Entry
Name
tRNA 2-selenouridine synthase;
selU (gene name);
mnmH (gene name);
ybbB (gene name);
sufY (gene name)
Class
Transferases;
Transferring selenium-containing groups;
Selenotransferases
BRITE hierarchy
Sysname
geranyl diphosphate/selenophosphate:tRNA 5-methylaminomethyl-2-thiouridine34 geranyl/selenophosphatetransferase
Reaction(IUBMB)
selenophosphate + geranyl diphosphate + 5-methylaminomethyl-2-thiouridine34 in tRNA + H2O = 5-methylaminomethyl-2-selenouridine34 in tRNA + (2E)-3,7-dimethylocta-2,6-diene-1-thiol + diphosphate + phosphate (overall reaction) [RN:
R12580 ];
(1a) geranyl diphosphate + 5-methylaminomethyl-2-thiouridine34 in tRNA = 5-methylaminomethyl-2-(S-geranyl)thiouridine34 in tRNA + diphosphate [RN:
R12581 ];
(1b) selenophosphate + 5-methylaminomethyl-2-(S-geranyl)thiouridine34 in tRNA = 5-methylaminomethyl-2-(Se-phospho)selenouridine34 in tRNA + (2E)-3,7-dimethylocta-2,6-diene-1-thiol [RN:
R12582 ];
(1c) 5-methylaminomethyl-2-(Se-phospho)selenouridine34 in tRNA + H2O = 5-methylaminomethyl-2-selenouridine34 in tRNA + phosphate [RN:
R12583 ]
Reaction(KEGG)
Substrate
selenophosphate [CPD:
C05172 ];
geranyl diphosphate [CPD:
C00341 ];
5-methylaminomethyl-2-thiouridine34 in tRNA;
H2O [CPD:
C00001 ];
5-methylaminomethyl-2-(S-geranyl)thiouridine34 in tRNA;
5-methylaminomethyl-2-(Se-phospho)selenouridine34 in tRNA
Product
5-methylaminomethyl-2-selenouridine34 in tRNA;
(2E)-3,7-dimethylocta-2,6-diene-1-thiol [CPD:
C22269 ];
diphosphate [CPD:
C00013 ];
phosphate [CPD:
C00009 ];
5-methylaminomethyl-2-(S-geranyl)thiouridine34 in tRNA;
5-methylaminomethyl-2-(Se-phospho)selenouridine34 in tRNA
Comment
This bacterial enzyme converts 5-methylaminomethyl-2-uridine and 5-carboxymethylaminomethyl-2-uridine to the respective selenouridine forms in a two-step process that involves geranylation and subsequent phosphoselenation of the resulting geranylated intermediates. The resultant seleno-phosphorylated uridine intermediates further react with a water molecule to release a phosphate anion and 2-selenouridine tRNA. The enzyme contains a rhodanese domain.
History
EC 2.9.1.3 created 2020
Orthology
K06917 tRNA 2-selenouridine synthase
Genes
ECOO : ECRM13514_0333(ybbB)
ECOH : ECRM13516_0492(ybbB)
ECW : EcE24377A_0540(selU)
EDJ : ECDH1ME8569_0487(ybbB)
ECOI : ECOPMV1_00491(selU)
REE : electrica_03827(selU)
RTG : NCTC13098_05678(selU)
CLAP : NCTC11466_01122(selU)
KIE : NCTC12125_02512(selU)
BAGE : BADSM9389_31140(mnmH)
AHN : NCTC12129_03739(selU)
PSHI : SAMEA2665130_2105(selU)
SMW : SMWW4_v1c27320(selU)
SFJ : SAMEA4384070_2764(selU)
SOF : NCTC11214_04161(selU)
PSX : DR96_3111(selU) DR96_3170(selU)
PHEI : NCTC12003_02035(selU)
PRJ : NCTC6933_01939(selU)
LRI : NCTC12151_01460(selU)
PAEB : NCGM1900_4931(ybbB)
PPRC : PFLCHA0_c23440(selU)
PMUD : NCTC8068_03237(selU)
PFW : PF1751_v1c19650(selU)
PPUU : PputUW4_01620(selU)
PTRT : HU722_0011120(mnmH)
PTW : TUM18999_28020(selU)
PTAE : NCTC10697_01866(selU)
MSHE : MAALD49_36020(selU)
SCAA : TUM17387_01720(selU)
TIB : THMIRHAM_10430(selU)
TZO : THMIRHAT_02900(selU)
TNI : TVNIR_2034(selU_[H])
GAI : IMCC3135_26925(selU)
HAXI : HAALTHF_18050n(selU)
ALCA : ASALC70_03701(selU_1)
AEL : NCTC12917_02081(selU)
REH : H16_A0329(h16_A0329)
PPAI : E1956_13575(mnmH) E1956_44940(mnmH)
PLG : NCTC10937_02396(selU)
LMIR : NCTC12852_01071(selU)
CABA : SBC2_64680(selU_1) SBC2_71340(selU_2)
BTRM : SAMEA390648700108(selU_1) SAMEA390648704219(selU_2)
AXX : ERS451415_04409(selU)
LIM : L103DPR2_01678(selU)
LIH : L63ED372_01270(selU)
CARE : LT85_3035 LT85_p025
URU : DSM104443_02588(selU)
UPL : DSM104440_02320(selU)
FPHO : SHINM1_000880(selU)
BVY : NCTC9239_00524(selU)
SINL : DSM14862_03098(selU)
RMM : ROSMUCSMR3_00017(selU)
AHT : ANTHELSMS3_02675(selU)
LVS : LOKVESSMR4R_02341(selU)
HCL : NCTC13205_01208(selU)
HPUL : NCTC13154_00618(selU)
CJV : MTVDSCj20_0519(ybbB)
CCOC : CCON33237_1018(ybbB)
CLR : UPTC16701_0896(ybbB)
CLM : UPTC16712_0926(ybbB)
CAMR : CAQ16704_0916(ybbB)
CHV : CHELV3228_0457(mnmH)
CPIN : CPIN18020_1161(ybbB)
GBN : GEOBRER4_26200(selU)
BFD : NCTC4823_03212(selU)
CBAR : PATL70BA_1570(selU)
TEM : JW646_12090 JW646_15225(mnmH)
MANA : MAMMFC1_03824(selU)
SYNW : SynWH8103_01207(selU)
LET : O77CONTIG1_01344(selU)
CALN : NIES2098_05150(ybbB)
PCRE : NCTC12858_01137(selU)
MUP : A0256_13275 A0256_14250
CJG : NCTC13459_03028(selU)
MFE : Mefer_1285 Mefer_1286
MVU : Metvu_1088 Metvu_1089
MFS : MFS40622_0451 MFS40622_0452
MIF : Metin_1033 Metin_1034
MJH : JH146_0440 JH146_0441
MESG : MLAUSG7_1388 MLAUSG7_1389
MESA : MLASG1_0814 MLASG1_0815
MIG : Metig_0156 Metig_0157
MMQ : MmarC5_0706 MmarC5_0707
MMX : MmarC6_1756 MmarC6_1757
MMZ : MmarC7_0146 MmarC7_0147
MMAD : MMJJ_00920(selU) MMJJ_00930
MVN : Mevan_0021 Mevan_0022
MOK : Metok_0514 Metok_0515
METF : CFE53_05845 CFE53_05850
» show all
Taxonomy
Reference
Authors
Bartos P, Maciaszek A, Rosinska A, Sochacka E, Nawrot B
Title
Transformation of a wobble 2-thiouridine to 2-selenouridine via S-geranyl-2-thiouridine as a possible cellular pathway.
Journal
Reference
Authors
Jager G, Chen P, Bjork GR
Title
Transfer RNA Bound to MnmH Protein Is Enriched with Geranylated tRNA--A Possible Intermediate in Its Selenation?
Journal
Reference
Authors
Sierant M, Leszczynska G, Sadowska K, Komar P, Radzikowska-Cieciura E, Sochacka E, Nawrot B
Title
Escherichia coli tRNA 2-selenouridine synthase (SelU) converts S2U-RNA to Se2U-RNA via S-geranylated-intermediate.
Journal
Other DBs
ExplorEnz - The Enzyme Database: 2.9.1.3
ExPASy - ENZYME nomenclature database: 2.9.1.3
BRENDA, the Enzyme Database: 2.9.1.3