KEGG   ENZYME: 3.2.1.171
Entry
EC 3.2.1.171                Enzyme                                 
Name
rhamnogalacturonan hydrolase;
rhamnogalacturonase A;
RGase A;
RG-hydrolase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
Sysname
rhamnogalacturonan alpha-D-GalA-(1->2)-alpha-L-Rha hydrolase
Reaction(IUBMB)
Endohydrolysis of alpha-D-GalA-(1->2)-alpha-L-Rha glycosidic bond in the rhamnogalacturonan I backbone with initial inversion of anomeric configuration releasing oligosaccharides with beta-D-GalA at the reducing end.
Comment
The enzyme is part of the degradation system for rhamnogalacturonan I in Aspergillus aculeatus.
History
EC 3.2.1.171 created 2011
Orthology
K18580  rhamnogalacturonan hydrolase
Genes
TTT: THITE_156575 THITE_2114620 THITE_76240
TMN: UCRPA7_3596
SSCK: SPSK_05216
FGR: FGSG_04689
FVN: FVRRES_00128
FVR: FVEG_03362 FVEG_05697
FOX: FOXG_02509 FOXG_04765
NHE: NECHADRAFT_44944 NECHADRAFT_86762
FFC: NCS54_00947800 NCS54_01342500
FKR: NCS57_00968400 NCS57_01322200
FMU: J7337_002077 J7337_006286
CFJ: CFIO01_07103 CFIO01_10450
CLUP: CLUP02_09920 CLUP02_15944
CHIG: CH63R_12309
PFY: PFICI_03152 PFICI_10114 PFICI_11675
SSL: SS1G_02399 SS1G_06235 SS1G_07039 SS1G_08229
BFU: BCIN_05g04950 BCIN_10g05010 BCIN_12g01100 BCIN_13g02640
PSCO: LY89DRAFT_208229 LY89DRAFT_706183 LY89DRAFT_710038 LY89DRAFT_726790 LY89DRAFT_777221
GLZ: GLAREA_06293
ANI: ANIA_09134
AFM: AFUA_4G00100 AFUA_5G10530
NFI: NFIA_076680
AOR: AO090003000524 AO090005000067 AO090010000484 AO090026000252 AO090038000552 AO090124000009
ANG: An06g02070 An07g01000 An11g06320 An11g08700 An12g00950(rhgA) An14g04200(rhgB)
AFV: AFLA_001166 AFLA_002847 AFLA_006044 AFLA_009037 AFLA_009206
ALUC: AKAW2_12037S AKAW2_12038S AKAW2_41087A AKAW2_52069A AKAW2_80728A AKAW2_81065A
ACHE: ACHE_70599A
APUU: APUU_31609A
PDP: PDIP_19580 PDIP_23710
POU: POX_b02031
TMF: EYB26_006887 EYB26_008602
TRG: TRUGW13939_01757 TRUGW13939_08702 TRUGW13939_08964
PTE: PTT_07842
AALT: CC77DRAFT_730209
ARAB: EKO05_0010001
ZTR: MYCGRDRAFT_77196(MgRHA1)
BCOM: BAUCODRAFT_150330
NPA: UCRNP2_8290 UCRNP2_8853
TML: GSTUM_00004199001
PPL: POSPLDRAFT_45730 POSPLDRAFT_58192
TVS: TRAVEDRAFT_141321 TRAVEDRAFT_62650 TRAVEDRAFT_72935
DSQ: DICSQDRAFT_143747
PCO: PHACADRAFT_133076
SHS: STEHIDRAFT_135809 STEHIDRAFT_141816 STEHIDRAFT_98837
HIR: HETIRDRAFT_42076
PSQ: PUNSTDRAFT_138012
ADL: AURDEDRAFT_199574
FME: FOMMEDRAFT_109953 FOMMEDRAFT_187129 FOMMEDRAFT_86934 FOMMEDRAFT_91227
GTR: GLOTRDRAFT_122631
RSX: RhiXN_09967
CPUT: CONPUDRAFT_162949
SLA: SERLADRAFT_416073
PFP: PFL1_00076 PFL1_04550
PGR: PGTG_15297
PSTR: Pst134EA_013570
MLR: MELLADRAFT_101499 MELLADRAFT_87218 MELLADRAFT_95903
 » show all
Reference
1  [PMID:9115442]
  Authors
Petersen TN, Kauppinen S, Larsen S
  Title
The crystal structure of rhamnogalacturonase A from Aspergillus aculeatus: a right-handed parallel beta helix.
  Journal
Structure 5:533-44 (1997)
DOI:10.1016/S0969-2126(97)00209-8
  Sequence
[ag:AAA64367]
Reference
2  [PMID:7961884]
  Authors
Kofod LV, Kauppinen S, Christgau S, Andersen LN, Heldt-Hansen HP, Dorreich K, Dalboge H
  Title
Cloning and characterization of two structurally and functionally divergent rhamnogalacturonases from Aspergillus aculeatus.
  Journal
J Biol Chem 269:29182-9 (1994)
DOI:10.1016/S0021-9258(19)62028-4
  Sequence
[ag:AAA64367]
Reference
3  [PMID:8720076]
  Authors
Azadi P, O'Neill MA, Bergmann C, Darvill AG, Albersheim P
  Title
The backbone of the pectic polysaccharide rhamnogalacturonan I is cleaved by an endohydrolase and an endolyase.
  Journal
Glycobiology 5:783-9 (1995)
DOI:10.1093/glycob/5.8.783
  Sequence
[ag:AAA64367]
Reference
4  [PMID:15299976]
  Authors
Petersen TN, Christgau S, Kofod LV, Kauppinen S, Johnson AH, Larsen S
  Title
Crystallization and preliminary X-ray studies of rhamnogalacturonase A from Aspergillus aculeatus.
  Journal
Acta Crystallogr D Biol Crystallogr 53:105-7 (1997)
DOI:10.1107/S0907444996010785
Reference
5  [PMID:9464254]
  Authors
Pitson SM, Mutter M, van den Broek LA, Voragen AG, Beldman G
  Title
Stereochemical course of hydrolysis catalysed by alpha-L-rhamnosyl and alpha-D-galacturonosyl hydrolases from Aspergillus aculeatus.
  Journal
Biochem Biophys Res Commun 242:552-9 (1998)
DOI:10.1006/bbrc.1997.8009
  Sequence
[ag:AAA64367]
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.171
IUBMB Enzyme Nomenclature: 3.2.1.171
ExPASy - ENZYME nomenclature database: 3.2.1.171
BRENDA, the Enzyme Database: 3.2.1.171

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Gene (119)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (115)   
   RefGene (3)   
Protein sequence (31)   
   UniProt (16)   
   SWISS-PROT (14)   
   RefSeq(pep) (1)   
DNA sequence (2)   
   RefSeq(nuc) (2)   
Protein domain (4)   
   InterPro (4)   
All databases (156)   

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