Entry
Name
tRNA-2-methylthio-N6-dimethylallyladenosine synthase;
MiaB;
2-methylthio-N-6-isopentenyl adenosine synthase;
tRNA-i6A37 methylthiotransferase;
tRNA (N6-dimethylallyladenosine37):sulfur-(sulfur carrier),S-adenosyl-L-methionine C2-methylthiotransferase
Class
Transferases;
Transferring sulfur-containing groups;
Transferring alkylthio groups
BRITE hierarchy
Sysname
tRNA N6-(3-methylbut-2-en-1-yl)-adenine37:sulfur-(sulfur carrier),S-adenosyl-L-methionine C2-(methylsulfanyl)transferase
Reaction(IUBMB)
N6-(3-methylbut-2-en-1-yl)-adenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = N6-(3-methylbut-2-en-1-yl)-2-(methylsulfanyl)adenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenine + electron acceptor (overall reaction) [RN:
R10645 ];
(1a) N6-(3-methylbut-2-en-1-yl)-adenine37 in tRNA + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced electron acceptor = N6-(3-methylbut-2-en-1-yl)-2-thioadenine37 in tRNA + (sulfur carrier) + L-methionine + 5'-deoxyadenine + electron acceptor [RN:
R10646 ];
(1b) S-adenosyl-L-methionine + N6-(3-methylbut-2-en-1-yl)-2-thioadenine37 in tRNA = S-adenosyl-L-homocysteine + N6-(3-methylbut-2-en-1-yl)-2-(methylsulfanyl)adenine37 in tRNA [RN:
R10647 ]
Reaction(KEGG)
Substrate
N6-(3-methylbut-2-en-1-yl)-adenine37 in tRNA;
sulfur-(sulfur carrier);
S-adenosyl-L-methionine [CPD:
C00019 ];
reduced electron acceptor;
N6-(3-methylbut-2-en-1-yl)-2-thioadenine37 in tRNA
Product
N6-(3-methylbut-2-en-1-yl)-2-(methylsulfanyl)adenine37 in tRNA;
S-adenosyl-L-homocysteine [CPD:
C00021 ];
(sulfur carrier);
L-methionine [CPD:
C00073 ];
5'-deoxyadenine;
electron acceptor;
N6-(3-methylbut-2-en-1-yl)-2-thioadenine37 in tRNA
Comment
This bacterial enzyme binds two [4Fe-4S] clusters as well as the transferred sulfur [3]. The enzyme is a member of the superfamily of S-adenosyl-L-methionine-dependent radical (radical AdoMet) enzymes. The sulfur donor is believed to be one of the [4Fe-4S] clusters, which is sacrificed in the process, so that in vitro the reaction is a single turnover. The identity of the electron donor is not known.
History
EC 2.8.4.3 created 2014, modified 2015
Orthology
K06168 tRNA-2-methylthio-N6-dimethylallyladenosine synthase
Genes
ECOO : ECRM13514_0686(yleA)
ECOH : ECRM13516_0632(yleA)
ECW : EcE24377A_0690(miaB)
EDJ : ECDH1ME8569_0630(miaB)
ECOI : ECOPMV1_00671(miaB)
REE : electrica_03645(miaB)
RTG : NCTC13098_05342(miaB)
CLAP : NCTC11466_03291(miaB)
KIE : NCTC12125_02320(miaB)
AHN : NCTC12129_03539(miaB)
METY : MRY16398_40720(miaB)
PSHI : SAMEA2665130_2231(miaB)
YPG : YpAngola_A1829(miaB)
YPI : YpsIP31758_2915(miaB)
SMW : SMWW4_v1c11880(miaB)
SFJ : SAMEA4384070_1269(miaB)
SOF : NCTC11214_05713(miaB)
PAQU : DMB82_0014505(miaB)
DIC : Dpoa569_002578(miaB)
BAPF : BUMPF009_CDS00158(ylea)
BAPG : BUMPG002_CDS00158(ylea)
BAPU : BUMPUSDA_CDS00158(ylea)
BAPW : BUMPW106_CDS00158(ylea)
TPTY : NCTC11468_01170(miaB)
PHEI : NCTC12003_02969(miaB)
PRJ : NCTC6933_01068(miaB)
LRI : NCTC12151_02231(miaB)
HIC : NTHIC486_01024(miaB)
HPIT : NCTC13334_01132(miaB)
HHZ : NCTC10839_01059(miaB)
HAEG : NCTC8502_01955(miaB)
PDAG : 4362423_01250(miaB)
ADP : NCTC12871_00169(miaB)
ALIG : NCTC10568_01641(miaB)
ASEG : NCTC10977_01894(miaB)
AVT : NCTC3438_01080(miaB)
RPNE : NCTC8284_03181(miaB_2)
PAET : NCTC13378_00320(miaB)
XFU : XFF4834R_chr24450(miaB)
XPH : XppCFBP6546_00810(miaB)
XHD : LMG31886_18360(miaB)
SACZ : AOT14_13960(rimO_1)
LLZ : LYB30171_00877(miaB)
VAU : VANGNB10_cI0837c(miaB)
VPL : SA104470976_01806(miaB)
PVD : CFBP1590__4689(miaB)
PPRC : PFLCHA0_c54020(miaB)
PMUD : NCTC8068_04624(miaB)
PFW : PF1751_v1c48200(miaB)
PPUU : PputUW4_04763(miaB)
PTRT : HU722_0025935(miaB)
PTK : EXN22_10140 EXN22_24180(miaB)
PTW : TUM18999_09850(miaB)
PTAE : NCTC10697_00639(miaB)
MSHE : MAALD49_27150(miaB)
PSYC : DABAL43B_2344(miaB)
MCAT : MC25239_01397(miaB)
MCUN : NCTC10297_00723(miaB)
SCAA : TUM17387_09970(miaB)
SALH : HMF8227_00994(miaB)
CBS : COXBURSA331_A0683(miaB)
LLG : 44548918_01025(miaB)
LJR : NCTC11533_01211(miaB)
LCJ : NCTC11976_01995(miaB)
LWA : SAMEA4504053_1511(miaB)
LSS : NCTC12082_00741(miaB)
LADL : NCTC12735_01197(miaB)
LANT : TUM19329_16310(miaB)
MISZ : MishRS11D_35790(miaB)
TIB : THMIRHAM_18130(miaB)
TSE : THMIRHAS_20960(miaB)
TZO : THMIRHAT_18440(miaB)
TFRI : Thiofri_04518(miaB)
TNI : TVNIR_2544(miaB_[H])
GAI : IMCC3135_10265(miaB)
THIC : TspCOW1_16780(miaB)
HAXI : HAALTHF_46510n(miaB)
ALCA : ASALC70_03388(miaB_1)
AEL : NCTC12917_01076(miaB)
CHJ : NCTC10426_00282(miaB)
CDIZ : CEDIAZO_02439(miaB_2)
NMM : NMBM01240149_0323(miaB)
NMS : NMBM01240355_1796(miaB)
NMQ : NMBM04240196_1804(miaB)
NMZ : NMBNZ0533_0461(miaB)
NMX : NMA510612_0650(miaB)
NWE : SAMEA3174300_1955(miaB)
NZO : SAMEA4504057_0016(miaB)
NCI : NCTC10296_01087(miaB)
NCZ : NCTC10294_01326(miaB)
NANI : NCTC12227_00102(miaB)
ECOR : SAMEA4412678_0045(miaB)
IOD : EJO50_03820 EJO50_15310(miaB)
BMV : BMASAVP1_A2720(miaB)
BML : BMA10229_A2358(miaB)
BPM : BURPS1710b_0889(miaB)
BPL : BURPS1106A_0724(miaB)
BUE : BRPE67_ACDS03990(miaB)
PLG : NCTC10937_04767(miaB)
LMIR : NCTC12852_02421(miaB)
BUO : BRPE64_ACDS04240(miaB)
BTRM : SAMEA390648702138(miaB)
AXX : ERS451415_04998(miaB)
LIM : L103DPR2_02719(miaB)
LIH : L63ED372_02471(miaB)
SDR : SCD_n00241 SCD_n02591(miaB)
URU : DSM104443_00385(miaB)
UPL : DSM104440_00450(miaB)
FPHO : SHINM1_000820(miaB)
APLT : ANPL_03070(miaB_1) ANPL_03110(miaB_2)
RBS : RHODOSMS8_03203(miaB)
SMEL : SM2011_c01115(miaB)
SFD : USDA257_c00410(miaB)
SAME : SAMCFNEI73_Ch0415(miaB)
AGR : AGROH133_03545(miaB)
ALF : CFBP5473_14175(miaB)
AVV : RvVAT039_20090(miaB)
AVF : RvVAR031_07240(miaB)
ASAL : CFBP5507_00160(miaB)
RPUS : CFBP5875_00160(miaB)
ALEG : CFBP4996_00160(miaB)
REL : REMIM1_CH00382(miaB)
REP : IE4803_CH00404(miaB)
REI : IE4771_CH00413(miaB)
RTR : RTCIAT899_CH02415(miaB)
RGA : RGR602_CH00405(miaB)
RPHA : AMC79_CH00417(miaB)
RLW : RlegWSM1455_00100(miaB)
RBW : RLCC275e_00105(miaB)
NEN : NCHU2750_00420(miaB)
LAS : CLIBASIA_01565(miaB)
BRAD : BF49_0031 BF49_7356
BSEP : HAP48_0016335(miaB)
BCAN : BcanWSM471_00390(miaB)
VGO : GJW-30_1_00550(miaB)
BBK : BARBAKC583_1243(miaB)
BEZ : NCTC12898_00117(miaB_1)
MAQU : Maq22A_1p32120(miaB)
MMES : MMSR116_27715(miaB)
MTUN : MTUNDRAET4_2485(miaB)
FIL : BN1229_v1_2221(miaB)
FIY : BN1229_v1_2220(miaB)
BVY : NCTC9239_03313(miaB)
TSV : DSM104635_03822(miaB)
PHP : PhaeoP97_02884(miaB)
PPIC : PhaeoP14_00389(miaB)
SINL : DSM14862_02335(miaB)
SPSE : SULPSESMR1_02301(miaB)
RMM : ROSMUCSMR3_00933(miaB)
AHT : ANTHELSMS3_01875(miaB)
LALG : LentiSH36_00748(miaB)
LVS : LOKVESSMR4R_02226(miaB)
AALK : LGT41_0006625(miaB)
SBIN : SBA_ch1_26170(miaB)
SFLA : SPHFLASMR4Y_00689(miaB)
EBLA : JGUZn3_18550(miaB_2)
HPO : HMPREF4655_20511(miaB)
HPX : HMPREF0462_0322(miaB)
HCL : NCTC13205_01105(miaB_2)
HSH : NHP194022_14420(miaB)
HPUL : NCTC13154_00174(miaB_1)
SULG : FJR48_09590(miaB) FJR48_11900(miaB) FJR48_12120(miaB)
CJV : MTVDSCj20_0431(miaB)
CFV : CFVI03293_1415(miaB)
CAMP : CFT03427_1340(miaB)
CCOC : CCON33237_0867(miaB)
CLR : UPTC16701_0327(miaB)
CLM : UPTC16712_0330(miaB)
CAMR : CAQ16704_0332(miaB)
CHV : CHELV3228_0827(miaB)
CPIN : CPIN18020_1181(miaB)
CAMZ : CVIC12175_0378(miaB)
GBN : GEOBRER4_07660(miaB)
PPOR : JCM14722_03640(miaB)
SBF : JCM31447_06140(miaB)
BAZ : BAMTA208_08635(ymcB)
BSON : S101395_03027(miaB)
BSTR : QI003_09100(miaB) QI003_12525(miaB)
BFD : NCTC4823_02404(miaB)
CSPG : LS684_08685(miaB) LS684_20750(miaB)
PARG : PspKH34_25310(miaB)
BLEN : NCTC4824_02590(miaB_1)
SAX : USA300HOU_1224(miaB)
SUQ : HMPREF0772_11936(miaB)
SAUT : SAI1T1_2009290(miaB)
SAUJ : SAI2T2_1009310(miaB)
SAUK : SAI3T3_1009300(miaB)
SAUQ : SAI4T8_1009290(miaB)
SAUV : SAI7S6_1009300(miaB)
SAUW : SAI5S5_1009260(miaB)
SAUX : SAI6T6_1009270(miaB)
SAUY : SAI8T7_1009300(miaB)
SDP : NCTC12225_01706(miaB_2)
SPIC : SAMEA4384060_1635(miaB_2)
SSH : NCTC13712_01237(miaB_1)
SSIM : SAMEA4384339_1177(miaB_1)
SSTE : SAMEA4384403_1504(miaB_2)
BAYD : BSPP4475_09405(miaB)
PPQ : PPSQR21_020510(miaB)
PNK : AASFL403_09780(miaB)
COHN : KCTCHS21_37480(miaB)
CCOH : SAMEA4530647_1126(miaB_1)
CRW : CROST_024330(miaB_2)
CAUN : CLAUR_006650(miaB_1)
CGEL : psyc5s11_26780(miaB)
HHW : NCTC503_01378(miaB_1)
CLO : HMPREF0868_0882(miaB)
SMAN : C12CBH8_09440(miaB)
PFAA : MM59RIKEN_14920(miaB)
VFA : MM35RIKEN_16990(miaB)
VCOP : MM50RIKEN_18960(miaB)
BPRO : PMF13cell1_03828(miaB)
BLUI : Blut17040_15330(miaB)
CSCI : HDCHBGLK_03011(miaB)
CHYL : CE91St63_17320(miaB)
ACEL : acsn021_28940(miaB)
ACHT : bsdcttw_32610(miaB)
CBAR : PATL70BA_0273(miaB)
AMIC : Ami3637_02815(miaB)
ABUT : Ami103574_06205(miaB)
ETM : CE91St48_21180(miaB)
GFE : Gferi_27225 Gferi_27275
PDF : CD630DERM_19860(miaB)
PHX : KGNDJEFE_01545(miaB)
FAA : HMPREF0389_00572(miaB)
MTHO : MOTHE_c10720(miaB2)
MTHZ : MOTHA_c11610(miaB2)
CDIA : CaldiYA01_10170(miaB)
PHAR : NCTC13077_00878(miaB_1)
PIV : NCTC13079_00827(miaB_2)
VRM : 44547418_00885(miaB_1)
VDN : NCTC11831_01076(miaB_2)
DHO : Dia5BBH33_11670(miaB)
MANA : MAMMFC1_03808(miaB_3)
STED : SPTER_19240(miaB_1)
PFAC : PFJ30894_01727(miaB)
AARG : Aargi30884_07290(miaB)
ABSI : A9CBEGH2_09130(miaB)
FIT : Fi14EGH31_06010(miaB)
PCAT : Pcatena_07770(miaB_3)
RTS : CE91St31_17190(miaB)
MMI : MMAR_1566(miaB) MMAR_1979
MMAE : MMARE11_14790(miaB) MMARE11_19030
MLI : MULP_01698(miaB_1) MULP_02145(miaB_2)
MPSE : MPSD_19930(miaB_1) MPSD_42810(miaB_2)
MSHO : MSHO_52090(miaB) MSHO_56540
MGOR : H0P51_02340(miaB) H0P51_10950(miaB)
MBAI : MB901379_01391(miaB_1) MB901379_01799(miaB_2)
MHEK : JMUB5695_03158(miaB)
MKY : IWGMT90018_38480(miaB)
MPHL : MPHLCCUG_03139(miaB_2)
MTHN : 4412656_02746(miaB)
MAUU : NCTC10437_02252(miaB)
MFLV : NCTC10271_03130(miaB)
MSAL : DSM43276_02830(miaB)
MTER : 4434518_02425(miaB)
CDIP : ERS451417_01471(miaB)
COI : CPCIP5297_06485(miaB)
CUN : Cul210932_1427(miaB)
CUQ : Cul210931_1316(miaB)
CUJ : CUL131002_1346c(miaB)
CMIN : NCTC10288_00985(miaB)
CRL : NCTC7448_02094(miaB)
CCYS : SAMEA4530656_0902(miaB)
NFR : ERS450000_00063(miaB)
NAD : NCTC11293_05190(miaB)
NWL : NWFMUON74_23360(miaB)
NSPU : IFM12276_17210(miaB)
RCR : NCTC10994_00846(miaB)
RANT : RHODO2019_05280(miaB)
TPUL : TPB0596_27920(miaB)
SAMB : SAM23877_5490(miaB)
SLE : sle_18840(sle_18840)
SGM : GCM10017557_21240(miaB)
STUI : GCM10017668_52650(miaB)
SGOB : test1122_21310(miaB)
SXT : KPP03845_105485(miaB)
MSUW : GCM10025863_11640(miaB)
FSB : GCM10025867_24080(miaB)
AMAU : DSM26151_11910(miaB)
AMAV : GCM10025877_30230(miaB)
MALK : MalAC0309_1115(miaB)
PSEV : USB125703_02125(miaB)
LYH : FrondiHNR_05020(miaB)
AAGI : NCTC2676_1_00982(miaB)
PSNI : NIBR502771_18355(miaB)
GCR : GcLGCM259_0887(miaB)
MICK : B1A86_00003530(miaB)
RDN : HMPREF0733_11583(miaB)
DCO : SAMEA4475696_1477(miaB)
DSS : GCM25873_11760(miaB)
PAK : HMPREF0675_4076(miaB)
CGRN : 4412665_01210(miaB)
PAUS : NCTC13651_01664(miaB)
PPC : HMPREF9154_1375(miaB)
RAIN : Rai3103_15540(miaB)
BROO : brsh051_26250(miaB)
NAQU : ENKNEFLB_03963(miaB)
AGRA : AGRA3207_006468(miaB)
ACTU : Actkin_01633(miaB_1)
MSAG : GCM10017556_41030(miaB)
MCU : HMPREF0573_11557(miaB)
TBW : NCTC13354_00314(miaB)
ANE : ATCC27039_05900(miaB)
AHW : NCTC11636_00075(miaB)
AIR : NCTC12972_00578(miaB)
ASLA : NCTC11923_01518(miaB)
AVC : NCTC10951_00366(miaB)
ACAP : MANAM107_20240(miaB)
BBV : HMPREF9228_0685(miaB)
BALA : DSM104299_02928(miaB)
SBAE : DSM104329_03075(miaB)
SYY : SYNGTS_0578(sll0996)
SYT : SYNGTI_0578(sll0996)
SYS : SYNPCCN_0578(sll0996)
SYQ : SYNPCCP_0578(sll0996)
SYR : SynRCC307_0604(miaB)
SYX : SynWH7803_1755(miaB)
SYH : Syncc8109_1876(miaB)
SYNW : SynWH8103_01871(miaB)
LET : O77CONTIG1_04821(miaB)
SYP : SYNPCC7002_A1915(miaB)
SYNN : NIES970_22150(miaB)
MVZ : myaer102_21340(miaB)
PRUN : PCC7821_03211(miaB)
NSPH : BDGGKGIB_02230(miaB)
DEIN : DAAJ005_12130(miaB)
OBG : Verru16b_01097(miaB)
ABIW : Abiwalacus_09370(miaB)
LCRE : Pla8534_54720(miaB)
PBU : L21SP3_02213(miaB_2)
LKB : LPTSP3_g16990(miaB_2)
FPOL : ERS445057_01823(miaB_2)
PPIO : CE91St28_15890(miaB)
BTHO : Btheta7330_05111(miaB)
BCEL : BcellWH2_02868(miaB)
BFC : BacF7301_05075(miaB)
PCRE : NCTC12858_00203(miaB_1)
PCAG : NCTC12856_00328(miaB_1)
PSOE : CE91St14_04130(miaB)
COPR : Cop2CBH44_22720(miaB)
CFAS : Cfast33896_17580(miaB)
PMZ : HMPREF0659_A6396(miaB)
PDN : HMPREF9137_0531(miaB)
PHER : prwr041_10570(miaB)
POC : NCTC13071_00546(miaB_1)
ACOU : A5CBH24_19760(miaB)
ADA : A5CPEGH6_19410(miaB)
MCOS : GM418_06885(miaB) GM418_20460(miaB)
MBAS : ALGA_2274 ALGA_4010
SMIZ : 4412673_03660(miaB_2)
SDJ : NCTC13534_05079(miaB_2)
STHA : NCTC11429_01127(miaB_1)
MGOT : MgSA37_04325(miaB_1)
PSEZ : HME7025_01386(miaB)
FOK : KK2020170_11540(miaB)
PHG : KCTC32516_00334(miaB)
AALG : AREALGSMS7_00711(miaB)
KOS : KORDIASMS9_00441(miaB)
KAN : IMCC3317_23290(miaB)
SPON : HME9304_00975(miaB)
OHM : MSHRCOH1_07520(miaB)
CHRZ : CO230_00005 CO230_00115
CGLE : NCTC11432_03116(miaB_1)
CTAI : NCTC12078_02067(miaB_2)
CTAK : 4412677_01746(miaB_1)
CHRJ : CHRYMOREF3P_2712(miaB)
CJG : NCTC13459_02245(miaB_2)
CANT : NCTC13489_02305(miaB_2)
KFA : Q73A0000_13110(miaB)
SMUM : SMML_v1c01270(miaA)
PTAN : CRYO30217_00315(miaB)
PROC : Ptc2401_02059(miaB)
MSCH : N508_000302(miaB_2)
ALAM : RT761_02506(miaB_2)
» show all
Taxonomy
Reference
Authors
Pierrel F, Bjork GR, Fontecave M, Atta M
Title
Enzymatic modification of tRNAs: MiaB is an iron-sulfur protein.
Journal
Sequence
Reference
Authors
Pierrel F, Hernandez HL, Johnson MK, Fontecave M, Atta M
Title
MiaB protein from Thermotoga maritima. Characterization of an extremely thermophilic tRNA-methylthiotransferase.
Journal
Sequence
Reference
Authors
Pierrel F, Douki T, Fontecave M, Atta M
Title
MiaB protein is a bifunctional radical-S-adenosylmethionine enzyme involved in thiolation and methylation of tRNA.
Journal
Sequence
Reference
Authors
Hernandez HL, Pierrel F, Elleingand E, Garcia-Serres R, Huynh BH, Johnson MK, Fontecave M, Atta M
Title
MiaB, a bifunctional radical-S-adenosylmethionine enzyme involved in the thiolation and methylation of tRNA, contains two essential [4Fe-4S] clusters.
Journal
Sequence
Reference
Authors
Landgraf BJ, Arcinas AJ, Lee KH, Booker SJ
Title
Identification of an intermediate methyl carrier in the radical S-adenosylmethionine methylthiotransferases RimO and MiaB.
Journal
Other DBs
ExplorEnz - The Enzyme Database: 2.8.4.3
ExPASy - ENZYME nomenclature database: 2.8.4.3
BRENDA, the Enzyme Database: 2.8.4.3