Entry
Name
5' to 3' exodeoxyribonuclease (nucleoside 3'-phosphate-forming);
Cas4;
5' to 3' single stranded DNA exonuclease
Class
Hydrolases;
Acting on ester bonds;
Exodeoxyribonucleases producing 3'-phosphomonoesters
BRITE hierarchy
Reaction(IUBMB)
exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 3'-phosphates
Comment
Preference for single-stranded DNA. The enzyme from the archaeon Sulfolobus solfataricus contains a [4Fe-4S] cluster and requires a divalent metal cation, such as Mg2+ or Mn2+, for activity.
History
EC 3.1.12.1 created 2014
Orthology
K07464 CRISPR-associated exonuclease Cas4
Genes
AACN : AANUM_1922 AANUM_2197
AACT : ACT75_00735 ACT75_09830
BTRA : F544_14450 F544_8230
MOI : MOVS_00225 MOVS_04305
MBL : AAX09_00215 AAX09_03725
MAH : MEALZ_1350 MEALZ_2158
FTX : AW25_1261(cas4) AW25_606
FTD : AS84_1115 AS84_1782(cas4)
FTY : CH70_1284(cas4) CH70_545
FHI : FSC454_04740 FSC454_07205
TLO : J9253_05000(cas4) J9253_05495
TWN : L2Y54_13315 L2Y54_13425(cas4)
TDU : QJT80_01055(cas4) QJT80_07790
MPUR : MARPU_05995 MARPU_12865
TWG : Thiowin_01397(cas4-cas1)
ADK : Alide2_3280 Alide2_4785
DOE : DENOEST_P0016 DENOEST_P0115
SPLB : SFPGR_03320 SFPGR_27050
BRK : CWS35_37225 CWS35_38645
PPHR : APZ00_15355 APZ00_20155
CAMP : CFT03427_0661(cas4)
CCOO : ATE51_02484 ATE51_02910
CAMZ : CVIC12175_1285(cas4)
SMUL : SMUL_1146 SMUL_1834
ALAN : ALANTH_1108 ALANTH_1546(cas4)
DCB : C3Y92_03750 C3Y92_07105(cas4)
DTO : TOL2_C09640 TOL2_C29580(cas4)
DOV : DSCO28_05320 DSCO28_26950
DMM : dnm_042510 dnm_098410(cas4-1) dnm_098850(cas4-2)
CCRO : CMC5_026610 CMC5_084880
GTM : GT3921_06865 GT3921_09080
GWC : GWCH70_0309 GWCH70_2004
GMC : GY4MC1_1474 GY4MC1_3448
GTH : Geoth_1567 Geoth_3540
LMAC : I6G82_06575(cas4) I6G82_06640(cas4)
CTHU : HUR95_05440(cas4) HUR95_05580(cas4)
HYI : K2M58_09200 K2M58_09270
PUK : PU629_14155 PU629_19865(cas4)
PMAE : LMZ02_07015(cas4) LMZ02_07170(cas4) LMZ02_28450
ACYC : JI721_06415(cas4) JI721_09165(cas4)
KYR : CVV65_13515(cas4) CVV65_13665(cas4) CVV65_13875(cas4)
NTR : B0W44_04390 B0W44_10135
LFB : C1X05_08115(cas4) C1X05_10680(cas4)
SCP : HMPREF0833_10399(cas4)
SCAI : NCTC12191_01187(cas4)
CTET : BN906_01496 BN906_01618
CACE : CACET_c10530(cas4) CACET_c22240(cas42)
CFM : BJL90_11240 BJL90_17430
CCOH : SAMEA4530647_0927 SAMEA4530647_0996
CFER : D4Z93_06495(cas4) D4Z93_06750
CRW : CROST_006990 CROST_016970
CAUN : CLAUR_022920 CLAUR_033840
CTX : Clo1313_0765 Clo1313_2971
CCL : Clocl_2164 Clocl_2617
RBP : B6259_05965 B6259_07235
CTHD : CDO33_11175(cas4) CDO33_18940(cas4)
RGV : NQ502_04785(cas4) NQ502_06880(cas4)
CLF : GJQ69_03240 GJQ69_04580(cas4)
CARL : PXC00_03395(cas4) PXC00_08740(cas4)
SWO : Swol_0479 Swol_1804 Swol_2517
DCA : Desca_0538 Desca_0733
DRU : Desru_1407 Desru_2996
DAE : Dtox_0918 Dtox_3001 Dtox_3647 Dtox_4287
DKU : Desku_0456 Desku_0606 Desku_2969
AACX : DEACI_3643 DEACI_4263
TJR : TherJR_1886 TherJR_2490
DGI : Desgi_0547 Desgi_0810 Desgi_4164
ACAC : EYQ97_04650(cas4) EYQ97_15655(cas4)
ACEL : acsn021_19180 acsn021_28990
WHJ : H9Q79_00980(cas4) H9Q79_06610(cas4)
FAA : HMPREF0389_01167(cas4)
TEX : Teth514_1015 Teth514_2318
TPD : Teth39_0133 Teth39_0531
TMT : Tmath_0023 Tmath_1785 Tmath_2185
TBO : Thebr_0139 Thebr_0546
TWI : Thewi_2014 Thewi_2619
TKI : TKV_c18290 TKV_c23610(cas4) TKV_c24300(cas4)
TPZ : Tph_c18960(cas4) Tph_c24400(cas4)
GEK : kuro4_09980 kuro4_10060
TTM : Tthe_0574 Tthe_2613 Tthe_2699
TTO : Thethe_00571 Thethe_02657
TXY : Thexy_0460 Thexy_2275 Thexy_2351
AAUT : ACETAC_00755(cas4) ACETAC_01095(cas4) ACETAC_07900(cas4)
MTHO : MOTHE_c04310 MOTHE_c17390
MTHZ : MOTHA_c05210 MOTHA_c18220
NCD : ACONDI_00235 ACONDI_00540(addA_1)
CSC : Csac_0064 Csac_0319 Csac_2396
COB : COB47_0119 COB47_2271
CHD : Calhy_2490 Calhy_2543
CKI : Calkr_2418 Calkr_2532 Calkr_2576
CLC : Calla_2393 Calla_2440
CKN : Calkro_2440 Calkro_2487
CCHA : ELD05_00420(cas4) ELD05_12620(cas4)
CDIA : CaldiYA01_22810(cas4) CaldiYA01_23500
CNAG : OTJ99_000030(cas4) OTJ99_002359(cas4)
CMOR : OTK00_000043(cas4) OTK00_002272(cas4)
CDAN : SOJ16_000123(cas4) SOJ16_002679(cas4)
SPOA : EQM13_09790(cas4) EQM13_09845(cas4) EQM13_12570(cas4)
STED : SPTER_01830 SPTER_37200 SPTER_37890
SLE : sle_60920(sle_60920)
RDN : HMPREF0733_12108(cas4)
THAO : NI17_010220 NI17_010515
ACAD : UA74_13790 UA74_15825
ACTA : C1701_01365 C1701_23050(cas4)
BBV : HMPREF9228_1446(cas4)
RRD : RradSPS_1081 RradSPS_2774
HHG : XM38_026720(cas4-cas1)
CHON : NIES4102_19070(cas4)
MVZ : myaer102_06590(cas4)
CYH : Cyan8802_0522 Cyan8802_4560
PPSU : NO713_00174(cas4-cas1)
PRUN : PCC7821_01691(cas4-cas1)
OXY : HCG48_04120(cas4) HCG48_04475(cas4)
PYH : NEA10_00690(cas4) NEA10_18775(cas4)
MIC : Mic7113_3362 Mic7113_6624
ACY : Anacy_1506 Anacy_5669
AWA : AA650_15685 AA650_16320
HBQ : QI031_02110(cas4) QI031_14635(cas4)
CALO : Cal7507_1246 Cal7507_2969
DOU : BMF77_04925 BMF77_pa00008
DHT : NG743_01675(cas4) NG743_07455(cas4) NG743_22165(cas4)
RRS : RoseRS_1872 RoseRS_2603 RoseRS_4331
CHL : Chy400_3198 Chy400_3857
HAU : Haur_2233 Haur_2838 Haur_5083
ATM : ANT_06250 ANT_12240 ANT_28390
PSUB : Pelsub_P0163 Pelsub_P2276
ABAO : BEQ56_02700 BEQ56_02870
CAP : CLDAP_10950 CLDAP_15870
PBF : CFX0092_A0971 CFX0092_A2437
THUG : KNN16_01235(cas4) KNN16_05830
TTS : Ththe16_2094 Ththe16_2157
TBC : A0O31_02303 A0O31_02384
MSV : Mesil_3372 Mesil_3507
MHD : Marky_0278 Marky_2069
MFF : MFFC18_51550(cas4-cas1_2)
PLH : VT85_22580 VT85_26210
ACAF : CA12_25130(cas4-cas1)
PBOR : BSF38_04788 BSF38_20033
KST : KSMBR1_0475 KSMBR1_2759
BPIT : BPIT_07820 BPIT_09880 BPIT_31710
JET : L3J17_07090(cas4) L3J17_09395(cas4) L3J17_14315
LSZ : JCM16776_0966 JCM16776_1138(cas4)
LTE : JMUB4039_1029(cas4) JMUB4039_1417(cas4)
CPOR : BED41_02085 BED41_06040
AMO : Anamo_1600 Anamo_1666
PCRE : NCTC12858_00736 NCTC12858_01561
PEND : LA319_01650(cas4) LA319_02315(cas4)
PET : PEIBARAKI_4560 PEIBARAKI_5972
BLQ : L21SP5_03446 L21SP5_03449
SPIR : CWM47_09900(cas4) CWM47_30815(cas4) CWM47_35735(cas4)
SFOL : H3H32_16105(cas4) H3H32_18470(cas4)
RUP : DTQ70_09620(cas4) DTQ70_30475(cas4)
FMM : LVD15_04265(cas4) LVD15_17725(cas4)
HHO : HydHO_0401 HydHO_1172
HYS : HydSN_0412 HydSN_1201
PMX : PERMA_1340(cas_3) PERMA_A0049
TTA : Theth_1161 Theth_1690
PHY : AJ81_05775 AJ81_06170
OCY : OSSY52_01160 OSSY52_05420
MPG : Theba_1619 Theba_2306
DTH : DICTH_0063(cas4_1) DICTH_0163(cas4_2)
TYE : THEYE_A1100(cas4_1) THEYE_A2037(cas4_2)
BIH : BIP78_0910 BIP78_0923
MWO : MWSIV6_1476(cas4) MWSIV6_1625
MTEE : MTTB_01940 MTTB_03310
METE : tca_01042 tca_01190
METZ : METMT2_1106 METMT2_1236
METK : FVF72_05405 FVF72_06165(cas4)
MTHM : FZP57_07055(cas4) FZP57_07955
METJ : FZP68_02830 FZP68_03615(cas4)
MEW : MSWAN_1413 MSWAN_2147
MCUB : MCBB_1317(cas4) MCBB_2085(cas4-cas1)
METT : CIT01_02270(cas4) CIT01_04120(cas4)
MEME : HYG87_05315(cas4) HYG87_09540(cas4)
MFEG : GCM10025860_01700 GCM10025860_03880
AFU : AF_1877 AF_2181 AF_2436
AFG : AFULGI_00006220 AFULGI_00021370 AFULGI_00024520 AFULGI_00027010
APO : Arcpr_1430 Arcpr_1431
AVE : Arcve_0780 Arcve_1507
AST : Asulf_00612 Asulf_00665 Asulf_01245
FPL : Ferp_0803 Ferp_1317 Ferp_1857
GAC : GACE_0918 GACE_1635 GACE_2143
PHO : PH0175(PH0175) PH1747(PH1747)
PYA : PYCH_01690 PYCH_09590 PYCH_14580
PYS : Py04_0110 Py04_0793 Py04_1675
PYC : TQ32_03900 TQ32_06470 TQ32_08015
TSI : TSIB_1491 TSIB_1493 TSIB_1663
TBA : TERMP_00477 TERMP_00793
THE : GQS_00565 GQS_01195 GQS_03650 GQS_03660
THA : TAM4_2046 TAM4_2058 TAM4_596
THM : CL1_0380 CL1_1812 CL1_1937
TEU : TEU_06675 TEU_06720 TEU_10700
TGY : X802_06985 X802_10185 X802_10235
TCH : CHITON_0203 CHITON_1032 CHITON_2032
TPEP : A0127_00210 A0127_03565
TPIE : A7C91_07600 A7C91_09870
TCE : A3L02_02990 A3L02_08290
TBS : A3L01_02275 A3L01_04495
THH : CDI07_02230 CDI07_02240(cas4) CDI07_04410(cas4)
TSL : A3L11_06100 A3L11_06160 A3L11_08600 A3L11_09330
TTD : A3L14_03735 A3L14_06445 A3L14_11070
TPRF : A3L09_04995 A3L09_05940 A3L09_05990
TPAF : A3L08_04700 A3L08_07415
THY : A3L12_06400 A3L12_07830
TIC : FH039_04660(cas4) FH039_04670 FH039_08755(cas4)
TCQ : TIRI35C_0679 TIRI35C_0681(cas) TIRI35C_1156 TIRI35C_1321
THEM : FPV09_00930(cas4) FPV09_04660(cas4) FPV09_04670
THEI : K1720_00480 K1720_00490(cas4) K1720_00960(cas4)
PPAC : PAP_07715 PAP_07950
MBA : Mbar_A0302 Mbar_A0553
MBY : MSBRM_2643 MSBRM_3120
MBW : MSBRW_0451 MSBRW_0694
MBAR : MSBR2_2602 MSBR2_3078
MBAK : MSBR3_2822 MSBR3_3072
MMAZ : MmTuc01_0576 MmTuc01_0899 MmTuc01_0901
MMJ : MSMAS_0432 MSMAS_0754
MMAC : MSMAC_0451 MSMAC_0741
MVC : MSVAZ_0606 MSVAZ_2903
MEK : MSKOL_0587 MSKOL_2245 MSKOL_2876
MLS : MSLAZ_0522 MSLAZ_2294
METM : MSMTP_0477 MSMTP_2123 MSMTP_2827
MEF : MSWH1_0356 MSWH1_1808 MSWH1_2424
MEQ : MSWHS_0368 MSWHS_2622
MSJ : MSSAC_3488 MSSAC_3774
MSZ : MSSIH_3014 MSSIH_3312
MSW : MSSIT_3072 MSSIT_3381
MTHR : MSTHT_1493 MSTHT_2493
MTHE : MSTHC_0789 MSTHC_1796
MHOR : MSHOH_0520 MSHOH_0853
MFZ : AOB57_002960(cas4) AOB57_006555
MELO : J7W08_04875(cas4) J7W08_10835
MPOT : BKM01_05630 BKM01_07095
MZI : HWN40_00675 HWN40_10800(cas4)
MHZ : Metho_1141 Metho_2377
MTP : Mthe_0637 Mthe_0728 Mthe_0737 Mthe_1558
MCJ : MCON_0543 MCON_3269(cas4) MCON_3376(cas4)
MRTJ : KHC33_08655 KHC33_14000
MCHK : MchiMG62_05660 MchiMG62_24740
MRC : R6Y96_04350(cas4) R6Y96_09650
MEFW : F1737_02560 F1737_08140(cas4)
MFO : Metfor_0966 Metfor_2147 Metfor_2733
MEZ : Mtc_1177(cas4) Mtc_1208
HNO : LT974_05985 LT974_16425
SALR : FQU85_00525(cas4) FQU85_05080
HALR : EFA46_012400(cas4) EFA46_012525(cas4) EFA46_015030(cas4)
HDO : MUK72_06340(cas4) MUK72_19040(cas4)
SAIM : K0C01_05180(cas4) K0C01_11505
HALJ : G9465_03315 G9465_24885(cas4)
HSIN : KDQ40_14135 KDQ40_18600(cas4)
HALA : Hrd1104_10675(cas4) Hrd1104_12150
HALZ : E5139_01460 E5139_07615
HALL : LC1Hm_0077 LC1Hm_2184(cas4)
HRR : HZS55_02690 HZS55_09725(cas4)
HPEL : HZS54_02990 HZS54_10160
HAAD : MW046_01620 MW046_19120(cas4) MW046_19240(cas4)
HWC : Hqrw_1376(cas4A) Hqrw_2363(cas4B)
HLA : Hlac_0590 Hlac_3328 Hlac_3577
SRUB : C2R22_15700(cas4) C2R22_18820
HAE : halTADL_1357 halTADL_2038 halTADL_2435
HDF : AArcSl_0554(cas4) AArcSl_1509
HAH : Halar_0917 Halar_3232
NPL : FGF80_00160 FGF80_18585
HALY : HYG82_12665(cas4) HYG82_13480
HJT : DVR14_09560 DVR14_24920(cas4)
NAN : AArc1_0119 AArc1_1155
MEAE : QEN48_02415(cas4) QEN48_02535(cas4)
ABI : Aboo_0305 Aboo_1001 Aboo_1020 Aboo_1310
ACF : AciM339_0759 AciM339_1512
APE : APE_0373 APE_1239.1(cas4)
ACJ : ACAM_0276 ACAM_0344 ACAM_0785(cas4)
DFD : Desfe_1015 Desfe_1099
DMU : Desmu_0121 Desmu_0992
THG : TCELL_0595 TCELL_1045
PFM : Pyrfu_0494 Pyrfu_0578
PDL : Pyrde_0312 Pyrde_1350 Pyrde_1677
PABI : PABY_12390 PABY_16740(cas4)
PARE : PYJP_03400(cas4) PYJP_06140
STO : STK_00220(cas4) STK_23310(cas4) STK_25010(cas4) STK_26350(cas4)
SOH : D1869_12795(cas4) D1869_14385(cas4) D1869_14570(cas4)
SSO : SSO0001 SSO1392 SSO1449
SOL : Ssol_0985 Ssol_2270 Ssol_2291
SSOA : SULA_1018(cas4) SULA_2304(cas4) SULA_2322(cas4)
SSOL : SULB_1020(cas4) SULB_2305(cas4) SULB_2323(cas4)
SSOF : SULC_1019(cas4) SULC_2302(cas4) SULC_2320(cas4)
SSHI : J5U23_00249 J5U23_01679 J5U23_01723 J5U23_02140
SCAS : SACC_15550 SACC_17230 SACC_26540 SACC_31870
SACS : SUSAZ_01385 SUSAZ_10240
SIS : LS215_0156 LS215_0723 LS215_1070 LS215_1980 LS215_2278
SIA : M1425_0872 M1425_0895 M1425_1827 M1425_2113
SIM : M1627_0937 M1627_0963 M1627_2195
SID : M164_0616 M164_0892 M164_2119
SIY : YG5714_0546 YG5714_0755 YG5714_2242
SIN : YN1551_0674 YN1551_2119 YN1551_2276
SII : LD85_0628 LD85_0941 LD85_2382
SIH : SiH_0412 SiH_0772 SiH_2061
SIR : SiRe_0763 SiRe_1245 SiRe_1994
SIC : SiL_0394 SiL_0590 SiL_1967
SULA : BFU36_05840 BFU36_13355
SULE : GFS03_04760(cas4) GFS03_04920(cas4) GFS03_11540(cas4)
SULL : EWF20_00655(cas4) EWF20_13855(cas4)
MEMJ : MJ1HA_0460 MJ1HA_1283 MJ1HA_1956
MHK : DFR87_01780(cas4) DFR87_04210 DFR87_12950(cas4)
MPRU : DFR88_00770(cas4) DFR88_04780
MTEN : GWK48_07095 GWK48_10335(cas4) GWK48_10490(cas4)
MJN : MjAS7_1038 MjAS7_1997
AHO : Ahos_0358 Ahos_1052 Ahos_1751
AMAN : B6F84_00100 B6F84_09330
ABRI : DFR85_05775 DFR85_06340(cas4) DFR85_07485(cas4) DFR85_12150(cas4) DFR85_13130(cas4)
ASUL : DFR86_08015(cas4) DFR86_11520(cas4)
AAMB : D1866_00125(cas4) D1866_08995(cas4)
ACIH : HS5_16450 HS5_24440
SACD : HS1genome_1074 HS1genome_1743 HS1genome_1751
CSTY : KN1_07130 KN1_10520 KN1_13810
STEP : IC006_0544 IC006_2051 IC006_2297
SMET : RQ359_001243 RQ359_002176
PAI : PAE0079 PAE0198 PAE1763
PYR : P186_0087 P186_0902 P186_0954
PYW : PYWP30_00256 PYWP30_00789
THB : N186_03615 N186_07740
THF : MA03_02875 MA03_04855
THEL : IG193_02990 IG193_08730(cas4)
FFO : FFONT_0069 FFONT_1307
NGA : Ngar_c03800(cas4) Ngar_c26280
NVN : NVIE_017300(cas4-1) NVIE_025790(cas4-2) NVIE_027860
NCV : NCAV_0034 NCAV_0674 NCAV_1780(cas4)
» show all
Taxonomy
Reference
Authors
Zhang J, Kasciukovic T, White MF
Title
The CRISPR associated protein Cas4 Is a 5' to 3' DNA exonuclease with an iron-sulfur cluster.
Journal
Sequence
Reference
Authors
Lemak S, Beloglazova N, Nocek B, Skarina T, Flick R, Brown G, Popovic A, Joachimiak A, Savchenko A, Yakunin AF
Title
Toroidal structure and DNA cleavage by the CRISPR-associated [4Fe-4S] cluster containing Cas4 nuclease SSO0001 from Sulfolobus solfataricus.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.1.12.1
ExPASy - ENZYME nomenclature database: 3.1.12.1