Entry
Name
S-sulfosulfanyl-L-cysteine sulfohydrolase;
SoxB
Class
Hydrolases;
Acting on ester bonds;
Sulfuric-ester hydrolases
BRITE hierarchy
Sysname
[SoxY protein]-S-sulfosulfanyl-L-cysteine sulfohydrolase
Reaction(IUBMB)
(1) [SoxY protein]-S-sulfosulfanyl-L-cysteine + H2O = [SoxY protein]-S-sulfanyl-L-cysteine + sulfate [RN:
R12096 ];
(2) [SoxY protein]-S-(2-sulfodisulfanyl)-L-cysteine + H2O = [SoxY protein]-S-disulfanyl-L-cysteine + sulfate [RN:
R12097 ]
Reaction(KEGG)
Substrate
[SoxY protein]-S-sulfosulfanyl-L-cysteine [CPD:
C21815 ];
H2O [CPD:
C00001 ];
[SoxY protein]-S-(2-sulfodisulfanyl)-L-cysteine [CPD:
C21899 ]
Product
[SoxY protein]-S-sulfanyl-L-cysteine [CPD:
C21898 ];
sulfate [CPD:
C00059 ];
[SoxY protein]-S-disulfanyl-L-cysteine [CPD:
C21814 ]
Comment
Contains Mn2+. The enzyme is part of the Sox enzyme system, which participates in a bacterial thiosulfate oxidation pathway that produces sulfate. It catalyses two reactions in the pathway. In both cases the enzyme hydrolyses a sulfonate moiety that is bound (either directly or via a sulfane) to a cysteine residue of a SoxY protein, releasing sulfate. The enzyme from Paracoccus pantotrophus contains a pyroglutamate (cycloglutamate) at its N-terminus.
History
EC 3.1.6.20 created 2018
Pathway
Orthology
K17224 S-sulfosulfanyl-L-cysteine sulfohydrolase
Genes
HTR : EPV75_04415 EPV75_08360
HMAR : HVMH_0780(soxB_1) HVMH_1723(soxB_2)
THIO : AYJ59_05560 AYJ59_09395
TIB : THMIRHAM_14130(soxB)
TSE : THMIRHAS_10590(soxB)
TZO : THMIRHAT_11840(soxB)
TNI : TVNIR_3254(yfkN_[H])
GAI : IMCC3135_13775(mggB)
THIC : TspCOW1_15140(soxB)
ACII : C4901_02710 C4901_13035(soxB)
ATY : A9R16_002295 A9R16_003935(soxB)
VFF : VITFI_CDS0979 VITFI_CDS2148
REH : H16_A3563(h16_A3563)
AKA : TKWG_08995 TKWG_14010
LIM : L103DPR2_01197(yfkN)
LIH : L63ED372_00551(yfkN_1)
HYR : BSY239_2850 BSY239_838(soxB)
MELA : C6568_01375 C6568_08775(soxB)
TBD : Tbd_0563 Tbd_1286 Tbd_1292 Tbd_2012
SULF : CAP31_05840 CAP31_10670 CAP31_12810
SNIV : SFSGTM_02850(soxB) SFSGTM_09690 SFSGTM_09750
BJA : bll1009(soxB) blr3515(soxB)
AOL : S58_06180 S58_44030(soxB)
BRO : BRAD285_2961 BRAD285_6992
BRK : CWS35_09080(soxB) CWS35_37710 CWS35_37800(soxB) CWS35_37875
BOT : CIT37_09255(soxB) CIT37_36640(soxB)
BRQ : CIT40_11180(soxB) CIT40_30415(soxB)
BGQ : X265_02705(soxB) X265_35000(soxB)
BGZ : XH91_21965(soxB) XH91_33350(soxB)
BSYM : CIT39_10290(soxB) CIT39_29520(soxB)
BBET : F8237_13340(soxB) F8237_34490(soxB) F8237_35435 F8237_35745
BSEP : HAP48_0012980(soxB)
BQB : J4P68_0016835(soxB) J4P68_0027355(soxB) J4P68_0034840(soxB)
BCAN : BcanWSM471_35790(soxB)
BROS : QUH67_11125(soxB) QUH67_33915(soxB)
VGO : GJW-30_1_01696(yfkN_1)
MMES : MMSR116_16040(soxB)
RLI : RLO149_c031840(soxB1)
PHP : PhaeoP97_01242(soxB2)
PPIC : PhaeoP14_01165(soxB2)
SINL : DSM14862_01880(mggB)
RMM : ROSMUCSMR3_02025(mggB)
LALG : LentiSH36_01644(soxB2)
RMAI : MACH21_17350(soxB_1)
SMUC : JL100_018865(soxB) JL100_025435(soxB)
TTB : MACH01_17120(soxB_1)
ACZ : Acaty_c2057 Acaty_c2213
ATX : GCD22_00897(mggB) GCD22_03168(nutA)
ACIY : MQE22_02650(soxB) MQE22_03580(soxB)
JDA : BW727_101019(mggB_2)
PLH : VT85_10605(yfkN_1) VT85_22465(yfkN_2)
GFO : GFO_1276(ushA) GFO_1279
AALG : AREALGSMS7_00606 AREALGSMS7_00608
PROC : Ptc2401_00854(yfkN_2)
NAY : HYG81_20180 HYG81_25155
NAS : GCU68_17325 GCU68_19000
» show all
Taxonomy
Reference
Authors
Quentmeier A, Friedrich CG
Title
The cysteine residue of the SoxY protein as the active site of protein-bound sulfur oxidation of Paracoccus pantotrophus GB17.
Journal
Reference
Authors
Friedrich CG, Rother D, Bardischewsky F, Quentmeier A, Fischer J
Title
Oxidation of reduced inorganic sulfur compounds by bacteria: emergence of a common mechanism?
Journal
Reference
Authors
Quentmeier A, Hellwig P, Bardischewsky F, Grelle G, Kraft R, Friedrich CG
Title
Sulfur oxidation in Paracoccus pantotrophus: interaction of the sulfur-binding protein SoxYZ with the dimanganese SoxB protein.
Journal
Reference
Authors
Epel B, Schafer KO, Quentmeier A, Friedrich C, Lubitz W
Title
Multifrequency EPR analysis of the dimanganese cluster of the putative sulfate thiohydrolase SoxB of Paracoccus pantotrophus.
Journal
Reference
Authors
Hensen D, Sperling D, Truper HG, Brune DC, Dahl C
Title
Thiosulphate oxidation in the phototrophic sulphur bacterium Allochromatium vinosum.
Journal
Sequence
Reference
Authors
Grabarczyk DB, Berks BC
Title
Intermediates in the Sox sulfur oxidation pathway are bound to a sulfane conjugate of the carrier protein SoxYZ.
Journal
Other DBs
ExplorEnz - The Enzyme Database: 3.1.6.20
ExPASy - ENZYME nomenclature database: 3.1.6.20