KEGG ENZYME: 3.1.1.76

ENZYME: 3.1.1.76

Entry

EC 3.1.1.76                 Enzyme

Name

poly(3-hydroxyoctanoate) depolymerase;
PHO depolymerase;
poly(3HO) depolymerase;
poly[(R)-hydroxyalkanoic acid] depolymerase;
poly(HA) depolymerase;
poly(HAMCL) depolymerase;
poly[(R)-3-hydroxyoctanoate] hydrolase

Class

Hydrolases;
Acting on ester bonds;
Carboxylic-ester hydrolases

Sysname

poly{oxycarbonyl[(R)-2-pentylethylene]} hydrolase

Reaction(IUBMB)

Hydrolyses the polyester poly{oxycarbonyl[(R)-2-pentylethylene]} to oligomers [RN:R05796]

Reaction(KEGG)

R05796

Comment

The main product after prolonged incubation is the dimer [3]. Besides hydrolysing polymers of 3-hydroxyoctanoic acid, the enzyme also hydrolyses other polymers derived from medium-chain-length (C6-C12) hydroxyalkanoic acids and copolymers of mixtures of these. It also hydrolyses p-nitrophenyl esters of fatty acids. Polymers of short-chain-length hydroxyalkanoic acids such as poly[(R)-3-hydroxybutanoic acid] and poly[(R)-3-hydroxypentanoic acid] are not hydrolysed.

History

EC 3.1.1.76 created 2001, modified 2005

Orthology

K22249

poly(3-hydroxyoctanoate) depolymerase

K22250

poly(3-hydroxyoctanoate) depolymerase

Genes

LYJ:	FKV23_00190

PAE:	PA5057(phaD)

PAEV:

N297_5230(phaZ)

PAEI:

N296_5230(phaZ)

PAU:	PA14_66830(phaD)

PAP:	PSPA7_5795(phaZ)

PAG:	PLES_54471(phaD)

PAF:	PAM18_5172(phaD)

PNC:	NCGM2_0454(phaD)

PAEB:

NCGM1900_5813(phaD)

PDK:	PADK2_26780

PSG:	G655_26635

PRP:	M062_26645

PAEP:

PA1S_27405

PAER:

PA1R_gp2983

PAEM:

U769_27660

PAEL:

T223_27865

PAES:

SCV20265_5729

PAEU:

BN889_05615(phaD)

PAEG:

AI22_06590

PAEC:

M802_5228(phaZ)

PAEO:

M801_5095(phaZ)

PMY:

Pmen_0529

PMK:

MDS_0567

PRE:	PCA10_52780(phaZ)

PPSE:

BN5_0413(phaZ)

PFUW:

KF707C_53060

PALC:

A0T30_01740 A0T30_01745 A0T30_04300

PCQ:	PcP3B5_05850(catD_2)

PMUI:

G4G71_00390(phaZ)

PNT:	G5B91_02600(phaZ)

PPAA:

B7D75_26250(phaZ)

PPU:	PP_5004(phaB)

PPF:

Pput_4878

PPG:	PputGB1_5054

PPW:	PputW619_0460

PPT:

PPS_4848

PPB:	PPUBIRD1_4792

PPI:

YSA_04109

PPX:	T1E_5659(phaB)

PPUH:

B479_24485

PPUT:

L483_30175

PPUN:

PP4_50710(phaZ)

PPUD:

DW66_5239

PFV:	Psefu_0409

PMON:

X969_23945

PMOT:

X970_23580

PPJ:	RK21_05516

PMOL:

CLJ08_10590(phaZ)

PALD:

LU682_027245(phaZ)

PIX:	RIN61_09765(phaZ)

PMOS:

O165_020765

PST:	PSPTO_5144(phaB)

PSYR:

N018_23800

PSP:	PSPPH_0378(phaZ)

PSAV:

PSA3335_25360

PAMG:

BKM19_003495(phaZ)

PCI:	PCH70_48440

PAVL:

BKM03_27060(phaZ)

PVD:	CFBP1590__4931(phaB)

PSYI:

MME58_01580(phaZ)

PAST:

N015_02610(phaZ)

PLIJ:

KQP88_23285(phaZ)

PFL:	PFL_0433(phaZ)

PPRC:

PFLCHA0_c04400(phaB)

PPRO:

PPC_0447

PFE:	PSF113_0412(phaZ)

PFN:	HZ99_20100

PFO:	Pfl01_0393 Pfl01_2786

PFS:	PFLU_0395(phaB)

PFC:	PflA506_0387(phaZ)

PPZ:	H045_22855

PFB:

VO64_3471

PMAN:

OU5_3070

PTV:	AA957_12105

PCG:	AXG94_17210

PVR:	PverR02_02040

PAZO:

AYR47_09155

POI:	BOP93_01820

PRX:	HRH33_02910(phaZ)

PMED:

E3Z27_01970(phaZ)

PKR:	AYO71_04720 AYO71_09960

PMUD:

NCTC8068_00403(phaB)

PTOL:

I7845_02040(phaZ)

PFW:	PF1751_v1c03720(phaB) PF1751_v1c24960

PFF:	PFLUOLIPICF706340

PFX:	A7318_01570

PEN:	PSEEN5067(phaZ)

PBA:	PSEBR_a409

PBC:	CD58_02035

PPUU:

PputUW4_00332(phaZ1) PputUW4_02301(phaZ2)

PDR:	H681_02030

PSV:	PVLB_02160

PSK:	U771_02350

PKC:	PKB_0465(phaB)

PCH:	EY04_01535

PCZ:	PCL1606_56520

PCP:	JM49_28175

PCHP:

C4K32_0445

PALK:

PSAKL28_48970

PSW:	LK03_03710

PSES:

PSCI_2219

PSEM:

TO66_02110

PSEC:

CCOS191_0483(phaB)

PSOS:

POS17_0424

PFK:	PFAS1_09195

PANR:

A7J50_0385

PSET:

THL1_5319

PSIL:

PMA3_01420

PYM:	AK972_5522

PKE:	DLD99_02265(phaZ)

PALL:

UYA_02425

PUM:	HGP31_02220(phaZ) HGP31_23515

POJ:	PtoMrB4_52200(phaD)

PGY:	AWU82_21810(phaZ)

PLAL:

FXN65_25880(phaZ)

PSEJ:

HNQ25_21875(phaZ)

PVK:	EPZ47_01975(phaZ)

PEZ:	HWQ56_02265(phaZ) HWQ56_11915

PBZ:	GN234_16070(phaZ)

PKH:	JLK41_02530(phaZ)

PTY:	JWV26_13290(phaZ)

PMAO:

PMYSY11_4140(phaZ)

PATA:

JWU58_25445(phaZ)

PDW:	BV82_2659(phaZ)

PZE:	HU754_003075(phaZ)

PMOE:

HV782_003005(phaZ)

PCAM:

HNE05_15790(phaZ) HNE05_18180 HNE05_18185

PSEP:

C4K39_0436

PPII:

QL104_02210(phaZ)

PVW:	HU752_002965(phaZ)

PTRT:

HU722_0002890(phaZ)

PXA:	KSS93_04090(phaZ)

PSAM:

HU731_018025(phaZ)

PTZ:	HU718_002990(phaZ)

PQI:	KH389_01890(phaZ)

PSHH:

HU773_002690(phaZ)

PXN:	HU772_022870(phaZ)

PHV:	HU739_015615(phaZ)

PPRG:

HU725_021040(phaZ)

PANH:

HU763_022445(phaZ)

PASG:

KSS96_02930(phaZ)

PFAK:

KSS94_24880(phaZ)

PMUY:

KSS95_05005(phaZ)

PMAM:

KSS90_23385(phaZ)

PAZE:

KSS91_02455(phaZ)

PALV:

KSS97_03090(phaZ)

PORY:

EJA05_26030(phaZ)

PWZ:	J7655_02685(phaZ)

PTK:	EXN22_03455(phaZ)

PTW:	TUM18999_56260(phaD) TUM18999_61040

PZA:	HU749_003040(phaZ)

PIE:	HU724_002830(phaZ) HU724_013805

PWY:	HU734_002920(phaZ)

PGF:	J0G10_03020(phaZ)

PCAV:

D3880_20575(phaZ) D3880_22405

PJU:	L1P09_23185(phaZ)

PHF:	NLY38_02640(phaZ)

PIZ:	LAB08_R03570(phaZ)

PPEG:

KUA23_02020(phaZ)

PSII:

NF676_02635(phaZ)

PSIH:

LOY51_24825(phaZ)

PCAS:

LOY40_02215(phaZ)

PFIT:

KJY40_02935(phaZ)

PASI:

LG197_02715(phaZ)

PKM:	PZ739_25715(phaZ)

PNB:	NK667_25215(phaZ)

PSOA:

PSm6_15890(phaD) PSm6_19760

PTAI:

ICN73_08705(phaZ)

PKK:	QQ992_25965(phaZ)

PKJ:	Q1W70_08375(phaZ)

PHYG:

JTY93_01790(phaZ)

PHOM:

KJF94_28500(phaZ)

PCHE:

QYM18_10275(phaZ)

PCUC:

PSH97_01835(phaZ)

PTRL:

OU419_02450(phaZ)

PPAE:

LDL65_13245(phaZ)

PPAO:

K3169_02785(phaZ)

PRHZ:

CRX69_01270(phaZ)

PSJY:

AA098_01945

HPEG:

EAO82_03255(phaZ)

HAES:

LO767_14995(phaZ)

HARR:

HV822_03935 HV822_07260(phaZ)

PPHA:

BVH74_03620 BVH74_14985

SOK:	D0B54_16145

PLG:	NCTC10937_00592(phaZ)

RPEL:

N7U68_16035

SECH:

B18_10555

CCRO:

CMC5_061410

MRM:	A7982_10378

LLU:	AKJ09_11165

NANN:

O0S08_32865

PCAY:

FRD00_23995

BBA:

Bd3709

BBAT:

Bdt_3599

BBAC:

EP01_11030

BDC:	DOE51_18330

PARH:

I5S86_03190(phaZ)

RANT:

RHODO2019_14565

» show all

Reference

1 [PMID:11217416]

Authors

Jendrossek D.

Title

Microbial degradation of polyesters.

Journal

Adv Biochem Eng Biotechnol 71:293-325 (2001)
DOI:10.1007/3-540-40021-4_10

Reference

2 [PMID:10506180]

Authors

Garcia B, Olivera ER, Minambres B, Fernandez-Valverde M, Canedo LM, Prieto MA, Garcia JL, Martinez M, Luengo JM

Title

Novel biodegradable aromatic plastics from a bacterial source. Genetic and biochemical studies on a route of the phenylacetyl-coa catabolon.

Journal

J Biol Chem 274:29228-41 (1999)
DOI:10.1074/jbc.274.41.29228

Sequence

[ag:AAF02531]

Reference

3 [PMID:8476295]

Authors

Schirmer A, Jendrossek D, Schlegel HG

Title

Degradation of poly(3-hydroxyoctanoic acid) [P(3HO)] by bacteria: purification and properties of a P(3HO) depolymerase from Pseudomonas fluorescens GK13.

Journal

Appl Environ Microbiol 59:1220-7 (1993)
DOI:10.1128/AEM.59.4.1220-1227.1993

Other DBs

ExplorEnz - The Enzyme Database:

3.1.1.76

IUBMB Enzyme Nomenclature:

3.1.1.76

ExPASy - ENZYME nomenclature database:

3.1.1.76

BRENDA, the Enzyme Database:

3.1.1.76

DBGET integrated database retrieval system