KEGG   ENZYME: 2.8.1.15
Entry
EC 2.8.1.15                 Enzyme                                 
Name
tRNA-5-methyluridine54 2-sulfurtransferase;
TtuA
Class
Transferases;
Transferring sulfur-containing groups;
Sulfurtransferases
Sysname
[TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH:tRNA (5-methyluridine54-2-O)-sulfurtransferase
Reaction(IUBMB)
ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluracil54 in tRNA + H2O = AMP + diphosphate + 5-methyl-2-thiouracil54 in tRNA + [TtuB sulfur-carrier protein]-Gly-Gly
Substrate
ATP [CPD:C00002];
[TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH;
5-methyluracil54 in tRNA;
H2O [CPD:C00001]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
5-methyl-2-thiouracil54 in tRNA;
[TtuB sulfur-carrier protein]-Gly-Gly
Comment
The enzyme, found in thermophilic bacteria and archaea, modifies the ribothymidine (5-methyluridine) residue at position 54 of tRNAs. Contains zinc and an [4Fe-4S] cluster. Some organisms, such as the archaeon Pyrococcus horikoshii, do not have a TtuB sulfur-carrier protein, and appear to use sulfide as the sulfur source.
History
EC 2.8.1.15 created 2017
Orthology
K21947  tRNA-5-methyluridine54 2-sulfurtransferase
Genes
HMR: Hipma_0320
DDS: Ddes_2277
DFI: AXF13_12765
DPG: DESPIGER_2017
DEF: CNY67_14630
DFL: DFE_1672
PSYF: N1030_12685
DSB: LN040_12120
DMA: DMR_30620
DCB: C3Y92_06855
DGG: DGI_0441
DDE: Dde_3611
DMS: E8L03_16510
DSA: Desal_1104
DHY: DESAM_21692
DAF: Desaf_3387
DAS: Daes_2444
DPI: BN4_10998
DEJ: AWY79_08310
PPRF: DPRO_2984
PSEL: GM415_08370
DDN: DND132_1127
PSEF: PSDVSF_11620
PPOR: JCM14722_08630
PNW: SYK_12880
PTHE: LF599_12735
DSX: GD604_04385
DSD: GD606_16310
PBIZ: LWC08_08885
DVU: DVU_0135
DVL: Dvul_2830
DVM: DvMF_2330
DVG: Deval_0160
CLIH: KPS_002766
TMUR: JBF11_06245
DBA: Dbac_1482
DOA: AXF15_08930
DESU: NLA06_06355
THEW: TDMWS_12930
DRT: Dret_1363
SFU: Sfum_0716
DAX: FDQ92_08100
DBR: Deba_3267
DMP: FAK_00260
TID: Thein_0509
TOP: TOPB45_1141
TCM: HL41_03015
THET: F1847_05360
CTHI: THC_0836
TAV: G4V39_09445
TMAI: FVE67_05585
HYI: K2M58_11170
DKU: Desku_0385
DAU: Daud_1159
TMR: Tmar_2076
THEF: E1B22_01925
THEP: DYI95_010320
TCP: Q5761_10655
CLE: Clole_0286
TTE: TTE0073(MesJ) TTE2470(MesJ4)
TEX: Teth514_1159 Teth514_1780
THX: Thet_0071 Thet_1752
TPD: Teth39_2172
TIT: Thit_0462
TMT: Tmath_0553
TBO: Thebr_2218
TWI: Thewi_0085 Thewi_2475
TKI: TKV_c00760
ADG: Adeg_0170
TPZ: Tph_c13040(ttcA1)
TOC: Toce_1816
NTH: Nther_0414
NCD: ACONDI_01569(ttcA)
CSC: Csac_0104
ATE: Athe_0195
COB: COB47_0185
CHD: Calhy_2432
COW: Calow_0133
CKI: Calkr_0169
CLC: Calla_2160
CKN: Calkro_2377
CCHA: ELD05_00515
CDIA: CaldiYA01_22130
CNAG: OTJ99_000205
CMOR: OTK00_000065
CDAN: SOJ16_000199
ERZ: ER308_20645
EUZ: DVS28_a2562
MHAS: MHAS_03206(ttcA)
MPRN: Q3V37_25750
ABAI: IMCC26256_111163
AYM: YM304_26130
ATQ: GH723_09465
IAM: HC251_10980
IMA: PO878_10745
DHI: LH044_02435
PSUB: Pelsub_P1449
THUG: KNN16_05655
TTH: TT_C0106
TTJ: TTHA0477(TTHA0477)
TTS: Ththe16_0475
TTL: TtJL18_1605
TSC: TSC_c19640
THC: TCCBUS3UF1_17660
TOS: Theos_1828
TAQ: TO73_1857
TBC: A0O31_01884
TANT: KNN15_01330
OPR: Ocepr_0668
MHD: Marky_0475
TNR: Thena_1222
TACI: TDSAC_1197
TAQU: KDW03_10845
TLI: Tlie_1905
AMO: Anamo_0924
AAE: aq_1333
TAL: Thal_0032
TRD: THERU_05825
SUL: SYO3AOP1_1506
SAF: SULAZ_1054
PMX: PERMA_1248
TAM: Theam_0639
DTE: Dester_0641
TTK: TST_0665 TST_1605
TMA: TM0197
TMM: Tmari_0195
TMI: THEMA_03745
TMW: THMA_0204
TMQ: THMB_0204
TMX: THMC_0204
TPT: Tpet_0727
TNP: Tnap_0827
TRQ: TRQ2_0751
TNA: CTN_0489
THQ: T2812B_03815
THZ: CELL2_03775
THR: TRQ7_03910
TLE: Tlet_0417
TTA: Theth_0810
PHY: AJ81_06375
TME: Tmel_1006
TAF: THA_1285
THP: BG95_05790
THER: Y592_05865
FNO: Fnod_1006
FPE: Ferpe_1290
FIA: NA23_06435
FRR: IB67_07955
KOL: Kole_1722
KPF: IX53_04615
CEX: CSE_02580
DDF: DEFDS_0604
DAP: Dacet_1666
GTL: EP073_10645
DTH: DICTH_1565
DTU: Dtur_1672
TYE: THEYE_A1033
NDE: NIDE3396
NMV: NITMOv2_3760
NIO: NITINOP_2657
NJA: NSJP_0222
NKF: Nkreftii_001909
NIF: W02_15860
NTI: DNFV4_03592
NALL: PP769_08370
NNEO: PQG83_19610
LFC: LFE_0457
LFI: LFML04_0439
LFP: Y981_02255
LEG: ABH19_02680
ALAM: RT761_01994(tilS_1)
FSH: Q2T83_13940
MJA: MJ_1478
MFE: Mefer_1461
MVU: Metvu_1262
MFS: MFS40622_0977
MIF: Metin_0254
MJH: JH146_1505
MESG: MLAUSG7_1398
MESA: MLASG1_0825
MIG: Metig_1781
METF: CFE53_02565
MFV: Mfer_1176
MKA: MK0144
AFU: AF_1321
AFG: AFULGI_00014320
APO: Arcpr_0758
AVE: Arcve_1701
FPL: Ferp_1084
GAC: GACE_0225
GAH: GAH_00072
PFU: PF0273
PFI: PFC_00430
PHO: PH0300(PH0300)
PAB: PAB1092
PYN: PNA2_0919
PYA: PYCH_13740
PYS: Py04_0428
PYC: TQ32_00910
TKO: TK1556
TON: TON_1717
TGA: TGAM_2130
TSI: TSIB_0454
TBA: TERMP_01945
THE: GQS_06720
THA: TAM4_293
THM: CL1_0970
TLT: OCC_10073
THS: TES1_1901
TNU: BD01_1355
TEU: TEU_02835
TGY: X802_04030
THV: ADU37_CDS03840
TCH: CHITON_0494
TPEP: A0127_07390
TPIE: A7C91_03480
TGG: A3K92_00070
TCE: A3L02_07000
TBS: A3L01_09550
THH: CDI07_09470
TSL: A3L11_02990
TTD: A3L14_00535
TPRF: A3L09_01890
TRL: A3L10_08970
TPAF: A3L08_02415
THY: A3L12_00230
TIC: FH039_01110
TCQ: TIRI35C_0033
THEM: FPV09_09325
THEI: K1720_05390
PPAC: PAP_01580
ABI: Aboo_0768
ACF: AciM339_0299
APE: APE_2086
ACJ: ACAM_1305
SMR: Smar_0001 Smar_0396
SHC: Shell_0406 Shell_0796
IHO: Igni_0707
IIS: EYM_07570
DKA: DKAM_0492 DKAM_0722
DFD: Desfe_0441 Desfe_0784
DMU: Desmu_0403
IAG: Igag_0555
THG: TCELL_0953 TCELL_1396
HBU: Hbut_0094 Hbut_0591
PFM: Pyrfu_1651
PDL: Pyrde_0636
PABI: PABY_03950
PARE: PYJP_16010
STO: STK_06300
SOH: D1869_03025
SAI: Saci_0378
SACN: SacN8_01855
SACR: SacRon12I_01855
SACS: SUSAZ_01940
SULL: EWF20_04545
MSE: Msed_1487
MEMJ: MJ1HA_1702
MCN: Mcup_0774
MHK: DFR87_11280
MPRU: DFR88_10870
MTEN: GWK48_01065
MJN: MjAS7_2582
AHO: Ahos_0949
AMAN: B6F84_08595
ABRI: DFR85_05325
ASUL: DFR86_07605
AAMB: D1866_12655
ACIH: HS5_23270
SAZO: D1868_00540
CSTY: KN1_16130
STEP: IC006_1661
SMET: RQ359_000279
PIS: Pisl_0017
PCL: Pcal_2018
POG: Pogu_1236
VDI: Vdis_2265
TPE: Tpen_0923 Tpen_1268
THB: N186_06180
TCB: TCARB_0298
THEL: IG193_01375 IG193_03140
ASC: ASAC_0917
CCAI: NAS2_0586
KCR: Kcr_0210
NEQ: NEQ283 NEQ523
LOB: NEF87_001826 NEF87_002962
PSYT: DSAG12_00132 DSAG12_00901
 » show all
Reference
1  [PMID:16547008]
  Authors
Shigi N, Sakaguchi Y, Suzuki T, Watanabe K
  Title
Identification of two tRNA thiolation genes required for cell growth at extremely high temperatures.
  Journal
J Biol Chem 281:14296-306 (2006)
DOI:10.1074/jbc.M511675200
  Sequence
[tth:TT_C0106]
Reference
2  [PMID:16317006]
  Authors
Shigi N, Suzuki T, Terada T, Shirouzu M, Yokoyama S, Watanabe K
  Title
Temperature-dependent biosynthesis of 2-thioribothymidine of Thermus thermophilus tRNA.
  Journal
J Biol Chem 281:2104-13 (2006)
DOI:10.1074/jbc.M510771200
Reference
3  [PMID:23444054]
  Authors
Nakagawa H, Kuratani M, Goto-Ito S, Ito T, Katsura K, Terada T, Shirouzu M, Sekine S, Shigi N, Yokoyama S
  Title
Crystallographic and mutational studies on the tRNA thiouridine synthetase TtuA.
  Journal
Proteins 81:1232-44 (2013)
DOI:10.1002/prot.24273
  Sequence
[pho:PH0300]
Reference
4  [PMID:27710943]
  Authors
Chen M, Narai S, Omura N, Shigi N, Chimnaronk S, Tanaka Y, Yao M
  Title
Crystallographic study of the 2-thioribothymidine-synthetic complex TtuA-TtuB from Thermus thermophilus.
  Journal
Acta Crystallogr F Struct Biol Commun 72:777-781 (2016)
DOI:10.1107/S2053230X16014242
  Sequence
[tth:TT_C0106]
Other DBs
ExplorEnz - The Enzyme Database: 2.8.1.15
IUBMB Enzyme Nomenclature: 2.8.1.15
ExPASy - ENZYME nomenclature database: 2.8.1.15
BRENDA, the Enzyme Database: 2.8.1.15

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Gene (684)   
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   KEGG GENES (303)   
   KEGG MGENES (264)   
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Protein sequence (26)   
   UniProt (19)   
   SWISS-PROT (1)   
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DNA sequence (16)   
   GenBank (8)   
   EMBL (8)   
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