KEGG   ENZYME: 2.7.1.228
Entry
EC 2.7.1.228                Enzyme                                 
Name
mannosyl-inositol-phosphoceramide inositolphosphotransferase;
IPT1 (gene name)
Class
Transferases;
Transferring phosphorus-containing groups;
Phosphotransferases with an alcohol group as acceptor
Sysname
1-phosphatidyl-1D-myo-inositol:(4R)-4-hydroxy-N-[(2R)-2-hydroxy-very-long-chain-acyl]-1-O-{[6-O-(alpha-D-mannosyl)-1D-myo-inositol-1-O-yl]hydroxyphosphoryl}sphinganine phosphoinositoltransferase
Reaction(IUBMB)
1-phosphatidyl-1D-myo-inositol + a (4R)-4-hydroxy-N-[(2R)-2-hydroxy-very-long-chain-acyl]-1-O-{[6-O-(alpha-D-mannosyl)-1D-myo-inositol-1-O-yl]hydroxyphosphoryl}sphinganine = 1,2-diacyl-sn-glycerol + a (4R)-4-hydroxy-N-[(2R)-2-hydroxy-very-long-chain-acyl]-1-O-[(6-O-{6-O-[(1D-myo-inositol-1-O-yl)hydroxyphosphoryl]-alpha-D-mannosyl}-1D-myo-inositol-1-O-yl)hydroxyphosphoryl]sphinganine
Substrate
1-phosphatidyl-1D-myo-inositol [CPD:C01194];
(4R)-4-hydroxy-N-[(2R)-2-hydroxy-very-long-chain-acyl]-1-O-{[6-O-(alpha-D-mannosyl)-1D-myo-inositol-1-O-yl]hydroxyphosphoryl}sphinganine
Product
1,2-diacyl-sn-glycerol [CPD:C00641];
(4R)-4-hydroxy-N-[(2R)-2-hydroxy-very-long-chain-acyl]-1-O-[(6-O-{6-O-[(1D-myo-inositol-1-O-yl)hydroxyphosphoryl]-alpha-D-mannosyl}-1D-myo-inositol-1-O-yl)hydroxyphosphoryl]sphinganine
Comment
This enzyme catalyses the ultimate reaction in the yeast sphingolipid biosynthesis pathway. It transfers a second phosphoinositol group to mannosyl-inositol-phospho-alpha-hydroxyphytoceramide (MIPC), forming the final and most abundant yeast sphingolipid, mannosyl-diphosphoinositol-ceramide (MIP2C).
History
EC 2.7.1.228 created 2019
Orthology
K22725  mannosyl-inositol-phosphoceramide inositolphosphotransferase
Genes
SCE: YDR072C(IPT1)
SEUB: DI49_0268
SPAO: SPAR_D02880
ERC: Ecym_5553
KLA: KLLA0_E10187g
KMX: KLMA_20024(IPT1)
LTH: KLTH0H07590g
VPO: Kpol_1032p31
ZRO: ZYRO0G14718g
CGR: CAGL0G05313g
NCS: NCAS_0B04230(NCAS0B04230)
NDI: NDAI_0A03650(NDAI0A03650)
TPF: TPHA_0A02150(TPHA0A02150)
TBL: TBLA_0F03590(TBLA0F03590)
TGB: HG536_0D02150
TDL: TDEL_0B04170(TDEL0B04170)
KAF: KAFR_0C02620(KAFR0C02620)
KNG: KNAG_0D01900(KNAG0D01900)
PPA: PAS_chr3_0735
DHA: DEHA2F25520g
PIC: PICST_49558 PICST_61648 PICST_63545 PICST_78790
PGU: PGUG_03091 PGUG_05895
SPAA: SPAPADRAFT_58326 SPAPADRAFT_58414
LEL: PVL30_000794
CAL: CAALFM_C109120WA(IPT1)
CTP: CTRG_04128
COT: CORT_0A07460 CORT_0A08040
CDU: CD36_08590
CTEN: CANTEDRAFT_124983
CLU: CLUG_04090
CLUS: A9F13_01g00176
CAUR: CJI96_0002079 CJI96_0002609
BNN: FOA43_001983
SLUD: SCDLUD_001622
SSL: SS1G_02388
BFU: BCIN_13g02520(Bcipt1)
MBE: MBM_07403
PSCO: LY89DRAFT_727904
GLZ: GLAREA_01667
 » show all
Reference
1  [PMID:9368028]
  Authors
Dickson RC, Nagiec EE, Wells GB, Nagiec MM, Lester RL
  Title
Synthesis of mannose-(inositol-P)2-ceramide, the major sphingolipid in Saccharomyces cerevisiae, requires the IPT1 (YDR072c) gene.
  Journal
J Biol Chem 272:29620-5 (1997)
DOI:10.1074/jbc.272.47.29620
  Sequence
[sce:YDR072C]
Other DBs
ExplorEnz - The Enzyme Database: 2.7.1.228
IUBMB Enzyme Nomenclature: 2.7.1.228
ExPASy - ENZYME nomenclature database: 2.7.1.228
BRENDA, the Enzyme Database: 2.7.1.228

  All links  
Gene (47)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (46)   
Protein sequence (3)   
   UniProt (1)   
   SWISS-PROT (1)   
   RefSeq(pep) (1)   
DNA sequence (3)   
   RefSeq(nuc) (2)   
   EMBL (1)   
Protein domain (2)   
   InterPro (2)   
All databases (55)   

Download RDF
DBGET integrated database retrieval system