KEGG   ENZYME: 3.2.1.204
Entry
EC 3.2.1.204                Enzyme                                 
Name
1,3-alpha-isomaltosidase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
Sysname
1,3-alpha-isomaltohydrolase (configuration-retaining)
Reaction(IUBMB)
cyclobis-(1->6)-alpha-nigerosyl + 2 H2O = 2 isomaltose (overall reaction) [RN:R11788];
(1a) cyclobis-(1->6)-alpha-nigerosyl + H2O = alpha-isomaltosyl-(1->3)-isomaltose [RN:R11789];
(1b) alpha-isomaltosyl-(1->3)-isomaltose + H2O = 2 isomaltose [RN:R11790]
Reaction(KEGG)
R11788 R11789 R11790
Substrate
cyclobis-(1->6)-alpha-nigerosyl;
H2O [CPD:C00001];
alpha-isomaltosyl-(1->3)-isomaltose
Product
isomaltose [CPD:C00252];
alpha-isomaltosyl-(1->3)-isomaltose
Comment
The enzyme, characterized from the bacteria Bacillus sp. NRRL B-21195 and Kribbella flavida, participates in the degradation of starch. The cyclic tetrasaccharide cyclobis-(1->6)-alpha-nigerosyl is formed from starch extracellularly and imported into the cell, where it is degraded to glucose.
History
EC 3.2.1.204 created 2017
Orthology
K22307  1,3-alpha-isomaltosidase
Genes
MBIN: LXM64_10370
MHUM: NNL39_09760
NAEI: GCM126_24290
CELC: K5O09_03055
CCHE: NP064_12685
HALT: IM660_06180
RHAL: LQF10_13525
DEM: LGT36_006970
NCA: Noca_4630
KFL: Kfla_1895
KSL: OG809_21350
NOA: BKM31_21905
NGN: LCN96_29630
NAKA: H7F38_01825 H7F38_19770
DVC: Dvina_30560
TPY: CQ11_05330
TPYO: X956_02375
AUO: R3I39_10895
AYM: YM304_31640
 » show all
Reference
1  [PMID:15063208]
  Authors
Kim YK, Kitaoka M, Hayashi K, Kim CH, Cote GL
  Title
Purification and characterization of an intracellular cycloalternan-degrading enzyme from Bacillus sp. NRRL B-21195.
  Journal
Carbohydr Res 339:1179-84 (2004)
DOI:10.1016/j.carres.2004.02.008
Reference
2  [PMID:27302067]
  Authors
Tagami T, Miyano E, Sadahiro J, Okuyama M, Iwasaki T, Kimura A
  Title
Two Novel Glycoside Hydrolases Responsible for the Catabolism of Cyclobis-(1-->6)-alpha-nigerosyl.
  Journal
J Biol Chem 291:16438-47 (2016)
DOI:10.1074/jbc.M116.727305
  Sequence
[kfl:Kfla_1895]
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.204
IUBMB Enzyme Nomenclature: 3.2.1.204
ExPASy - ENZYME nomenclature database: 3.2.1.204
BRENDA, the Enzyme Database: 3.2.1.204

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Chemical substance (4)   
   KEGG COMPOUND (4)   
Chemical reaction (5)   
   KEGG REACTION (3)   
   KEGG RCLASS (2)   
Gene (68)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (20)   
   KEGG MGENES (47)   
Protein sequence (2)   
   UniProt (1)   
   SWISS-PROT (1)   
Protein domain (9)   
   InterPro (9)   
All databases (88)   

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