KEGG   ENZYME: 2.7.1.95
Entry
EC 2.7.1.95                 Enzyme                                 
Name
kanamycin kinase;
neomycin-kanamycin phosphotransferase
Class
Transferases;
Transferring phosphorus-containing groups;
Phosphotransferases with an alcohol group as acceptor
Sysname
ATP:kanamycin 3'-O-phosphotransferase
Reaction(IUBMB)
ATP + kanamycin = ADP + kanamycin 3'-phosphate
Reaction(KEGG)
(other) R01888
Substrate
ATP [CPD:C00002];
kanamycin [CPD:C00304]
Product
ADP [CPD:C00008];
kanamycin 3'-phosphate [CPD:C03281]
Comment
Also acts on the antibiotics neomycin, paromomycin, neamine, paromamine, vistamycin and gentamicin A. An enzyme from Pseudomonas aeruginosa also acts on butirosin.
History
EC 2.7.1.95 created 1976
Orthology
K00897  kanamycin kinase
K19272  aminoglycoside 3'-phosphotransferase I
K19274  aminoglycoside 3'-phosphotransferase VI
K19299  aminoglycoside 3'-phosphotransferase III
K19300  aminoglycoside 3'-phosphotransferase II
K19315  aminoglycoside 3'-phosphotransferase XV
K19543  aminoglycoside 3'-phosphotransferase
Genes
MPHA: 105840509
EHI: EHI_117050
EBW: BWG_3698
EOI: ECO111_p1-120
EOJ: ECO26_p2-04
ECM: EcSMS35_A0159
SEC: SCH_026(aph)
ECLE: ECNIH2_23600 ECNIH2_26045
ECLY: LI62_013010 LI62_11650
KPN: KPN_pKPN5p08194(aphA)
KPZ: KPNIH27_25655
KPG: KPNIH32_28420
KPC: KPNIH10_26765
KPB: FH42_25875
KPNE: KU54_00025
KPNU: LI86_26895
KOX: KOX_20540
KOE: A225_2977
KOY: J415_17065
KOM: HR38_18875
KOK: KONIH1_14625 KONIH1_30465
KOC: AB185_22765
CFD: CFNIH1_24930
CWE: CO701_09595
CPAR: CUC49_08460
CITZ: E4Z61_00180
CIX: M4I31_16720
KIE: NCTC12125_04789(neo)
KIN: AB182_03320
SMAF: D781_0520
SRZ: AXX16_0005
SERM: CLM71_02525
SRHZ: FO014_07330
PANP: PSNIH2_20340
PCOL: F1325_16150(aph(3')-I)
PCIB: F9282_16450(aph(3')-VI)
PSTA: BGK56_21235
MWI: MNY66_17570
PMP: Pmu_02740(aphA1)
MHX: MHH_c22550(aphA1)
AIO: EXH44_00925
GAN: UMN179_01162
BTO: WQG_1070
BTRH: F543_22790
BTRA: F544_3130
SML: Smlt2120
SMT: Smal_1717
BUJ: BurJV3_1756
SMZ: SMD_1909
STEN: CCR98_09260
STEM: CLM74_09685
STES: MG068_09120(aph(3')-II)
SINC: DAIF1_18880(neo)
SPAQ: STNY_R18290(aph(3')-II)
SGEN: RKE57_09100
LMB: C9I47_1673
LUE: DCD74_02565(aph(3')-XV)
DKO: I596_3655
PAE: PA4119(aph)
PAEV: N297_4251(neo)
PAEI: N296_4251(neo)
PAU: PA14_10670(aph)
PAP: PSPA7_0973(aph) PSPA7_3721
PAG: PLES_08521(aph)
PNC: NCGM2_5320(aph)
PAEB: NCGM1900_0841(aph)
PSG: G655_04105
PRP: M062_21725
PAEP: PA1S_04225
PAER: PA1R_gp2010
PAEM: U769_04250
PAEL: T223_04160
PAES: SCV20265_0848
PAEU: BN889_04573(aph)
PAEG: AI22_29390
PAEC: M802_4249(neo)
PAEO: M801_4117(neo)
PPAA: B7D75_04095(aph(3')-II)
PPUH: B479_26894
PALD: LU682_024260
PFB: VO64_1917
PTV: AA957_19685
PFW: PF1751_v1c45260
PFF: PFLUOLIPICF713405
PSIL: PMA3_18220
PYM: AK972_1650
PUM: HGP31_03940
PGY: AWU82_21750(aph(3')-I)
PSHH: HU773_006205
PMUY: KSS95_22410
PJU: L1P09_08635 L1P09_25445(aph(3')-XV)
PPHN: HU825_15885(aph(3')-II)
PPAE: LDL65_05770
PSTU: UIB01_10035
PCHL: LLJ08_09385
SDEG: GOM96_02975(aph(3')-II)
DCE: O6P33_04430(aph(3')-XV) O6P33_04935
ABY: ABAYE3578
ABN: AB57_0288(aphA1b)
ABZ: ABZJ_05085
ABD: ABTW07_3876(aphA1b) ABTW07_3891(aphA1b)
ABJ: BJAB07104_p0106
ABAB: BJAB0715_p0002
ABAJ: BJAB0868_p0097
ABAZ: AGW27715.1
ABW: BL01_19125
ANO: RR32_19120
AJO: RZ95_17955
ASJ: AsACE_p200011
ADV: DJ533_01225 DJ533_07430
ACUM: C9E88_015545(aph(3')-VI)
AGU: AS4_23160
ACHI: CDG60_00305(aph(3')-VI) CDG60_00550
ATG: J4G44_12780
AVZ: HWI77_18555(aph(3')-XV)
APOT: MZO21_14195
MBL: AAX09_10060
SHEJ: MZ182_20690
PLZ: S4054249_10315
SAGA: M5M_18870
AEA: C2U39_23255(aph(3')-II)
AES: C2U30_02870(aph(3')-I)
SOK: D0B54_04480
NBL: GJV52_09465(aph(3')-IIIa)
CHIZ: HQ393_17285
CHIC: N8I74_12140
REH: H16_B1333(h16_B1333)
CNAN: A2G96_18890
BUR: Bcep18194_B2197
BCJ: BCAM0928(neo)
BCEO: I35_4848(neo)
BCED: DM42_4158(neo)
BCON: NL30_36050
BSTL: BBJ41_32400
BARI: NLX30_33815
PHS: C2L64_52670(aph(3')-I)
BTRM: SAMEA390648702979(neo)
AIS: BUW96_05100
ACHB: DVB37_20695
ADY: HLG70_04670
AALN: Q3V95_09670
AFA: UZ73_08990
NARM: N7E01_00080
DLA: I6G47_08505
MIU: ABE85_18090
JAZ: YQ44_09405
JAL: BZG29_20520
JSV: CNX70_20685
JAS: FJQ89_10355
JLV: G3257_21175(aph(3')-I)
JAH: JAB4_041730(neo)
MASY: DPH57_00520
MFY: HH212_05875
MASZ: C9I28_21630
MFLA: GO485_08340(aph(3')-II)
MPLI: E1742_08700
TCT: PX653_01165
DUG: HH213_14175(aph(3')-I)
TMJ: P0M04_06615
EHB: E7V67_019385
ETB: N7L95_04165
MLO: mlr2696
MESP: C1M53_20070
AAK: AA2016_2415
AMIH: CO731_02415(neo)
AMIS: Amn_40260
ANJ: AMD1_2311(neo)
SME: SMc03094
SMK: Sinme_3053
SMQ: SinmeB_2827
SMX: SM11_chr3177
SMI: BN406_02856(neo)
SMEG: C770_GR4Chr3137
SMEL: SM2011_c03094
SMER: DU99_16540
ENU: PYH37_005548
EGI: PZN02_001533
SKM: PZL22_003322
STEG: QA637_15250
EAH: FA04_16285
ECAA: J3R84_16250
ENP: JVX98_24050
RPUS: CFBP5875_03820
RET: RHE_CH02947
REC: RHECIAT_CH0003111
REL: REMIM1_CH02987
REP: IE4803_CH03173
REI: IE4771_CH03209
RLE: RL3397(neo)
RLT: Rleg2_2693
RLG: Rleg_2951
RLB: RLEG3_25360
RLU: RLEG12_24660
RTR: RTCIAT899_CH12390
RGA: RGR602_CH01541
RHN: AMJ98_CH03107
RPHA: AMC79_CH03109
RHX: AMK02_CH03039
RHK: Kim5_CH03209
REZ: AMJ99_CH03106
RHR: CKA34_15270
RAD: CO657_13770
RII: FFM53_020085
RHID: FFM81_006395
RBQ: J2J99_15455
RLN: J0663_02250
RRG: J3P73_17515
RAW: NE851_26470
RSUL: N2599_10280
RLW: RlegWSM1455_14850
RCT: PYR68_13135
ARA: Arad_3116
RBW: RLCC275e_15295
RIF: U5G49_003061
NGL: RG1141_CH16900
OIN: IAR37_12815
OAN: Oant_1320
OAH: DR92_883(neo)
OPS: A8A54_08305
BPSN: NIK97_06495(aph(3')-IId)
OCH: CES85_1994
OCR: HGK82_09445
OCL: GTN27_09755(aph(3'))
BRK: CWS35_05175
RPT: Rpal_0195
LNE: FZC33_33600
CHEL: AL346_10300
CDQ: BOQ54_16880
DEI: C4375_17355
DSAL: K1X15_08780
DEVO: H4N61_08845
DNP: N8A98_18860
DOY: JI749_12310
DRH: JI748_05895
MAAD: AZF01_20235
PSF: PSE_4262(neo)
PPHR: APZ00_03710
PSTG: E8M01_04605 E8M01_04630
CFH: C1707_08575
BND: KWG56_14220(aph(3')-XV)
NOR: FA702_16840(aph(3')-I)
SPHF: DM480_00550 DM480_17500(aph(3')-I)
SECH: B18_03710
SCHY: GVO57_08575(aph(3'))
SBIN: SBA_ch1_03620
SMOR: LHA26_07850
SRAD: LLW23_03260
SNAP: PQ455_10715
PHZ: CHX26_14640
AZZ: DEW08_09705
RCE: RC1_0237(butA)
CJEN: N755_01768(aphA)
CCC: G157_08935
CCY: YSS_10115
MYX: QEG98_28980
GCS: MUN88_08790
GSM: MUN87_10085
MEKU: HUW50_16765
BOU: I5818_13625 I5818_13630
SAX: pUSA300HOUMR0018
SUZ: MS7_0063(aphA)
SUW: SATW20_20760(aphA)
SAUG: SA268_2516
SAUZ: SAZ172_2091
SAUR: SABB_02281(aphA)
SAUI: AZ30_14265
SER: SEA0010(aphA)
SHH: ShL2_02167(aphA)
SDT: SPSE_1794(aphA)
SWA: A284_12042
GYM: GYMC10_0161
PALB: EJC50_17330
PBK: Back11_32840 Back11_38870(ymdC)
PLYC: GXP70_15210
PTHI: NDS46_13665
PDY: QJQ58_08345 QJQ58_28650
PSPN: L1F29_28735
PPOG: QPK24_10940
PAUN: MJA45_10235
PHK: SK066_13680
STHO: QNH10_14140
LLA: L33782(ymdC)
LLK: LLKF_1253(ymdC)
LLT: CVCAS_1176(ymdC)
LLS: lilo_1100(ymdC)
LLD: P620_06885
LLX: NCDO2118_1221(ymdC)
LLM: llmg_1293
LLC: LACR_1330
LLR: llh_6710
LLN: LLNZ_06680
LLI: uc509_1219
LLW: kw2_1172
SPW: SPCG_1325 SPCG_1876
SPP: SPP_1930
SNU: SPNA45_00348
SSA: SSA_1830
SSUS: NJAUSS_0666
SUI: SSUJS14_0692
SGO: SGO_0628(ymdC)
SEZ: Sez_1628
SEQ: SZO_03450
SEZO: SeseC_02178
SEU: SEQ_1846
SMB: smi_1708(aphA)
STD: SPPN_09665
SIE: SCIM_0382
SIB: SIR_1285
SIU: SII_1309
SANG: SAIN_0411
SCG: SCI_0444
SCON: SCRE_0424
SCOS: SCR2_0424
STRN: SNAG_0434(neo)
SPEI: EHW89_01960
SPLR: C0J00_02060
SMEN: SAMEA4412692_1588(butA) SAMEA4412692_1589(ymdC)
SLAT: J4854_02790
SACO: SAME_00507(ymdC)
SACY: O6R09_03260(aph(3')-IIIa)
LJO: LJ_1688
LJF: FI9785_1471(aph)
LJH: LJP_1424c
LJN: T285_07210
LAC: LBA1348
LAD: LA14_1348
LAF: SD55_1334
LGA: LGAS_1458
LTA: H1A07_07220
EFAU: EFAU085_p3013(aphA)
EFU: HMPREF0351_12797(aphA) HMPREF0351_12830(aphA2)
ERAF: J9537_20510(aph(3')-IIIa)
SMAN: C12CBH8_05220(ymdC)
ALAC: JYE50_15120
AHC: JYE49_00220
PROM: QO263_02975
CBAF: JS518_09435
RHO: RHOM_01605
BLAR: LC508_10530
EBM: SG0102_14070(aphA)
ERM: EYR00_09540
AOC: Aocu_00810
EGD: GS424_013585
MTU: Rv3817
MTV: RVBD_3817
MTC: MT3925.1(aph)
MRA: MRA_3857(aph)
MTF: TBFG_13851
MTB: TBMG_03864
MTK: TBSG_03887
MTZ: TBXG_003834
MTG: MRGA327_23505
MTI: MRGA423_24085
MTE: CCDC5079_3547
MTUR: CFBS_4047(aph)
MTL: CCDC5180_3495
MTO: MTCTRI2_3896
MTD: UDA_3817
MTN: ERDMAN_4184
MTJ: J112_20525
MTUB: MT7199_3886
MTUC: J113_26725
MTUE: J114_20400
MTX: M943_19600
MTUH: I917_26845
MTUL: TBHG_03755
MTUT: HKBT1_4030(aph)
MTUU: HKBT2_4040(aph)
MTQ: HKBS1_4043(aph)
MBO: BQ2027_MB3847
MBB: BCG_3879
MBT: JTY_3881
MBM: BCGMEX_3880
MBK: K60_039600
MBX: BCGT_3681
MAF: MAF_38320
MMIC: RN08_4213
MCE: MCAN_38361
MCQ: BN44_120227
MCV: BN43_90334
MCX: BN42_90342
MCZ: BN45_110179
MORY: MO_003969
MYO: OEM_11520
MCHI: AN480_06785 AN480_29000
MSA: Mycsm_06204
MUL: MUL_5000
MMI: MMAR_5380
MMAE: MMARE11_52010
MLI: MULP_05659
MPSE: MPSD_55070
MSHO: MSHO_08310
MKN: MKAN_14280
MHAD: B586_02960
MDX: BTO20_33610
MSHG: MSG_04888
MFJ: MFLOJ_36310
MSTO: MSTO_40680
MKU: I2456_27545
MLW: MJO58_26940
MPAG: C0J29_29735
MGOR: H0P51_27540
MBAI: MB901379_04821(neo)
MLJ: MLAC_44020
MSHJ: MSHI_34300
MSPG: F6B93_22015
MOT: LTS72_25720
MCB: Mycch_5004
MAUU: NCTC10437_05419(neo)
MMOR: MMOR_07670
MFX: MFAL_38020
MIJ: MINS_38300
MARZ: MARA_44190
MAUB: MAUB_30310
MPHU: MPHO_39340
MMUC: C1S78_028100
MMAT: MMAGJ_28210
MSEI: MSEDJ_06470
MFLV: NCTC10271_00197(neo)
MCEE: MCEL_34050
MBRM: L2Z93_000829
MRF: MJO55_26025
MKR: MKOR_40480
MAB: MAB_0163c
MMV: MYCMA_0096
MABB: MASS_0161
MABL: MMASJCM_0165
MVM: MJO54_07505
CUR: cu1535(aph(3')-Ia)
CUA: CU7111_1481(aph(3')-Ia)
CSX: CSING_02010(aphA1)
CCJZ: ccrud_14555
CGLU: I6J20_10650 I6J20_10660
CWK: IA203_04670
CHAD: CHAD_02585(neo1)
CSUE: QP029_10675
CPYR: CYJ47_11065
NFA: NFA_38480(aph(3'))
NCY: NOCYR_3725
NBR: O3I_030060
NAH: F5544_30275
NAD: NCTC11293_05205(neo)
NIE: KV110_29075
NHU: H0264_30970
NGP: LTT66_24220
NSPU: IFM12276_17070
PDEF: P9209_15880
GOI: LK459_00010
TPR: Tpau_1464
DIZ: CT688_13520
SBH: SBI_05609
SRN: A4G23_05133(neo)
SALW: CP975_01435
SRIM: CP984_09245(aph(3')-VIII)
SFB: CP974_27395
SROI: IAG44_36080
SXN: IAG42_31220
SHK: J2N69_01555
KSK: KSE_41510
KIS: HUT16_19175
YIA: LO772_33025
ABRY: NYE86_35400
AGLA: OIE69_23520
MTS: MTES_1899
MIO: AOA12_18300
MIP: AXH82_04640
MCW: A8L33_09395
MPAL: BO218_08770
MAUR: BOH66_01830
MFOL: DXT68_15175
MOO: BWL13_02576(neo)
MWA: E4K62_17060
MPRT: ET475_03850
MOY: CVS54_03366(neo)
MSF: IT882_06765
MLZ: F6J85_16065
MCHN: HCR76_02755
MICS: C1N74_02210
MLIQ: NMQ05_16215
MTEC: OAU46_00630
MPAO: IZR02_15090
MAZA: NFX31_15200
MARK: QUC20_02015
MRG: SM116_03245
MINV: T9R20_02490
MEST: PTQ19_14040
ROE: Q0F99_16910
CUB: BJK06_05580
FRP: AX769_02465
FSB: GCM10025867_04500
AGY: ATC03_08620
ASOI: MTP13_12970
MDB: OVN18_10975
LVI: G7068_05495
LLUT: K1X41_02650
LARI: KI794_06920
HEH: L3i23_06970
AGX: AGREI_1209
HOM: OF852_04940
ARL: AFL94_01545 AFL94_17275
ARY: ATC04_16470
AAI: AARI_03100
GAR: AOZ07_01940
GLU: F0M17_01505
GPR: JQN66_01610
GMI: NMP99_01805
GMY: XH9_07065
GNC: QQS42_01610
KRH: KRH_10490
MTEU: R3I42_02775
XYL: ET495_07705
XYA: ET471_00320
CET: B8281_09980
CCEU: CBR64_01135
CPRT: FIC82_008950
SANW: G7063_05425
CFL: Cfla_1184
CFI: Celf_1130
CGA: Celgi_0943
CEZ: CBP52_08815
CELZ: E5225_12125
CEJ: GC089_12660
CELH: GXP71_10685
CIRA: LFM56_12285
CPAL: F1D97_15530
CDON: KKR89_05750
CWAN: KG103_06095
CWN: NP075_05975
CXIE: NP048_05975
OEK: FFI11_009870
PSEK: GCM125_23560
PBO: PACID_10930
AACI: ASQ49_15725
TDF: H9L22_03570
TLA: TLA_TLA_02719(neo)
TEJ: KDB89_08965
PRV: G7070_05975
NPC: KUV85_00315
KFL: Kfla_1280
KQI: F1D05_25900
THAO: NI17_007520
TALX: FOF52_05830
NDA: Ndas_3531
NAL: B005_4376
NAKE: KGD83_07540
NCG: KGD84_07025
SAIU: J4H86_03160
ACTQ: OG417_03685
SRO: Sros_0454 Sros_5038
NFS: OIE67_18435
FRA: Francci3_3368
NAKA: H7F38_03140
KRA: Krad_1799
SACC: EYD13_01980(neo)
AMI: Amir_1990
LED: BBK82_29490
AHM: TL08_05855
CROS: N8J89_09740
MTUA: CSH63_05730
MSAG: GCM10017556_22720
AFS: AFR_29645
PLAB: C6361_07070
PLAT: C6W10_03645
PRY: Prubr_49580
DVC: Dvina_05435
CAI: Caci_6179
GLY: K3N28_18390
STUC: G5S47_08045
BBB: BIF_01665
BNM: BALAC2494_01072
BLW: W7Y_0046
BLS: W91_0045
BNI: BANAN_00270
BANM: EN10_00245
BBP: BBPR_1808
BBI: BBIF_1747
BBF: BBB_1805(ymdC)
BPSP: AH67_00170
BSCA: BBSC_0104
BCHO: BcFMB_02555
BLEM: BL8807_09340
BEU: BE0216_02055
JTL: M6D93_06060
ATQ: GH723_07245(aph(3'))
CAU: Caur_2511
CHL: Chy400_2709
KBS: EPA93_24010
PMET: G4Y79_23865
DRA: DR_0066
DGE: Dgeo_2256
DDR: Deide_21650
DMR: Deima_0344
DPT: Deipr_1927
DGO: DGo_CA0345
DPD: Deipe_2720
DSW: QR90_03680
DCH: SY84_07575
DAB: AUC44_14545
DPU: SU48_00375
DEZ: DKM44_13465
DWU: DVJ83_11460
DFC: DFI_01070
DEIN: DAAJ005_14275
DGA: DEGR_29090
DPH: EHF33_12990
DRD: LMT64_09225
DRB: N0D28_12950
DAH: DAETH_00730
DAQU: M8445_06045
DTAE: LAJ19_11510
DMB: E5F05_20635
MSV: Mesil_0966
RUFI: K0V07_16440
CVL: J8C06_13920
MICA: P0L94_00625
PGOL: K6V26_03545
BUY: D8S85_06000(aph(3')-IIIa)
COY: HF329_05125
SRHO: HH216_17180
BPOR: BPO_2057(aph3-I)
 » show all
Reference
1  [PMID:4970990]
  Authors
Doi O, Ogura M, Tanaka N, Umezawa H.
  Title
Inactivation of kanamycin, neomycin, and streptomycin by enzymes obtained in cells of Pseudomonas aeruginoa.
  Journal
Appl Microbiol 16:1276-81 (1968)
Reference
2  [PMID:13416259]
  Authors
DOLIN MI.
  Title
The Streptococcus faecalis oxidases for reduced diphosphopyridine nucleotide.  III.  Isolation and properties of a flavin peroxidase for reduced diphosphopyridine nucleotide.
  Journal
J Biol Chem 225:557-73 (1957)
Other DBs
ExplorEnz - The Enzyme Database: 2.7.1.95
IUBMB Enzyme Nomenclature: 2.7.1.95
ExPASy - ENZYME nomenclature database: 2.7.1.95
BRENDA, the Enzyme Database: 2.7.1.95
CAS: 62213-36-9

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Chemical substance (4)   
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Gene (1103)   
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   KEGG GENES (659)   
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DNA sequence (1967)   
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   GenBank (948)   
   EMBL (862)   
3D Structure (17)   
   PDB (17)   
Protein domain (4)   
   InterPro (4)   
All databases (5421)   

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