KEGG ENZYME: 3.2.1.81

ENZYME: 3.2.1.81

Entry

EC 3.2.1.81                 Enzyme

Name

beta-agarase;
agarase (ambiguous);
AgaA;
AgaB;
endo-beta-agarase;
agarose 3-glycanohydrolase (incorrect)

Class

Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

Sysname

agarose 4-glycanohydrolase

Reaction(IUBMB)

Hydrolysis of (1->4)-beta-D-galactosidic linkages in agarose, giving the tetramer as the predominant product

Comment

Also acts on porphyran, but more slowly [1]. This enzyme cleaves the beta-(1->4) linkages of agarose in a random manner with retention of the anomeric-bond configuration, producing beta-anomers that give rise progressively to alpha-anomers when mutarotation takes place [6]. The end products of hydrolysis are neoagarotetraose and neoagarohexaose in the case of AgaA from the marine bacterium Zobellia galactanivorans, and neoagarotetraose and neoagarobiose in the case of AgaB [6].

History

EC 3.2.1.81 created 1972, modified 2006

Orthology

K01219

beta-agarase

Genes

VAS:	GT360_06905

SJA:	SJ2017_2075

PBW:	D172_008050

PAGA:

PAGA_a1786

ASP:	AOR13_1359

ASQ:	AVL57_11855

AAW:	AVL56_11045

ALE:	AV939_10915

ALZ:	AV940_10685

GAG:	Glaag_1821 Glaag_2339

CATE:

C2869_02075 C2869_07230

CEK:	D0B88_11585 D0B88_11590

CEG:	D0C16_02330 D0C16_02335 D0C16_02385

SDE:	Sde_1175(aga16B)

SAGA:

M5M_16890

MTHD:

A3224_10290 A3224_13515

MAGA:

Mag101_05925

SCO:	SCO3471(dagA)

CAA:	Caka_0054 Caka_0364 Caka_0553

VBS:	EGM51_00030

CCOP:

Mal65_10000(agaB)

PHM:	PSMK_13810 PSMK_31770

BUN:	Bun01g_38580(dagA_1)

EST:	DN752_04920

FLM:	MY04_5423 MY04_5483

FLL:	EI427_25440

FYA:	KMW28_21570

FKA:	KM029_21095

RCG:	N7E81_10840 N7E81_17460

FFA:	FFWV33_00015 FFWV33_00795

MARM:

YQ22_01755

CAO:	Celal_2463 Celal_3665 Celal_3979

CLY:	Celly_0445 Celly_2860

CLH:	IX49_01925

CBAL:

M667_09675 M667_09860

CBAT:

M666_09600 M666_09850

COMN:

PBN93_02100 PBN93_02245 PBN93_14305

ZGA:	ZOBELLIA_3573(agaB) ZOBELLIA_4203(agaA) ZOBELLIA_4243(agaD)

ZLA:	Q5W13_00285

PRN:	BW723_05770 BW723_07545

TJE:	TJEJU_1794(agaQ) TJEJU_2943(agaZ)

WFU:	AXE80_09700

TAJ:	C1A40_08665 C1A40_12485 C1A40_12565

AQA:	D1815_13830

AQD:	D1816_23605

AQE:	NBT05_08985

» show all

Reference

1 [PMID:5386190]

Authors

Duckworth M, Turvey JR.

Title

The action of a bacterial agarase on agarose, porphyran and alkali-treated porphyran.

Journal

Biochem J 113:687-92 (1969)
DOI:10.1042/bj1130687

Reference

2 [PMID:12970344]

Authors

Allouch J, Jam M, Helbert W, Barbeyron T, Kloareg B, Henrissat B, Czjzek M.

Title

The three-dimensional structures of two beta-agarases.

Journal

J Biol Chem 278:47171-80 (2003)
DOI:10.1074/jbc.M308313200

Sequence

[zga:ZOBELLIA_4203 ZOBELLIA_3573]

Reference

3 [PMID:15170112]

Authors

Ohta Y, Nogi Y, Miyazaki M, Li Z, Hatada Y, Ito S, Horikoshi K.

Title

Enzymatic properties and nucleotide and amino acid sequences of a thermostable beta-agarase from the novel marine isolate, JAMB-A94.

Journal

Biosci Biotechnol Biochem 68:1073-81 (2004)
DOI:10.1271/bbb.68.1073

Reference

4 [PMID:15088129]

Authors

Ohta Y, Hatada Y, Nogi Y, Miyazaki M, Li Z, Akita M, Hidaka Y, Goda S, Ito S, Horikoshi K.

Title

Enzymatic properties and nucleotide and amino acid sequences of a thermostable beta-agarase from a novel species of deep-sea Microbulbifer.

Journal

Appl Microbiol Biotechnol 64:505-14 (2004)
DOI:10.1007/s00253-004-1573-y

Reference

5 [PMID:8517750]

Authors

Sugano Y, Terada I, Arita M, Noma M, Matsumoto T.

Title

Purification and characterization of a new agarase from a marine bacterium, Vibrio sp. strain JT0107.

Journal

Appl Environ Microbiol 59:1549-54 (1993)
DOI:10.1128/AEM.59.5.1549-1554.1993

Reference

6 [PMID:15456406]

Authors

Jam M, Flament D, Allouch J, Potin P, Thion L, Kloareg B, Czjzek M, Helbert W, Michel G, Barbeyron T

Title

The endo-beta-agarases AgaA and AgaB from the marine bacterium Zobellia galactanivorans: two paralogue enzymes with different molecular organizations and catalytic behaviours.

Journal

Biochem J 385:703-13 (2005)
DOI:10.1042/BJ20041044

Sequence

[zga:ZOBELLIA_4203 ZOBELLIA_3573]

Other DBs

ExplorEnz - The Enzyme Database:

3.2.1.81

IUBMB Enzyme Nomenclature:

3.2.1.81

ExPASy - ENZYME nomenclature database:

3.2.1.81

BRENDA, the Enzyme Database:

3.2.1.81

CAS:	37288-57-6

DBGET integrated database retrieval system