KEGG   ENZYME: 3.1.21.8
Entry
EC 3.1.21.8                 Enzyme                                 
Name
T4 deoxyribonuclease II;
T4 endonuclease II;
EndoII (ambiguous);
denA (gene name)
Class
Hydrolases;
Acting on ester bonds;
Endodeoxyribonucleases producing 5'-phosphomonoesters
Reaction(IUBMB)
Endonucleolytic nicking and cleavage of cytosine-containing double-stranded DNA.
Comment
Requires Mg2+. This phage T4 enzyme is involved in degradation of host DNA. The enzyme primarily catalyses nicking of the bottom strand of double stranded DNA between the first and second base pair to the right of a top-strand CCGC motif. Double-stranded breaks are produced 5- to 10-fold less frequently [3]. It does not cleave the T4 native DNA, which contains 5-hydroxymethylcytosine instead of cytosine.
History
EC 3.1.21.8 created 2014
Orthology
K20841  Straboviridae deoxyribonuclease II
Reference
1  [PMID:8386173]
  Authors
Carlson K, Krabbe M, Nystrom AC, Kosturko LD
  Title
DNA determinants of restriction. Bacteriophage T4 endonuclease II-dependent cleavage of plasmid DNA in vivo.
  Journal
J Biol Chem 268:8908-18 (1993)
  Sequence
[vg:1258710]
Reference
2  [PMID:9515694]
  Authors
Carlson K, Kosturko LD
  Title
Endonuclease II of coliphage T4: a recombinase disguised as a restriction endonuclease?
  Journal
Mol Microbiol 27:671-6 (1998)
DOI:10.1046/j.1365-2958.1998.00728.x
Reference
3  [PMID:10200960]
  Authors
Carlson K, Kosturko LD, Nystrom AC
  Title
Sequence-specific cleavage by bacteriophage T4 endonuclease II in vitro.
  Journal
Mol Microbiol 31:1395-405 (1999)
DOI:10.1046/j.1365-2958.1999.01281.x
Reference
4  [PMID:20156453]
  Authors
Andersson CE, Lagerback P, Carlson K
  Title
Structure of bacteriophage T4 endonuclease II mutant E118A, a tetrameric GIY-YIG enzyme.
  Journal
J Mol Biol 397:1003-16 (2010)
DOI:10.1016/j.jmb.2010.01.076
  Sequence
[vg:1258710]
Other DBs
ExplorEnz - The Enzyme Database: 3.1.21.8
IUBMB Enzyme Nomenclature: 3.1.21.8
ExPASy - ENZYME nomenclature database: 3.1.21.8
BRENDA, the Enzyme Database: 3.1.21.8

  All links  
Gene (167)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (159)   
   KEGG MGENES (7)   
Protein sequence (38)   
   UniProt (26)   
   SWISS-PROT (1)   
   RefSeq(pep) (11)   
DNA sequence (102)   
   RefSeq(nuc) (15)   
   GenBank (46)   
   EMBL (41)   
Protein domain (5)   
   InterPro (5)   
All databases (312)   

Download RDF
DBGET integrated database retrieval system