KEGG ENZYME: 3.2.1.212

ENZYME: 3.2.1.212

Entry

EC 3.2.1.212                Enzyme

Name

endo-(1->4)-fucoidanase;
alpha-L-fucosidase (incorrect);
poly(1,4-alpha-L-fucoside-2/3-sulfate) glycanohydrolase

Class

Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

Sysname

poly[(1->4)-alpha-L-fucoside-2/3-sulfate] glycanohydrolase

Reaction(IUBMB)

endohydrolysis of (1->4)-alpha-L-fucoside linkages in fucan

Comment

The enzyme specifically hydrolyses (1->4)-alpha-L-fucoside linkages in fucan. Fucans are found mainly in different species of seaweed and are sulfated polysaccharides with a backbone of (1->3)-linked or alternating (1->3)- and (1->4)-linked alpha-L-fucopyranosyl residues. In the literature, the sulfated polysaccharides are often called fucoidans. Fucoidans include polysaccharides with a relatively low proportion of fucose and some polysaccharides that have a backbone composed of other saccharides with fucose in the branching side chains. The sulfation of the alpha-L-fucopyranosyl residues may occur at positions 2 and 3. The enzyme degrades fucan to sulfated alpha-L-fucooligosaccharides but neither L-fucose nor small fucooligosaccharides are produced.

History

EC 3.2.1.212 created 1972 as EC 3.2.1.44, part transferred 2020 to EC 3.2.1.212

Reference

Authors

Thanassi, N.M. and Nakada, H.I.

Title

Enzymic degradation of fucoidan by enzymes from the hepatopancreas of abalone, Halotus species.

Journal

Arch Biochem Biophys 118:172-177 (1967)

Reference

2 [PMID:12626402]

Authors

Berteau O, Mulloy B

Title

Sulfated fucans, fresh perspectives: structures, functions, and biological properties of sulfated fucans and an overview of enzymes active toward this class of polysaccharide.

Journal

Glycobiology 13:29R-40R (2003)
DOI:10.1093/glycob/cwg058

Reference

3 [PMID:16222488]

Authors

Descamps V, Colin S, Lahaye M, Jam M, Richard C, Potin P, Barbeyron T, Yvin JC, Kloareg B

Title

Isolation and culture of a marine bacterium degrading the sulfated fucans from marine brown algae.

Journal

Mar Biotechnol (NY) 8:27-39 (2006)
DOI:10.1007/s10126-005-5107-0

Reference

4 [PMID:18467852]

Authors

Kim WJ, Kim SM, Lee YH, Kim HG, Kim HK, Moon SH, Suh HH, Jang KH, Park YI

Title

Isolation and characterization of marine bacterial strain degrading fucoidan from Korean Undaria pinnatifida Sporophylls.

Journal

J Microbiol Biotechnol 18:616-23 (2008)

Reference

5 [PMID:23852092]

Authors

Silchenko AS, Kusaykin MI, Kurilenko VV, Zakharenko AM, Isakov VV, Zaporozhets TS, Gazha AK, Zvyagintseva TN

Title

Hydrolysis of fucoidan by fucoidanase isolated from the marine bacterium, Formosa algae.

Journal

Mar Drugs 11:2413-30 (2013)
DOI:10.3390/md11072413

Reference

Authors

Silchenko, A.S., Kusaykin, M.I., Zakharenko, A.M., Menshova, R.V., Khanh, H.H.N., Dmitrenok, P.S., Isakov, V.V., Zvyagintseva, T.N.

Title

Endo-1,4-fucoidanase from vietnamese marine mollusk Lambis sp. which producing sulphated fucooligosaccharides.

Journal

J Mol Catal B 102:154-160 (2014)

Reference

7 [PMID:28031251]

Authors

Silchenko AS, Ustyuzhanina NE, Kusaykin MI, Krylov VB, Shashkov AS, Dmitrenok AS, Usoltseva RV, Zueva AO, Nifantiev NE, Zvyagintseva TN

Title

Expression and biochemical characterization and substrate specificity of the fucoidanase from Formosa algae.

Journal

Glycobiology 27:254-263 (2017)
DOI:10.1093/glycob/cww138

Other DBs

ExplorEnz - The Enzyme Database:

3.2.1.212

IUBMB Enzyme Nomenclature:

3.2.1.212

ExPASy - ENZYME nomenclature database:

3.2.1.212

BRENDA, the Enzyme Database:

3.2.1.212

All links

Protein sequence (1)   
   PDBSTR (1)   
DNA sequence (12)   
   GenBank (6)   
   EMBL (6)   
3D Structure (1)   
   PDB (1)   
All databases (14)   

Download RDF

DBGET integrated database retrieval system