KEGG   ENZYME: 3.2.1.179
Entry
EC 3.2.1.179                Enzyme                                 
Name
gellan tetrasaccharide unsaturated glucuronosyl hydrolase;
UGL (ambiguous);
unsaturated glucuronyl hydrolase (ambiguous);
gellan tetrasaccharide unsaturated glucuronyl hydrolase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
Sysname
beta-D-4-deoxy-Delta4-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-D-Glcp beta-D-4-deoxy-Delta4-GlcAp hydrolase
Reaction(IUBMB)
beta-D-4-deoxy-Delta4-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-D-Glcp + H2O = 5-dehydro-4-deoxy-D-glucuronate + beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-D-Glcp [RN:R09831]
Reaction(KEGG)
R09831
Substrate
beta-D-4-deoxy-Delta4-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-D-Glcp [CPD:C19965];
H2O [CPD:C00001]
Product
5-dehydro-4-deoxy-D-glucuronate [CPD:C04053];
beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-D-Glcp [CPD:C19966]
Comment
The enzyme releases 4-deoxy-4(5)-unsaturated D-glucuronic acid from oligosaccharides produced by polysaccharide lyases, e.g. the tetrasaccharide beta-D-4-deoxy-Delta4-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-D-Glcp produced by EC 4.2.2.25, gellan lyase. The enzyme can also hydrolyse unsaturated chondroitin and hyaluronate disaccharides (beta-D-4-deoxy-Delta4-GlcAp-(1->3)-D-GalNAc, beta-D-4-deoxy-Delta4-GlcAp-(1->3)-D-GalNAc6S, beta-D-4-deoxy-Delta4-GlcAp2S-(1->3)-D-GalNAc, beta-D-4-deoxy-Delta4-GlcAp-(1->3)-D-GlcNAc), preferring the unsulfated disaccharides to the sulfated disaccharides.
History
EC 3.2.1.179 created 2011, modified 2016
Orthology
K20831  gellan tetrasaccharide unsaturated glucuronyl hydrolase
Reference
1  [PMID:15148314]
  Authors
Itoh T, Akao S, Hashimoto W, Mikami B, Murata K
  Title
Crystal structure of unsaturated glucuronyl hydrolase, responsible for the degradation of glycosaminoglycan, from Bacillus sp. GL1 at 1.8 A resolution.
  Journal
J Biol Chem 279:31804-12 (2004)
DOI:10.1074/jbc.M403288200
  Sequence
[ag:BAA84216]
Reference
2  [PMID:10441389]
  Authors
Hashimoto W, Kobayashi E, Nankai H, Sato N, Miya T, Kawai S, Murata K
  Title
Unsaturated glucuronyl hydrolase of Bacillus sp. GL1: novel enzyme prerequisite for metabolism of unsaturated oligosaccharides produced by polysaccharide lyases.
  Journal
Arch Biochem Biophys 368:367-74 (1999)
DOI:10.1006/abbi.1999.1305
  Sequence
[ag:BAA84216]
Reference
3  [PMID:16630576]
  Authors
Itoh T, Hashimoto W, Mikami B, Murata K
  Title
Substrate recognition by unsaturated glucuronyl hydrolase from Bacillus sp. GL1.
  Journal
Biochem Biophys Res Commun 344:253-62 (2006)
DOI:10.1016/j.bbrc.2006.03.141
  Sequence
[ag:BAA84216]
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.179
IUBMB Enzyme Nomenclature: 3.2.1.179
ExPASy - ENZYME nomenclature database: 3.2.1.179
BRENDA, the Enzyme Database: 3.2.1.179

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Chemical substance (4)   
   KEGG COMPOUND (4)   
Chemical reaction (3)   
   KEGG REACTION (1)   
   KEGG RCLASS (2)   
Gene (2)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (1)   
Protein sequence (347)   
   UniProt (346)   
   SWISS-PROT (1)   
DNA sequence (550)   
   GenBank (275)   
   EMBL (275)   
Protein domain (4)   
   InterPro (4)   
All databases (910)   

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