KEGG ENZYME: 3.1.4.60

ENZYME: 3.1.4.60

Entry

EC 3.1.4.60                 Enzyme

Name

pApA phosphodiesterase;
pde2 (gene name);
pApA hydrolase

Class

Hydrolases;
Acting on ester bonds;
Phosphoric-diester hydrolases

Sysname

5'-O-phosphonoadenylyl-(3'->5')-adenosine phosphohydrolase

Reaction(IUBMB)

5'-O-phosphonoadenylyl-(3'->5')-adenosine + H2O = 2 AMP [RN:R12346]

Reaction(KEGG)

R12346

Substrate

5'-O-phosphonoadenylyl-(3'->5')-adenosine [CPD:C22092];
H2O [CPD:C00001]

Product

AMP [CPD:C00020]

Comment

The enzyme, characterized from the Gram-positive bacterium Staphylococcus aureus, is a cytoplasmic protein that contains a DHH/DHHA1 domain. It can act on cyclic di-3',5'-adenylate with a much lower activity (cf. EC 3.1.4.59, cyclic-di-AMP phosphodiesterase). Activity requires Mn2+ and is inhibited by ppGpp.

History

EC 3.1.4.60 created 2019

Reference

1 [PMID:24013631]

Authors

Bai Y, Yang J, Eisele LE, Underwood AJ, Koestler BJ, Waters CM, Metzger DW, Bai G

Title

Two DHH subfamily 1 proteins in Streptococcus pneumoniae possess cyclic di-AMP phosphodiesterase activity and affect bacterial growth and virulence.

Journal

J Bacteriol 195:5123-32 (2013)
DOI:10.1128/JB.00769-13

Sequence

[spd:SPD_1153]

Reference

2 [PMID:24566626]

Authors

Ye M, Zhang JJ, Fang X, Lawlis GB, Troxell B, Zhou Y, Gomelsky M, Lou Y, Yang XF

Title

DhhP, a cyclic di-AMP phosphodiesterase of Borrelia burgdorferi, is essential for cell growth and virulence.

Journal

Infect Immun 82:1840-9 (2014)
DOI:10.1128/IAI.00030-14

Sequence

[bbu:BB_0619]

Reference

3 [PMID:26078723]

Authors

Tang Q, Luo Y, Zheng C, Yin K, Ali MK, Li X, He J

Title

Functional Analysis of a c-di-AMP-specific Phosphodiesterase MsPDE from Mycobacterium smegmatis.

Journal

Int J Biol Sci 11:813-24 (2015)
DOI:10.7150/ijbs.11797

Sequence

[msm:MSMEG_2630]

Reference

4 [PMID:26691161]

Authors

Kuipers K, Gallay C, Martinek V, Rohde M, Martinkova M, van der Beek SL, Jong WS, Venselaar H, Zomer A, Bootsma H, Veening JW, de Jonge MI

Title

Highly conserved nucleotide phosphatase essential for membrane lipid homeostasis in Streptococcus pneumoniae.

Journal

Mol Microbiol 101:12-26 (2016)
DOI:10.1111/mmi.13312

Sequence

[spn:SP_1298]

Reference

5 [PMID:27834680]

Authors

Bowman L, Zeden MS, Schuster CF, Kaever V, Grundling A

Title

New Insights into the Cyclic Di-adenosine Monophosphate (c-di-AMP) Degradation Pathway and the Requirement of the Cyclic Dinucleotide for Acid Stress Resistance in Staphylococcus aureus.

Journal

J Biol Chem 291:26970-26986 (2016)
DOI:10.1074/jbc.M116.747709

Sequence

[saa:SAUSA300_1650]

Other DBs

ExplorEnz - The Enzyme Database:

3.1.4.60

IUBMB Enzyme Nomenclature:

3.1.4.60

ExPASy - ENZYME nomenclature database:

3.1.4.60

BRENDA, the Enzyme Database:

3.1.4.60

All links

Chemical substance (3)   
   KEGG COMPOUND (3)   
Chemical reaction (2)   
   KEGG REACTION (1)   
   KEGG RCLASS (1)   
All databases (5)   

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