KEGG ENZYME: 3.2.1.82

ENZYME: 3.2.1.82

Entry

EC 3.2.1.82                 Enzyme

Name

exo-poly-alpha-digalacturonosidase;
pehX (gene name);
poly(1,4-alpha-D-galactosiduronate) digalacturonohydrolase;
exopolygalacturonosidase (misleading);
poly[(1->4)-alpha-D-galactosiduronate] digalacturonohydrolase;
exo-poly-alpha-galacturonosidase

Class

Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

Sysname

poly[(1->4)-alpha-D-galactosiduronate] non-reducing-end-digalacturonohydrolase

Reaction(IUBMB)

[(1->4)-alpha-D-galacturonosyl]n + H2O = alpha-D-galacturonosyl-(1->4)-D-galacturonate + [(1->4)-alpha-D-galacturonosyl]n-2 [RN:R02360 R06239]

Reaction(KEGG)

R02360 R06239(G)

Substrate

[(1->4)-alpha-D-galacturonosyl]n [CPD:C00470];
H2O [CPD:C00001]

Product

alpha-D-galacturonosyl-(1->4)-D-galacturonate [CPD:C02273];
[(1->4)-alpha-D-galacturonosyl]n-2

Comment

The enzyme, characterized from bacteria, hydrolyses the second alpha-1,4-glycosidic bond from the non-reducing end of polygalacturonate, releasing digalacturonate.

History

EC 3.2.1.82 created 1972, modified 2019

Orthology

K18650

exo-poly-alpha-galacturonosidase

Genes

KOX:

KOX_13235

KOE:

A225_1464

KOY:	J415_24315

KOM:	HR38_11985

KOK:	KONIH1_07185

KOC:	AB185_28860

KLL:	BJF97_07630

KGR:	JJJ10_20875

KPAS:

LUW96_14020

KLC:	K7H21_20890

KLM:	BWI76_07155

KIE:	NCTC12125_00461(pehX_1)

KAS:	KATP_14600(pehX)

KLU:	K7B04_21690

KCY:	RIN60_07560

SYM:	K6K13_14425

YEN:	YE0164(pehX)

YEP:	YE105_C0167

YEY:

Y11_33621

YEL:	LC20_08995

YEW:	CH47_3321(pehX)

YET:	CH48_1856(pehX)

YEF:	FORC2_3934

YEE:	YE5303_41081(pehX)

YSI:	BF17_08810

YIN:	CH53_1967(pehX)

YRO:	CH64_2753(pehX)

YAK:	ACZ76_10035

YMA:	DA391_22030

YHI:	D5F51_00875

YCA:	F0T03_00845

YMO:	HRD69_04365

YAS:	N0H69_21105

YEG:	PL78_07565

SFG:	AV650_16660

SFO:	Z042_11045 Z042_19390

PROD:

PCO85_21680

SERA:

Ser39006_003785

SERQ:

CWC46_03785

ECA:	ECA3111(pehX)

PATR:

EV46_15395

PATO:

GZ59_31190(pehX)

PCT:

PC1_2862

PCC:	PCC21_029350

PCV:	BCS7_14740

PWA:	Pecwa_1410

PPAR:

A8F97_11825

PEC:

W5S_1293

PWS:	A7983_01380

PPOA:

BJK05_09555

PBRA:

B5S52_07160

PARI:

I2D83_07120

PAQU:

DMB82_0007000

PVZ:	OA04_31120(pehX)

PPAV:

LOZ86_14440

PQU:	IG609_006165

PPOO:

LW347_14610

PCAC:

OI450_06250

PACB:

M9782_16940

DDD:	Dda3937_00261(pehX) Dda3937_00262(pehW) Dda3937_00263(pehV)

DZE:	Dd1591_0152 Dd1591_0153

DDC:	Dd586_3904

DZC:	W909_19025

DSO:	A4U42_07660 A4U42_07670 A4U42_07675

CED:	LH89_13825

DFN:	CVE23_21045

DDQ:	DDI_4012 DDI_4013

DAQ:	DAQ1742_04251(pehX)

DIC:	Dpoa569_000250

DLC:	O1Q98_12795

DDA:	Dd703_0200

BGJ:	AWC36_19995

BRB:	EH207_12225

BNG:	EH206_01265

BIZ:	HC231_01250

LBQ:	CKQ53_04220

LPOP:

I6N93_00885

PPF:

Pput_1884

PPB:	PPUBIRD1_1854

PPI:

YSA_08947

PPX:	T1E_2073(pehX)

PPUT:

L483_21750

PFV:	Psefu_3160

PFS:	PFLU_2286(pehX)

PTV:	AA957_24405

PMUD:

NCTC8068_02353(pehX)

PSES:

PSCI_0280

PPSH:

G5J76_17945

PXA:	KSS93_18540

PTZ:	HU718_011220

PASG:

KSS96_12180

PTAE:

NCTC10697_02681(pehX)

PGF:	J0G10_11745

PKM:	PZ739_14175

PTAI:

ICN73_23245

PKK:	QQ992_18090

GSN:	YC6258_03209

RSO:	RSc1756(pehB)

RSE:

F504_1633

RPU:	CDC45_09050

RNC:	GO999_08415

RSC:	RCFBP_11765(pehB)

RSL:	RPSI07_0327(pehB)

RSN:	RSPO_c01791(pehB)

RSM:	CMR15_11765(pehB)

RSY:	RSUY_19490(pehX)

RSG:	JK151_03080

NNV:	QNH39_20875

PMS:	KNP414_02001

PMQ:	PM3016_2299

PMW:

B2K_11610

PBD:	PBOR_08815

PAEA:

R70723_08115

PAEE:

R70331_08155

PKB:	B4V02_22345

NTR:	B0W44_12550

LHB:	D1010_14310

EGA:	AL523_00840

EIE:	ENLAB_09950

CAC:

CA_C3684

CAE:	SMB_G3725(pehX)

CAY:

CEA_G3691

CLJ:	CLJU_c29360

CSR:	Cspa_c52840

CSB:	CLSA_c18950(pehX)

CAH:	CAETHG_0930

CRW:	CROST_001180(pehX_1) CROST_001190(pehX_2) CROST_002390(pehX_3)

CFB:	CLFE_000630(pehX_1) CLFE_001810(pehX_2) CLFE_001820(pehX_3) CLFE_040580

CAUN:

CLAUR_021790 CLAUR_027690(pehX_1) CLAUR_028900(pehX_2) CLAUR_028910(pehX_3)

CGEL:

psyc5s11_19170(pehX)

BPB:	bpr_I2854(pgl28B)

BHU:

bhn_I2679

SRI:	SELR_01400

PUF:

UFO1_0808

PFT:

JBW_03716

LXL:	KDY119_03537(pehX)

ARZ:	AUT26_18105

ARX:	ARZXY2_4273

PUE:	FV140_19810

PNV:	JMY29_18500

PRV:	G7070_02070

ACTQ:

OG417_33020

BLEM:

BL8807_11500

BEU:	BE0216_11810

BNK:	KIM372_05040

FGG:	FSB75_10870

FLN:

FLA_0076

LACS:

H4075_11225

MMAB:

HQ865_23305

SBX:	CA265_05770

» show all

Reference

1 [PMID:5661621]

Authors

Hasegawa S, Nagel CW.

Title

Isolation of an oligogalacturonate hydrolase from a Bacillus specie.

Journal

Arch Biochem Biophys 124:513-20 (1968)
DOI:10.1016/0003-9861(68)90360-3

Reference

Authors

Hatanaka, C. and Ozawa, J.

Title

Enzymic degradation of pectic acid. XIII. New exopolygalacturonase producing digalacturonic acid from pectic acid.

Journal

J Agric Chem Soc Jpn 43:764-772 (1968)

Reference

Authors

Hatanaka, C. and Ozawa, J.

Title

Ber des O'Hara Inst 15:47 (1971)

Reference

4 [PMID:2168372]

Authors

He SY, Collmer A

Title

Molecular cloning, nucleotide sequence, and marker exchange mutagenesis of the exo-poly-alpha-D-galacturonosidase-encoding pehX gene of Erwinia chrysanthemi EC16.

Journal

J Bacteriol 172:4988-95 (1990)
DOI:10.1128/JB.172.9.4988-4995.1990

Sequence

[dze:Dd1591_0152]

Other DBs

ExplorEnz - The Enzyme Database:

3.2.1.82

IUBMB Enzyme Nomenclature:

3.2.1.82

ExPASy - ENZYME nomenclature database:

3.2.1.82

BRENDA, the Enzyme Database:

3.2.1.82

CAS:	37288-58-7

DBGET integrated database retrieval system