8-demethyl-8-aminoriboflavin-5'-phosphate synthase;
Class Transferases;BRITE hierarchy
Sysname L-glutamate:FMN aminotransferase (oxidizing, decarboxylating)
Reaction(IUBMB) L-glutamate + FMN + O2 + H2O + 3 acceptor = 2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + 3 reduced acceptor (overall reaction) [RN:
R11960 ];
(1a) FMN + O2 = 8-demethyl-8-formylriboflavin 5'-phosphate + H2O [RN:
R11961 ];
(1b) 8-demethyl-8-formylriboflavin 5'-phosphate + H2O + acceptor = 8-carboxy-8-demethylriboflavin 5'-phosphate + reduced acceptor [RN:
R11962 ];
(1c) L-glutamate + 8-carboxy-8-demethylriboflavin 5'-phosphate + H2O + 2 acceptor = 2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + 2 reduced acceptor [RN:
R11963 ]
Reaction(KEGG) Substrate L-glutamate [CPD:
C00025 ];
FMN [CPD:
C00061 ];
O2 [CPD:
C00007 ];
H2O [CPD:
C00001 ];
acceptor [CPD:
C00028 ];
8-demethyl-8-formylriboflavin 5'-phosphate [CPD:
C21803 ];
8-carboxy-8-demethylriboflavin 5'-phosphate [CPD:
C21804 ]
Product 2-oxoglutarate [CPD:
C00026 ];
8-amino-8-demethylriboflavin 5'-phosphate [CPD:
C21805 ];
CO2 [CPD:
C00011 ];
reduced acceptor [CPD:
C00030 ];
8-demethyl-8-formylriboflavin 5'-phosphate [CPD:
C21803 ];
H2O [CPD:
C00001 ];
8-carboxy-8-demethylriboflavin 5'-phosphate [CPD:
C21804 ]
Comment The enzyme, characterized from the bacterium Streptomyces davawensis, has the activities of an oxidoreductase, a decarboxylase, and an aminotransferase. Its combined actions result in the replacement of a methyl substituent of one of the aromatic rings of FMN by an amino group, a step in the biosynthetic pathway of roseoflavin. The reaction requires thiamine for completion.
History EC 2.6.1.114 created 2018
Pathway ec00998 Biosynthesis of various antibiotics
ec01110 Biosynthesis of secondary metabolites
Orthology K22598 8-demethyl-8-aminoriboflavin-5'-phosphate synthase
Genes Taxonomy Reference Authors Schwarz J, Konjik V, Jankowitsch F, Sandhoff R, Mack M
Title Identification of the Key Enzyme of Roseoflavin Biosynthesis.
Journal Sequence Reference Authors Jhulki I, Chanani PK, Abdelwahed SH, Begley TP
Title A Remarkable Oxidative Cascade That Replaces the Riboflavin C8 Methyl with an Amino Group during Roseoflavin Biosynthesis.
Journal Sequence Reference Authors Konjik V, Brunle S, Demmer U, Vanselow A, Sandhoff R, Ermler U, Mack M
Title The Crystal Structure of RosB: Insights into the Reaction Mechanism of the First Member of a Family of Flavodoxin-like Enzymes.
Journal Sequence Other DBs ExPASy - ENZYME nomenclature database: 2.6.1.114
