KEGG   ENZYME: 3.2.1.215
Entry
EC 3.2.1.215                Enzyme                                 
Name
arabinogalactan exo alpha-(1,3)-alpha-D-galactosyl-(1->3)-L-arabinofuranosidase (non-reducing end);
3-O-alpha-D-galactosyl-alpha-L-arabinofuranosidase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
Sysname
type II arabinogalactan exo alpha-(1,3)-[alpha-D-galactosyl-(1->3)-L-arabinofuranose] hydrolase (non-reducing end)
Reaction(IUBMB)
Hydrolysis of alpha-D-Galp-(1->3)-L-Araf disaccharides from non-reducing terminals in branches of type II arabinogalactan attached to proteins.
Comment
The enzyme, characterized from the bacterium Bifidobacterium longum, specifically hydrolyses alpha-D-Galp-(1->3)-L-Araf disaccharides from the non-reducing terminal of arabinogalactan using an exo mode of action. It is particularly active with gum arabic arabinogalactan, a type II arabinogalactan produced by acacia trees. The enzyme can also hydrolyse beta-L-Arap-(1->3)-L-Araf disaccharides, but this activity is significantly lower.
History
EC 3.2.1.215 created 2021
Orthology
K25603  3-O-alpha-D-galactosyl-alpha-L-arabinofuranosidase
Reference
1  [PMID:33674431]
  Authors
Sasaki Y, Horigome A, Odamaki T, Xiao JZ, Ishiwata A, Ito Y, Kitahara K, Fujita K.
  Title
Novel 3-O-alpha-d-Galactosyl-alpha-l-Arabinofuranosidase for the Assimilation of Gum Arabic Arabinogalactan Protein in Bifidobacterium longum subsp. longum.
  Journal
Appl Environ Microbiol 87:e02690-20 (2021)
DOI:10.1128/AEM.02690-20
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.215
IUBMB Enzyme Nomenclature: 3.2.1.215
ExPASy - ENZYME nomenclature database: 3.2.1.215
BRENDA, the Enzyme Database: 3.2.1.215

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