Hydrolases;
Acting on ester bonds;
Sulfuric-ester hydrolases
BRITE hierarchy

primary alkyl sulfate ester sulfohydrolase

a primary alkyl sulfate ester + H2O = an alcohol + sulfate [RN:R12797]

R12797
Reaction

primary alkyl sulfate ester;
H2O [CPD:C00001]

Sulfatase enzymes are classified as type I, in which the key catalytic residue is 3-oxo-L-alanine, type II, which are non-heme iron-dependent dioxygenases, or type III, whose catalytic domain adopts a metallo-beta-lactamase fold and binds two zinc ions as cofactors. This enzyme belongs to the type III sulfatase family. It is active against linear primary-alkyl sulfate esters, such as dodecyl sulfate, decyl sulfate, octyl sulfate, and hexyl sulfate. The enzyme from Pseudomonas aeruginosa is secreted out of the cell. The catalytic mechanism begins with activation of a water molecule by the binuclear Zn2+ cluster, resulting in a nucleophilic attack on the carbon atom. cf. EC 3.1.6.22, branched primary-alkylsulfatase, and EC 3.1.6.19, (R)-specific secondary-alkylsulfatase (type III).

EC 3.1.6.21 created 2021