2-aminobenzimidazole [CPD:C10901];
CO2 [CPD:C00011];
methanol [CPD:C00132];
N-(1H-1,3-benzodiazol-2-yl)carbamate [CPD:C22848]
Comment
The enzyme, which is inducible in the soil bacterium Nocardioides sp. (strain SG-4G), catalyses the degradation of the fungicide carbendazim. Following hydrolysis of the carbamate ester, the carbamate decarboxylates spontaneously.
Cloning and biochemical characterization of a novel carbendazim (methyl-1H-benzimidazol-2-ylcarbamate)-hydrolyzing esterase from the newly isolated Nocardioides sp. strain SG-4G and its potential for use in enzymatic bioremediation.