KEGG ENZYME: 3.2.1.107

ENZYME: 3.2.1.107

Entry

EC 3.2.1.107                Enzyme

Name

protein-glucosylgalactosylhydroxylysine glucosidase;
PGGHG (gene name);
2-O-alpha-D-glucopyranosyl-5-O-alpha-D-galactopyranosylhydroxy-L-lysine glucohydrolase;
protein-alpha-D-glucosyl-1,2-beta-D-galactosyl-L-hydroxylysine glucohydrolase;
protein-alpha-D-glucosyl-(1->2)-beta-D-galactosyl-L-hydroxylysine glucohydrolase

Class

Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

Sysname

[collagen]-(5R)-5-O-[alpha-D-glucosyl-(1->2)-beta-D-galactosyl]-5-hydroxy-L-lysine glucohydrolase

Reaction(IUBMB)

[collagen]-(5R)-5-O-[alpha-D-glucosyl-(1->2)-beta-D-galactosyl]-5-hydroxy-L-lysine + H2O = D-glucose + [collagen]-(5R)-5-O-(beta-D-galactosyl)-5-hydroxy-L-lysine [RN:R04503]

Reaction(KEGG)

R04503

Substrate

[collagen]-(5R)-5-O-[alpha-D-glucosyl-(1->2)-beta-D-galactosyl]-5-hydroxy-L-lysine;
H2O [CPD:C00001]

Product

D-glucose [CPD:C00031];
[collagen]-(5R)-5-O-(beta-D-galactosyl)-5-hydroxy-L-lysine

Comment

The enzyme specifically hydrolyses glucose from alpha-D-glucosyl-(1->2)-beta-D-galactosyl disaccharide units that are linked to hydroxylysine residues of collagen and collagen-like proteins. Acetylation of the epsilon-amino group of the glycosylated hydroxylysine abolishes activity.

History

EC 3.2.1.107 created 1984

Orthology

K22078

protein-glucosylgalactosylhydroxylysine glucosidase

Genes

HSA:	80162(PGGHG)

PTR:	743704(PGGHG)

PPS:	100971508(PGGHG)

GGO:	109025438(PGGHG)

PON:	100454348(PGGHG)

NLE:	100601940(PGGHG)

HMH:	116466561(PGGHG)

MCC:	697199(PGGHG)

MCF:	101926478(PGGHG)

MTHB:

126936558

MNI:	105494123(PGGHG)

CSAB:

103236799(PGGHG)

CATY:

105577182(ATHL1)

PANU:

100997983(PGGHG)

TGE:	112606324(PGGHG)

MLEU:

105530810(ATHL1)

RRO:	104655560(PGGHG)

RBB:	108537993(PGGHG)

TFN:	117081588(PGGHG)

PTEH:

111520543(PGGHG)

CANG:

105503186(ATHL1)

CJC:	100385744(PGGHG)

SBQ:	101049147(ATHL1)

CIMI:

108298302(PGGHG)

CSYR:

103255720(PGGHG)

MMUR:

105874073(PGGHG)

LCAT:

123642985(PGGHG)

PCOQ:

105816546(ATHL1)

OGA:	100942793(PGGHG)

MMU:	212974(Pgghg)

MCAL:

110299030(Pgghg)

MPAH:

110325920(Pgghg)

RNO:	309103(Pgghg)

MCOC:

116103197(Pgghg)

ANU:	117697618(Pgghg)

MUN:	110553930(Pgghg)

CGE:	100757546(Pgghg)

MAUA:

101841403(Pgghg)

PROB:

127228517(Pgghg)

PLEU:

114693487(Pgghg)

MORG:

121457716(Pgghg)

MFOT:

126498799

AAMP:

119801636(Pgghg)

NGI:	103751062(Pgghg)

HGL:	101723837(Pgghg)

CPOC:

100719730(Pgghg)

CCAN:

109698993(Pgghg)

DORD:

105998608(Athl1)

DSP:	122095013(Pgghg)

PLOP:

125361271(Pgghg)

NCAR:

124960687

MMMA:

107157112(Pgghg)

OCU:

103347496

OPI:	101515994(PGGHG)

TUP:	102501509(PGGHG)

GVR:	103602145(ATHL1)

CFA:	483395(PGGHG)

CLUD:

112663545(PGGHG)

VVP:	112914422(PGGHG)

VLG:	121471379(PGGHG)

NPO:	129494305(PGGHG)

AML:	100469466(PGGHG)

UMR:	103671029(PGGHG)

UAH:	113248810(PGGHG)

UAR:	123775496(PGGHG)

ELK:

111150122

LLV:

125078262

MPUF:

101675114(ATHL1)

MNP:	132016262(PGGHG)

MLK:	131818493(PGGHG)

NVS:	122913206(PGGHG)

ORO:	101386980(ATHL1)

EJU:	114201538(PGGHG)

ZCA:	113914867(PGGHG)

MLX:	118016230(PGGHG)

NSU:	110582230(PGGHG)

LWW:	102745174(PGGHG)

FCA:	101087100(PGGHG)

PYU:	121022771(PGGHG)

PCOO:

112852086(PGGHG)

PBG:	122484191(PGGHG)

PVIV:

125146959(PGGHG)

LRUF:

124527555

PTG:	102949755(PGGHG)

PPAD:

109259816(PGGHG)

PUC:

125937748

AJU:

106987954

HHV:	120223433(PGGHG)

BTA:	511919(PGGHG)

BOM:	102276155(PGGHG)

BBUB:

102405787(PGGHG)

BBIS:

104983406(ATHL1)

CHX:	102188292(PGGHG)

OAS:	101106382(PGGHG)

BTAX:

128068852(PGGHG)

ODA:	120874199(PGGHG)

CCAD:

122430802(PGGHG)

MBEZ:

129548584(PGGHG)

SSC:	100518005(PGGHG)

CFR:	102511585(PGGHG)

CBAI:

105064430(ATHL1)

CDK:	105105739(PGGHG)

VPC:	102541806(PGGHG)

BACU:

103010340(PGGHG)

BMUS:

118899223(PGGHG)

LVE:	103071239(ATHL1)

OOR:	101279414(PGGHG)

DLE:	111170281(PGGHG)

PCAD:

102991703(PGGHG)

PSIU:

116757783(PGGHG)

NASI:

112402474(PGGHG)

ECB:	100146757(PGGHG)

EPZ:	103568056(PGGHG)

EAI:	106843884(PGGHG)

MYB:	102252209(PGGHG)

MYD:	102760826(PGGHG)

MMYO:

118649081(PGGHG)

PKL:	118712997(PGGHG)

EFUS:

103302589(PGGHG)

MNA:	107544451(ATHL1)

DRO:	112317246(PGGHG)

SHON:

118974897(PGGHG)

AJM:	119060967(PGGHG)

PDIC:

114499171(PGGHG)

PHAS:

123807371(PGGHG)

MMF:	118643163(PGGHG)

PPAM:

129073125(PGGHG)

HAI:	109378078(PGGHG)

RFQ:	117030533(PGGHG)

PALE:

102887768(PGGHG)

PGIG:

120614156(PGGHG)

PVP:	105309813(PGGHG)

RAY:	107506507(ATHL1)

MJV:	108384850(PGGHG)

TOD:	119249819(PGGHG)

SARA:

101553230(ATHL1)

SETR:

126018843(PGGHG)

LAV:	100655235(PGGHG)

TMU:

101349759

ETF:	101643584(PGGHG)

DNM:	101410876(PGGHG)

MDO:	103092300(PGGHG)

GAS:	123252860(PGGHG)

SHR:	100923289(PGGHG)

AFZ:

127539999

PCW:	110203719(PGGHG)

OAA:	100091579(PGGHG)

GGA:	422991(PGGHG)

PCOC:

116237919(PGGHG)

MGP:	100538987(PGGHG)

CJO:	107314276(PGGHG)

TPAI:

128085942(PGGHG)

LMUT:

125694964(PGGHG)

NMEL:

110401494(PGGHG)

APLA:

101791880(PGGHG)

ACYG:

106047048(PGGHG)

CATA:

118244241(PGGHG)

AFUL:

116490363(PGGHG)

TGU:	100221918(PGGHG)

LSR:	110467551(PGGHG)

SCAN:

103827054(PGGHG)

PMOA:

120500985(PGGHG)

OTC:	121334793(PGGHG)

PRUF:

121352365(PGGHG)

GFR:	102035352(PGGHG)

FAB:	101806950(PGGHG)

OMA:	130253955(PGGHG)

PHI:	102112573(ATHL1)

PMAJ:

107205256(PGGHG)

CCAE:

111930573(PGGHG)

CCW:	104684542(PGGHG)

CBRC:

103614577(PGGHG)

ETL:	114063280(PGGHG)

ZAB:	102072796(PGGHG)

ACHL:

103805928(ATHL1)

SVG:	106854807(ATHL1)

MMEA:

130581674(PGGHG)

HRT:	120754157(PGGHG)

FPG:	101919210(PGGHG)

FCH:	102051782(PGGHG)

CLV:	102089207(PGGHG)

EGZ:	104127885(ATHL1)

NNI:	104011413(ATHL1)

PCRI:

104034685(ATHL1)

PCAO:

104047290(ATHL1)

ACUN:

113481198(PGGHG)

TALA:

104365197(PGGHG)

PADL:

103925396(ATHL1)

AFOR:

103906296(PGGHG)

ACHC:

115351889(PGGHG)

HALD:

104316724(ATHL1)

HLE:	104832093(ATHL1)

AGEN:

126047544

GCL:

127016727

CCRI:

104169557(ATHL1)

MUI:	104546391(ATHL1)

BREG:

104634605(ATHL1)

FGA:	104077869(ATHL1)

GSTE:

104253621(ATHL1)

LDI:	104352058(ATHL1)

OHA:	104333131(ATHL1)

NNT:	104407864(ATHL1)

SHAB:

115607583(PGGHG)

DPUB:

104297526(ATHL1)

ACAR:

104529766(PGGHG)

CPEA:

104392123(ATHL1)

AVIT:

104277475(ATHL1)

CVF:	104282478(ATHL1)

RTD:	128908404(PGGHG)

CUCA:

104057242(ATHL1)

BRHI:

104489962(ATHL1)

AAM:	106489147(PGGHG)

AROW:

112973763(PGGHG)

NPD:	112948025(PGGHG)

TGT:	104574673(ATHL1)

DNE:	112993815(PGGHG)

SCAM:

104149783(ATHL1)

ASN:	102382785(PGGHG)

AMJ:	102566701(PGGHG)

CPOO:

109318166(PGGHG)

GGN:	109299260(PGGHG)

PSS:	102452333(PGGHG)

CMY:	102947357(PGGHG)

CCAY:

125638888(PGGHG)

DCC:	119857416(PGGHG)

CPIC:

101949278(PGGHG)

TST:	117877389(PGGHG)

CABI:

116822732(PGGHG)

MRV:	120403851(PGGHG)

ACS:	100552045(pgghg)

ASAO:

132771785(PGGHG)

PVT:	110086184(PGGHG)

SUND:

121919827(PGGHG)

PBI:	103064802(PGGHG)

PMUR:

107291105(PGGHG)

CTIG:

120312470(PGGHG)

PGUT:

117665147(PGGHG)

APRI:

131184614(PGGHG)

PTEX:

113440340(PGGHG)

NSS:

113429325

VKO:	123021215(PGGHG)

PMUA:

114600382(PGGHG)

PRAF:

128417472(PGGHG)

ZVI:	118078924 118088834(PGGHG)

HCG:	128340905(PGGHG)

GJA:	107112736(PGGHG)

STOW:

125425776(PGGHG)

EMC:	129323993(PGGHG)

XLA:	108705477(pgghg.L)

XTR:	100496278(pgghg)

NPR:	108784117(PGGHG)

RTEM:

120917544(PGGHG)

BBUF:

120980623(PGGHG)

BGAR:

122920195(PGGHG)

MUO:	115468014(PGGHG)

GSH:	117352397(PGGHG)

DRE:	777617(pgghg)

SRX:	107726850(athl1) 107754371

SANH:

107674819

SGH:

107551409

CCAR:

109071510(pgghg)

CAUA:

113043029

CGIB:

128014171

PTET:

122330635(pgghg)

LROH:

127156346(pgghg)

OMC:	131534788 131534789

PPRM:

120473357

RKG:	130106462(pgghg)

MAMB:

125246848(pgghg)

CIDE:

127506684

MASI:

127435747

TROS:

130551785(pgghg)

TDW:	130413963(pgghg)

MANU:

129434293(pgghg)

IPU:

108274703

IFU:	128618039(pgghg)

PHYP:

113529799(pgghg)

SMEO:

124392887(pgghg)

TFD:	113653967(pgghg)

TVC:	132857147(pgghg)

AMEX:

103031131(pgghg)

CMAO:

118811908(pgghg)

EEE:	113584482(pgghg)

CHAR:

105912032(pgghg)

TRU:	101062094(pgghg)

TFS:	130535691(pgghg)

TNG:	GSTEN00029865G001

LCO:	104928845(pgghg)

NCC:	104956195(athl1)

TBEN:

117491939(pgghg)

CGOB:

115009887(pgghg)

PGEO:

117448416(pgghg)

GACU:

117538852(pgghg)

EMAC:

134873976(pgghg)

ELY:	117261814(pgghg)

EFO:	125887913(pgghg)

PLEP:

121950353(pgghg)

SLUC:

116038726(pgghg)

ECRA:

117949357(pgghg)

ESP:	116676528 116694469

PFLV:

114560021(pgghg)

GAT:	120808687(pgghg)

PPUG:

119196680(pgghg)

AFB:	129108550(pgghg)

CLUM:

117732864(pgghg)

PSWI:

130195837(pgghg)

MSAM:

119893919(pgghg)

SCHU:

122875421(pgghg)

CUD:	121515754(pgghg)

ALAT:

119024343(pgghg)

MZE:	101468880(pgghg)

ONL:	100691439(pgghg)

OAU:	116326290(pgghg)

OLA:	101155845(pgghg)

OML:	112152859(pgghg)

CSAI:

133444114(pgghg)

XMA:	102229355(pgghg)

XCO:	114136298(pgghg)

XHE:	116711420(pgghg)

PRET:

103466501(pgghg)

PFOR:

103141313(athl1)

PLAI:

106964669(athl1)

PMEI:

106933109(athl1)

GAF:	122835741(pgghg)

PPRL:

129367656(pgghg)

CVG:	107100632(athl1)

CTUL:

119787430(pgghg)

GMU:	124877816(pgghg)

NFU:	107388675(athl1)

KMR:	108229896(pgghg)

ALIM:

106521182(athl1)

NWH:	119415788(pgghg)

AOCE:

111577282(pgghg)

MCEP:

125014680(pgghg)

CSEM:

103380281(athl1)

POV:	109623963(pgghg)

SSEN:

122776450(pgghg)

HHIP:

117762923(pgghg)

HSP:	118110268(pgghg)

SMAU:

118315251(pgghg)

LCF:	108886949(pgghg) 108891288

SDU:	111229684(pgghg)

SLAL:

111667069(pgghg)

XGL:	120793538(pgghg)

HCQ:	109507601(pgghg)

SSCV:

125970104

SBIA:

133502618(pgghg)

PEE:	133402384(pgghg)

PTAO:

133478506(pgghg)

BPEC:

110169653(pgghg)

MALB:

109972776(pgghg)

BSPL:

114857078(pgghg)

SJO:	128358767(pgghg)

SASA:

106561823(pgghg)

STRU:

115197720(pgghg)

OTW:	112243931(pgghg)

OMY:	110501276(pgghg)

OGO:

124031677

OKI:	109882080(pgghg)

OKE:	118377207(pgghg)

SALP:

112071753(pgghg)

SNH:	120066505(pgghg)

CCLU:

121531582(pgghg)

ELS:	105029689(pgghg)

SFM:	108940848(pgghg)

PKI:	111848029(pgghg)

AANG:

118215147(pgghg)

PSPA:

121294971(pgghg)

ARUT:

117432325(pgghg)

PSEX:

120535647(pgghg)

LCM:	102363080(PGGHG)

CMK:	103174850(pgghg)

RTP:	109928234(pgghg)

CPLA:

122557883(pgghg)

HOC:	132824180(pgghg)

LERI:

129705784(pgghg)

PMRN:

116945484(PGGHG)

LRJ:	133351299(PGGHG)

BFO:	118422669 118427572

BBEL:

109464648 109485036

CIN:

100183627

SCLV:

120347404

SPU:

762741

LPIC:

129258620 129264601

APLC:

110983330

SKO:	100367252 100368173 100368199 100375069 102801557 102807261

DME:	Dmel_CG16965(CG16965)

DER:

6541527

DSE:

6617683

DSI:	Dsimw501_GD23801(Dsim_GD23801)

DYA:	Dyak_GE18556

DSR:

110183998

DPO:

4817014

DPE:

6588680

DMN:

108162922

DWI:

6642582

DGR:

6566703

DMO:	Dmoj_GI24803

DAZ:

108611620

DNV:

108649416

DHE:

111603770

CCAT:

101461034

BOD:

106622467

BDR:

105229207

RZE:

108366250

AOQ:

129247396

TDA:

119687940

MDE:	101889101 131805476

SCAC:

106081277

LCQ:

111683711

LSQ:

119602617

GFS:

119633381

ECOE:

129949894

CLON:

129608430

AGA:	133392906 4578326

ACOZ:

120957616 120958356 120959147

AARA:

120900865 120901729 120901730

AMER:

121597027 121597088 121597441

ASTE:

118512519 118512520 118512521

AFUN:

125765552 125765553 125765555

AMOU:

128302540 128304671 128304691

AALI:

118461474

AAG:	5564618 5564619 5567690 5576505

AALB:

109417656 109417660 109417661 109417662 109622152 109622154 115255403 115255515

CQU:	CpipJ_CPIJ008851 CpipJ_CPIJ008852 CpipJ_CPIJ008853 CpipJ_CPIJ008854 CpipJ_CPIJ008855

CPII:

120424350 120424354 120424366 120424367 120424368

CNS:

116348022

BCOO:

119076367 119078597

BMOR:

101739191

BMAN:

114246045

MSEX:

115448110

DPL:	KGM_201303

BANY:

112045495

MJU:

123865963

NIQ:

126781408

VCD:

124531443

MCIX:

123669234

PMAC:

106718814

PPOT:

106101409

PXU:

106118146

PRAP:

110993009

PBX:

123707481

PNAP:

125056025

ZCE:

119828533

CCRC:

123706310

LSIN:

126969212

AAGE:

121736765

HAW:

110378758

HZE:

124646374

TNL:

113493163

SLIU:

111352767

OFU:

114356286

PXY:	105386635 105394851

PGW:

126380073

FCD:

110848403

DPX:	DAPPUDRAFT_55687

DMK:

116932146

DPZ:

124349473

EAF:

111706613

TCF:	131890697 131891143

PMEO:

129589013

HRO:	HELRODRAFT_161910 HELRODRAFT_193290 HELRODRAFT_76967

LGI:	LOTGIDRAFT_141357 LOTGIDRAFT_226064

PVUL:

126821012 126821019 126821530 126821531 126821532 126821643

PCAN:

112570055

BGT:	106054488 106072139

GAE:	121371587 121372683

HRF:	124122908 124124759

HRJ:	124254530 124291996

CRG:	105324264 105328484 105340679

CVN:	111129258 111131461 111131462

CANU:

128175239 128176475 128177005

OED:	125650079 125651491 125664030

MYI:	110440546 110440547 110440583

PMAX:

117322791 117324382 117324383

MMER:

123539149

RPHI:

132726411 132738791

DPOL:

127870704

OBI:

106873889

OSN:

115216968

LAK:

106179554

OVI:	T265_13014

NVE:	5507997 5508007 5510775 5511304

EPA:	110233900 110233920 110233925 110253303

ATEN:

116290584 116294426 116294435 116294905

ADF:	107342244 107356873 107356887

AMIL:

114956600 114956601 114965960

PDAM:

113668974 113668976 113674506

SPIS:

111321839 111346317 111346319

DGT:

114523848

XEN:

124434693

HMG:

100207176

HSY:

130621918

MBR:	MONBRDRAFT_16873 MONBRDRAFT_22300

SRE:	PTSG_06584

DDI:	DDB_G0287109(athl1)

DPP:	DICPUDRAFT_97948

DFA:	DFA_10677(athl1)

ACAN:

ACA1_054500 ACA1_368900

NGR:	NAEGRDRAFT_69774(AM32)

NPY:	NPRO_19770

» show all

Reference

1 [PMID:385589]

Authors

Hamazaki H, Hotta K.

Title

Purification and characterization of an alpha-glucosidase specific for hydroxylysine-linked disaccharide of collagen.

Journal

J Biol Chem 254:9682-7 (1979)

Reference

2 [PMID:7460918]

Authors

Hamazaki H, Hotta K.

Title

Enzymatic hydrolysis of disaccharide unit of collagen. Isolation of 2-O-alpha-D-glucopyranosyl-O-beta-D-galactopyranosyl-hydroxylysine glucohydrolase from rat spleens.

Journal

Eur J Biochem 111:587-91 (1980)
DOI:10.1111/j.1432-1033.1980.tb04975.x

Reference

3 [PMID:385599]

Authors

Sternberg M, Spiro RG.

Title

Studies on the catabolism of the hydroxylysine-linked disaccharide units of basement membranes and collagens. Isolation and characterization of a rat kidney alpha-glucosidase of high specificity.

Journal

J Biol Chem 254:10329-36 (1979)

Reference

4 [PMID:26682924]

Authors

Hamazaki H, Hamazaki MH

Title

Catalytic site of human protein-glucosylgalactosylhydroxylysine glucosidase: Three crucial carboxyl residues were determined by cloning and site-directed mutagenesis.

Journal

Biochem Biophys Res Commun 469:357-62 (2016)
DOI:10.1016/j.bbrc.2015.12.005

Sequence

[gga:422991]

Other DBs

ExplorEnz - The Enzyme Database:

3.2.1.107

IUBMB Enzyme Nomenclature:

3.2.1.107

ExPASy - ENZYME nomenclature database:

3.2.1.107

BRENDA, the Enzyme Database:

3.2.1.107

CAS:	72829-45-9

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