KEGG ENZYME: 3.2.1.223

ENZYME: 3.2.1.223

Entry

EC 3.2.1.223                Enzyme

Name

arabinogalactan exo alpha-(1,3)-beta-L-arabinopyranosyl-(1->3)-L-arabinofuranosidase (non-reducing end);
3-O-beta-L-arabinopyranosyl-alpha-L-arabinofuranosidase;
AAfase

Class

Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

Sysname

type II arabinogalactan exo alpha-(1,3)-[beta-L-arabinopyranosyl-(1->3)-L-arabinofuranose] hydrolase (non-reducing end)

Reaction(IUBMB)

Hydrolysis of beta-L-Arap-(1->3)-L-Araf disaccharides from non-reducing terminals in branches of type II arabinogalactan attached to proteins.

Comment

The enzyme, characterized from the bacterium Bifidobacterium pseudocatenulatum, specifically hydrolyses beta;-L-Arap-(1->3)-L-Araf disaccharides from the non-reducing terminal of arabinogalactan using an exo mode of action. It is active with arabinogalactan-proteins (AGPs) containing type II arabinogalactans such as gum arabic AGP and larch AGP. The enzyme can also hydrolyse alpha-D-Galp-(1->3)-L-Araf disaccharides (cf. EC 3.2.1.215) with a much lower activity.

History

EC 3.2.1.223 created 2023

Reference

Authors

Sasaki, Y., Yanagita, M., Hashiguchi, M., Horigome, A., Xiao, J. Z., Odamaki, T., Kitahara, K. and Fujita, K.

Title

Assimilation of arabinogalactan side chains with novel 3-O-beta-L-arabinopyranosyl-alpha-L-arabinofuranosidase in Bifidobacterium pseudocatenulatum.

Journal

Microbiome Res Rep 2:#12 (2023)

Other DBs

ExplorEnz - The Enzyme Database:

3.2.1.223