Entry
Name
exo-acting protein-alpha-N-acetylgalactosaminidase;
Nag31
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
[protein]-N-acetyl-alpha-D-galactosalaminyl-(L-serine/L-threonine) N-acetylgalactosaminohydrolase
Reaction(IUBMB)
a [protein]-N-acetyl-alpha-D-galactosalaminyl-(L-serine/L-threonine) + H2O = a [protein]-(L-serine/L-threonine) + N-acetyl-D-galactosamine
Substrate
[protein]-N-acetyl-alpha-D-galactosalaminyl-(L-serine/L-threonine);
H2O [CPD:
C00001 ]
Product
[protein]-(L-serine/L-threonine);
N-acetyl-D-galactosamine [CPD:
C01132 ]
Comment
The enzyme, which belongs to the glycosylhydrolase 31 (GH31) family, is an exo-acting alpha-N-acetylgalactosaminidase that acts on the innermost alpha-GalNAc residues at the core of O-glycans when no other saccharides are attached to it. Unlike EC
3.2.1.49 , alpha-N-acetylgalactosaminidase, it is not able to act on blood group A antigen.
History
EC 3.2.1.217 created 2022
Orthology
K25767 exo-acting protein-alpha-N-acetylgalactosaminidase
Genes
SSTE : SAMEA4384403_1530(yicI)
BTHO : Btheta7330_05128(yicI_6)
BCAC : CGC64_02900 CGC64_03580
BFC : BacF7301_03620 BacF7301_04830
SMIZ : 4412673_02693(yicI_2)
STHA : NCTC11429_00510(yicI_2)
» show all
Taxonomy
Reference
Authors
Rahfeld P, Wardman JF, Mehr K, Huff D, Morgan-Lang C, Chen HM, Hallam SJ, Withers SG.
Title
Prospecting for microbial alpha-N-acetylgalactosaminidases yields a new class of GH31 O-glycanase.
Journal
Sequence
Reference
Authors
Miyazaki T, Park EY.
Title
Crystal structure of the Enterococcus faecalis alpha-N-acetylgalactosaminidase, a member of the glycoside hydrolase family 31.
Journal
Sequence
Reference
Authors
Ikegaya M, Miyazaki T, Park EY.
Title
Biochemical characterization of Bombyx mori alpha-N-acetylgalactosaminidase belonging to the glycoside hydrolase family 31.
Journal
Reference
Authors
Miyazaki T, Ikegaya M, Alonso-Gil S.
Title
Structural and mechanistic insights into the substrate specificity and hydrolysis of GH31 alpha-N-acetylgalactosaminidase.
Journal
Sequence
Other DBs
ExPASy - ENZYME nomenclature database: 3.2.1.217