KEGG ENZYME: 3.1.2.30

ENZYME: 3.1.2.30

Entry

EC 3.1.2.30                 Enzyme

Name

(3S)-malyl-CoA thioesterase;
mcl2 (gene name)

Class

Hydrolases;
Acting on ester bonds;
Thioester hydrolases

Sysname

(S)-malyl-CoA hydrolase

Reaction(IUBMB)

(S)-malyl-CoA + H2O = (S)-malate + CoA [RN:R10612]

Reaction(KEGG)

R10612

Substrate

(S)-malyl-CoA [CPD:C04348];
H2O [CPD:C00001]

Product

(S)-malate [CPD:C00149];
CoA [CPD:C00010]

Comment

Stimulated by Mg2+ or Mn2+. The enzyme has no activity with (2R,3S)-2-methylmalyl-CoA (cf. EC 4.1.3.24, malyl-CoA lyase) or other CoA esters.

History

EC 3.1.2.30 created 2014

Pathway

ec00630

Glyoxylate and dicarboxylate metabolism

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K14451

(3S)-malyl-CoA thioesterase

K19282

bifunctional (S)-malyl-CoA lyase/thioesterase

Genes

PLEI:

Q9312_08215

BOJ:	CBF45_15340

KGY:	EHF36_15360

BRD:	JL11_15545

BRG:	A4249_01015

BVC:	CEP68_03750

BREV:

E7T10_13660

BVY:	NCTC9239_00695(mcl2)

AEX:	Astex_0375

SIL:	SPO0352(citE)

SIT:	TM1040_3520

RUA:	D1823_12170

RUT:	FIU92_01320(mcl1)

RCON:

K3740_01470

JAN:

Jann_0816

RDE:	RD1_1620(citE)

RLI:	RLO149_c030880

RPON:

G3256_15365

DSH:

Dshi_2874

PGA:	PGA1_c03680

PGL:	PGA2_c03240

PGD:

Gal_03134

PHP:	PhaeoP97_02988

PPIC:

PhaeoP14_00285

PHQ:	D1820_14695

OAT:	OAN307_c41520

OAR:	OA238_c04250

OTM:	OSB_04760(mcl2)

OCT:	FTO60_02985

LMD:	METH_03315

LEJ:	ETW24_04120

LAQU:

R2C4_13635

LCAE:

K3721_03435

RED:	roselon_01727

CID:

P73_3868

CMAR:

IMCC12053_3020

CEH:	CEW89_10100

MALG:

MALG_02745

CON:	TQ29_02440

YPAC:

CEW88_11165

YAN:	AYJ57_04550

TPRO:

Ga0080559_TMP1136

PABY:

Ga0080574_TMP3142

SUAM:

BOO69_13680

SULZ:

C1J03_18190

SULI:

C1J05_18110

SULD:

B5M07_04980

SPOT:

G6548_04145

SMED:

JNX03_18050

SINL:

DSM14862_02486(mcl2)

SFAV:

PL335_11845

SUAL:

KDD17_02140

SDUB:

R1T39_14805

SPSE:

SULPSESMR1_00394(mcl2)

TOM:	BWR18_01025

RMM:	ROSMUCSMR3_02505(mcl2)

ROK:	RAK1035_0677

RID:	RIdsm_04538(mcl2)

ROM:	EI983_02900

ROH:	FIU89_19180(mcl1)

RPEL:

N7U68_08430

AHT:	ANTHELSMS3_02127(mcl2)

SAGU:

CDO87_04390

THAA:

CFI11_04355

OCD:	FHY55_05855

ROT:	FIV09_01380(mcl1)

RMH:	LVO79_01055

MALU:

KU6B_41840(mcl2)

TGL:	HFZ77_08415

HAT:	RC74_09365

PALX:

GQA70_04790

LSAL:

KBK07_01415

LALG:

LentiSH36_00609

TRY:	QF118_18685

PLCG:

RVY76_03675

MARU:

FIU81_04115(mcl1)

RMAI:

MACH21_05280(mcl2)

RSP:

RSP_0970

RSH:	Rsph17029_2630

RSQ:	Rsph17025_0082

RSK:	RSKD131_2362

CAZT:

LV780_00415

RCP:	RCAP_rcc00727(citE1)

RHP:	LPB142_03415

RBL:	B6K69_07625

PDE:

Pden_0563

PAMI:

JCM7686_2574

PYE:	A6J80_13300

PCON:

B0A89_09550

PZH:	CX676_05535

PARO:

CUV01_17720

PAMN:

JCM7685_2545

PARS:

DRW48_09375

PARR:

EOJ32_08540

PAAK:

FIU66_10030

PKD:	F8A10_02165

PPAN:

ESD82_12320

PLIA:

E4191_08475

PSAN:

HGN31_04095

PMAU:

CP157_01939(mcl2)

PMEH:

JWJ88_01995

PSEW:

JHW44_08040

PSAP:

JHX88_13540

PFIS:

JHX87_05220

PAEX:

JHW48_10985

PSTL:

JHW45_07345

PALP:

JHW40_16600

PVER:

E3U25_22180

PFEO:

E3U26_10440

PTP:	RCA23_c14020(citE)

RSU:	NHU_00724(citE)

RHM:	B5V46_04475

RHC:

RGUI_0622

RMB:	K529_020740

TSCO:

R1T40_12735

DAA:	AKL17_3637

DON:	BSK21_11440

RBZ:	B9057_02175

THW:	BMG03_06670

TEC:	AKL02_005525

LVS:	LOKVESSMR4R_02803(mcl2)

RBG:	BG454_13655

GEH:	HYN69_13115

GFU:	KM031_13965

TAW:	EI545_06975

SALO:

EF888_18565

SEDI:

EBB79_02250

PPSO:

QPJ95_04700

HML:	HmaOT1_03960

BOO:	E2K80_01990

PSEB:

EOK75_09400

LIT:	FPZ52_01350

PPRU:

FDP22_03640

PAED:

G5B38_15490

MON:	G8E03_14605

PAMO:

BAR1_14395

POZ:	I0K15_13770

PALW:

PSAL_019030(mcl2)

PSHQ:

F3W81_20275

PPAF:

I8N54_16895

CACT:

HZ995_03660

THAS:

C6Y53_10240

HDH:	G5B40_16110

CMAG:

CBW24_13080

FAP:	GR316_00365

GCE:	KYE46_04235

NSM:	JO391_19965

ACRO:

K3J57_02795

SINR:

O5O51_12655

AALK:

LGT41_0002075

AMYL:

QBD29_06840

AMAQ:

GO499_14350

RHOC:

QTA57_01875

PBAE:

P8S53_11195

LVR:	T8T21_09445

FAQ:	G5B39_05115

RBX:	I3V23_06850

HYT:	HXX25_10655

SPHK:

SKP52_03440

SPHP:

LH20_16080

SMAG:

AN936_01440

SMAZ:

LH19_01485

STER:

AOA14_02930

SGI:	SGRAN_0950(mcl2)

SPHQ:

BWQ93_11140

SPHO:

C3E99_01130

SPHX:

E5675_17240

SINA:

KNJ79_16505

SPHU:

SPPYR_0495(mcl)

SPHI:

TS85_09155

SSAN:

NX02_12375

SPLM:

BXU08_04065

SPHC:

CVN68_04030

SLAN:

GV829_09535

SPAI:

FPZ24_11710

SARI:

H5J25_02820

SSUA:

FPZ54_04690

SBIN:

SBA_ch1_14890(citE)

SJP:	SJA_C1-26890(citE)

SINB:

SIDU_01380

SCH:	Sphch_1616

SSY:

SLG_14830

SYB:	TZ53_00395

SBD:	ATN00_05850

SPMI:

K663_04105

SPHB:

EP837_00844(citE)

SPHR:

BSY17_744

SPHT:

K426_06735

SHYD:

CJD35_07870

SYA:	A6768_15560

SCLO:

SCLO_1015050

SPYG:

YGS_C1P0058

SUFL:

FIL70_12030

SBAR:

H5V43_09595

SAMI:

SAMIE_1022050

SLAA:

EUU25_05605

SPZR:

G5C33_12750

PALG:

HFP57_06190

SPHS:

ETR14_23585

SFLV:

IC614_03365

HTM:	RZR91_08980

CPAF:

C6V80_02765

RPY:	Y013_11815

RBY:	CEJ39_22715

RHQ:	IM25_03505

GTA:	BCM27_21690

BSOL:

FSW04_20430

DDR:	Deide_20020

DMR:	Deima_2864

DPT:	Deipr_2520

DGO:	DGo_CA0363(citE)

DSW:	QR90_15895

DCH:	SY84_10370

DAB:	AUC44_01395

DPU:	SU48_13230

DWU:	DVJ83_08830

DFC:

DFI_10115

DEIN:

DAAJ005_03145

BLQ:	L21SP5_01621(mcl2)

HDL:	HALDL1_15310

HHB:	Hhub_3380(aceB)

HNO:	LT974_13710

HLU:	LT972_01030

HSU:	HLASF_2007(aceB)

HSF:	HLASA_1993(aceB)

HAHS:

HSRCO_2658(mcl2)

SALR:

FQU85_05715

HDO:	MUK72_12480

HALX:

M0R89_21245

SAIM:

K0C01_12450

HMA:	rrnAC1965(aceB)

HHI:	HAH_2476(aceB)

HHN:	HISP_12605

HAB:	SG26_04910

HTA:	BVU17_12045

HSIN:

KDQ40_00995

NPH:	NP_4430A(aceB)

NMO:	Nmlp_2357(aceB)

NHO:	HWV23_13655

NSAL:

HWV07_07395

HALI:

BV210_07645

SSAI:

N0B31_16615

HAAD:

MW046_18245

HWA:	HQ_1720A(aceB)

HWC:	Hqrw_1842(aceB)

HVO:	HVO_1983(aceB1) HVO_B0200(aceB2)

HME:	HFX_2078(aceB)

HGI:	ABY42_09665 ABY42_16600

HALE:

G3A49_07690 G3A49_12900

HLS:	KU306_02605

HLN:	SVXHx_2637(aceb) SVXHx_3054(aceb)

HBO:	Hbor_30530

HLM:	DV707_13970

SRUB:

C2R22_21540

HALG:

HUG10_03375

HALU:

HUG12_13540

HMP:	K6T50_01580

HRE:	K6T36_01385

HRM:	K6T25_15035

HACB:

Hbl1158_08495

HDF:	AArcSl_1031(aceB)

HTU:

Htur_4695

HSAL:

JMJ58_24075

NMG:	Nmag_3363(aceB)

NOU:	Natoc_4308

NAJ:	B1756_06555

NBG:	DV706_17910

NAS:	GCU68_18820

» show all

Reference

1 [PMID:20047909]

Authors

Erb TJ, Frerichs-Revermann L, Fuchs G, Alber BE

Title

The apparent malate synthase activity of Rhodobacter sphaeroides is due to two paralogous enzymes, (3S)-Malyl-coenzyme A (CoA)/{beta}-methylmalyl-CoA lyase and (3S)- Malyl-CoA thioesterase.

Journal

J Bacteriol 192:1249-58 (2010)
DOI:10.1128/JB.01267-09

Sequence

[rsp:RSP_0970]

Other DBs

ExplorEnz - The Enzyme Database:

3.1.2.30

IUBMB Enzyme Nomenclature:

3.1.2.30

ExPASy - ENZYME nomenclature database:

3.1.2.30

BRENDA, the Enzyme Database:

3.1.2.30

DBGET integrated database retrieval system