Entry
Name
2'-deamino-2'-hydroxyneamine transaminase;
kacL (gene name)
Class
Transferases;
Transferring nitrogenous groups;
Transaminases
BRITE hierarchy
Sysname
2'-deamino-2'-hydroxyneamine:2-oxoglutarate aminotransferase
Reaction(IUBMB)
2'-deamino-2'-hydroxyneamine + 2-oxoglutarate = 2'-deamino-2'-hydroxy-6'-dehydroparomamine + L-glutamate [RN:
R10094 ]
Reaction(KEGG)
Substrate
2'-deamino-2'-hydroxyneamine [CPD:
C20350 ];
2-oxoglutarate [CPD:
C00026 ]
Product
2'-deamino-2'-hydroxy-6'-dehydroparomamine [CPD:
C20351 ];
L-glutamate [CPD:
C00025 ]
Comment
The reaction occurs in vivo in the opposite direction. Involved in the biosynthetic pathway of kanamycin A and kanamycin D. The enzyme, characterized from the bacterium Streptomyces kanamyceticus, can also catalyse EC
2.6.1.93 , neamine transaminase.
History
EC 2.6.1.94 created 2012
Pathway
ec00524 Neomycin, kanamycin and gentamicin biosynthesis
ec01110 Biosynthesis of secondary metabolites
Orthology
K20568 neamine transaminase / 2'-deamino-2'-hydroxyneamine transaminase
Reference
Authors
Park JW, Park SR, Nepal KK, Han AR, Ban YH, Yoo YJ, Kim EJ, Kim EM, Kim D, Sohng JK, Yoon YJ
Title
Discovery of parallel pathways of kanamycin biosynthesis allows antibiotic manipulation.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.6.1.94
ExPASy - ENZYME nomenclature database: 2.6.1.94