KEGG ENZYME: 2.7.7.97

ENZYME: 2.7.7.97

Entry

EC 2.7.7.97                 Enzyme

Name

3-hydroxy-4-methylanthranilate adenylyltransferase;
acmA (gene name);
sibE (gene name);
actinomycin synthase I;
4-MHA-activating enzyme;
ACMS I;
actinomycin synthetase I;
4-MHA pentapeptide lactone synthase AcmA

Class

Transferases;
Transferring phosphorus-containing groups;
Nucleotidyltransferases

Sysname

ATP:3-hydroxy-4-methylanthranilate adenylyltransferase

Reaction(IUBMB)

ATP + 3-hydroxy-4-methylanthranilate = diphosphate + 3-hydroxy-4-methylanthranilyl-adenylate [RN:R11554]

Reaction(KEGG)

R11554

Substrate

ATP [CPD:C00002];
3-hydroxy-4-methylanthranilate [CPD:C03986]

Product

diphosphate [CPD:C00013];
3-hydroxy-4-methylanthranilyl-adenylate

Comment

The enzyme, characterized from the bacteria Streptomyces anulatus and Streptosporangium sibiricum, activates 3-hydroxy-4-methylanthranilate, a precursor of actinomycin antibiotics and the antitumor antibiotic sibiromycin, to an adenylate form, so it can be loaded onto a dedicated aryl-carrier protein.

History

EC 2.7.7.97 created 2016

Orthology

K21284

3-hydroxy-4-methylanthranilate adenylyltransferase

Genes

KGR:	JJJ10_13575

KLM:	BWI76_15080

VRU:	RND59_08015

PCEL:

HUB94_18035

NBR:	O3I_021160

NOZ:	DMB37_35945

NHU:	H0264_30690

SANL:

KZO11_29675

SGRF:

SGFS_035550 SGFS_087230

SCB:	SCAB_69851

SRC:	M271_02960

SBH:

SBI_01606

SLD:	T261_0691 T261_2098

SPAV:

Spa2297_02690 Spa2297_26635

SPHW:

NFX46_21455

SLS:	SLINC_4426 SLINC_6767

SPLU:

LK06_031595

SGV:	B1H19_08420

SFK:	KY5_6714 KY5_6734

SDX:	C4B68_39065

SKA:	CP970_06985 CP970_07075

SBY:	H7H31_03490

SFEU:

IM697_30790

SAUH:

SU9_026730

SLF:	JEQ17_24455

SDEC:

L3078_11315

SRUG:

F0345_28460

SAKB:

K1J60_34225

SCAE:

IHE65_34330

SANT:

QR300_02495

SYUN:

MOV08_33800

SJN:	RI060_03955

SHAU:

K9S39_40180

SKG:	KJK29_03005

SCOA:

QU709_20155 QU709_24610

SLAI:

P8A22_34825

STRZ:

OYE22_03360

KSK:	KSE_65160 KSE_67410

KIS:	HUT16_30905 HUT16_31040 HUT16_31825

STRI:

C7M71_007290

STRH:

GXP74_15530 GXP74_26115

ABRY:

NYE86_02840

RTX:	TI83_07420

RTC:	APU90_09660

MPOR:

KW076_03645

CXIE:

NP048_18850

KFL:

Kfla_6074

KSL:	OG809_05965

NAKE:

KGD83_15170

AROO:

NQK81_43525

SESP:

BN6_21380 BN6_63880

SSYI:

EKG83_30965 EKG83_36315

KAL:

KALB_2251

KUT:	JJ691_50990

KPHY:

AOZ06_26445

AHM:	TL08_06700

ACTI:

UA75_06460

ACAD:

UA74_06460

AHG:	AHOG_06230(lcfB1)

ALO:

CRK56726

ACTU:

Actkin_05211(bclA_2)

UME:	RM788_01320 RM788_07320

STP:	Strop_2497

SAQ:

Sare_2681

MAU:	Micau_3489

MIL:

ML5_4902

MICB:

MicB006_2544

MTUA:

CSH63_07600

MICH:

FJK98_16405

MTEM:

GCE86_13445

MCAB:

HXZ27_18170

MSAG:

GCM10017556_53700

MCHL:

PVK74_07690

MFEU:

H1D33_23030

MHAW:

RMN56_24410

ACTN:

L083_2872

PFLA:

Pflav_007540

PSUU:

Psuf_064580

CATI:

CS0771_77100

DVC:	Dvina_13840 Dvina_29170

DFU:	Dfulv_12485

DAUR:

Daura_12975

DMAT:

Dmats_13375 Dmats_24410

CAI:

Caci_3250

» show all

Reference

1 [PMID:10212227]

Authors

Pfennig F, Schauwecker F, Keller U

Title

Molecular characterization of the genes of actinomycin synthetase I and of a 4-methyl-3-hydroxyanthranilic acid carrier protein involved in the assembly of the acylpeptide chain of actinomycin in Streptomyces.

Journal

J Biol Chem 274:12508-16 (1999)
DOI:10.1074/jbc.274.18.12508

Sequence

[ag:ADG27357]

Reference

2 [PMID:21612226]

Authors

Giessen TW, Kraas FI, Marahiel MA

Title

A four-enzyme pathway for 3,5-dihydroxy-4-methylanthranilic acid formation and incorporation into the antitumor antibiotic sibiromycin.

Journal

Biochemistry 50:5680-92 (2011)
DOI:10.1021/bi2006114