KEGG   ENZYME: 3.2.1.83
Entry
EC 3.2.1.83                 Enzyme                                 
Name
kappa-carrageenase;
kappa-carrageenan 4-beta-D-glycanohydrolase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
Sysname
kappa-carrageenan 4-beta-D-glycanohydrolase (configuration-retaining)
Reaction(IUBMB)
Endohydrolysis of (1->4)-beta-D-linkages between D-galactose 4-sulfate and 3,6-anhydro-D-galactose in kappa-carrageenans
Comment
The main products of hydrolysis are neocarrabiose-sulfate and neocarratetraose-sulfate [5]. Unlike EC 3.2.1.157 (iota-carrageenase), but similar to EC 3.2.1.81 (beta-agarase), this enzyme proceeds with retention of the anomeric configuration.
History
EC 3.2.1.83 created 1972, modified 2006
Orthology
K20846  kappa-carrageenase
Genes
VAS: GT360_19020 GT360_19045
SJA: SJ2017_3891
PBW: D172_007780
PCAR: PC2016_3912
SMAM: Mal15_67660(cgkA)
SNEP: Enr13x_43520(cgkA)
CCOP: Mal65_11930(cgkA)
Reference
1  [PMID:5972647]
  Authors
Weigl J, Yaphe W.
  Title
The enzymic hydrolysis of carrageenan by Pseudomonas carrageenovora: purification of a kappa-carrageenase.
  Journal
Can J Microbiol 12:939-47 (1966)
DOI:10.1139/m66-127
Reference
2  [PMID:1915370]
  Authors
Potin P, Sanseau A, Le Gall Y, Rochas C, Kloareg B.
  Title
Purification and characterization of a new kappa-carrageenase from a marine Cytophaga-like bacterium.
  Journal
Eur J Biochem 201:241-7 (1991)
DOI:10.1111/j.1432-1033.1991.tb16280.x
Reference
3  [PMID:7737202]
  Authors
Potin P, Richard C, Barbeyron T, Henrissat B, Gey C, Petillot Y, Forest E, Dideberg O, Rochas C, Kloareg B.
  Title
Processing and hydrolytic mechanism of the cgkA-encoded kappa-carrageenase of Alteromonas carrageenovora.
  Journal
Eur J Biochem 228:971-5 (1995)
DOI:10.1111/j.1432-1033.1995.0971m.x
Reference
4  [PMID:10089334]
  Authors
Michel G, Barbeyron T, Flament D, Vernet T, Kloareg B, Dideberg O.
  Title
Expression, purification, crystallization and preliminary x-ray analysis of the kappa-carrageenase from Pseudoalteromonas carrageenovora.
  Journal
Acta Crystallogr D Biol Crystallogr 55:918-20 (1999)
DOI:10.1107/S0907444998018526
Reference
5  [PMID:11435116]
  Authors
Michel G, Chantalat L, Duee E, Barbeyron T, Henrissat B, Kloareg B, Dideberg O
  Title
The kappa-carrageenase of P. carrageenovora features a tunnel-shaped active site: a novel insight in the evolution of Clan-B glycoside hydrolases.
  Journal
Structure 9:513-25 (2001)
DOI:10.1016/S0969-2126(01)00612-8
  Sequence
[ag:CAA50624]
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.83
IUBMB Enzyme Nomenclature: 3.2.1.83
ExPASy - ENZYME nomenclature database: 3.2.1.83
BRENDA, the Enzyme Database: 3.2.1.83
CAS: 37288-59-8

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Gene (10)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (9)   
Protein sequence (312)   
   UniProt (268)   
   SWISS-PROT (1)   
   RefSeq(pep) (36)   
   PDBSTR (7)   
DNA sequence (144)   
   RefSeq(nuc) (38)   
   GenBank (53)   
   EMBL (53)   
3D Structure (3)   
   PDB (3)   
Protein domain (7)   
   InterPro (6)   
   PROSITE(DOC) (1)   
All databases (476)   

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