KEGG ENZYME: 3.2.2.5

ENZYME: 3.2.2.5

Entry

EC 3.2.2.5                  Enzyme

Name

NAD+ glycohydrolase;
NAD glycohydrolase;
nicotinamide adenine dinucleotide glycohydrolase;
beta-NAD+ glycohydrolase;
DPNase (ambiguous);
NAD hydrolase (ambiguous);
diphosphopyridine nucleosidase (ambiguous);
nicotinamide adenine dinucleotide nucleosidase (ambiguous);
NAD nucleosidase (ambiguous);
DPN hydrolase (ambiguous);
NADase (ambiguous);
nga (gene name);
NAD+ nucleosidase

Class

Hydrolases;
Glycosylases;
Hydrolysing N-glycosyl compounds

Sysname

NAD+ glycohydrolase

Reaction(IUBMB)

NAD+ + H2O = ADP-D-ribose + nicotinamide [RN:R00102]

Reaction(KEGG)

R00102

Substrate

NAD+ [CPD:C00003];
H2O [CPD:C00001]

Product

ADP-D-ribose [CPD:C00301];
nicotinamide [CPD:C00153]

Comment

This enzyme catalyses the hydrolysis of NAD+, without associated ADP-ribosyl cyclase activity (unlike the metazoan enzyme EC 3.2.2.6, bifunctional ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase). The enzyme from Group A streptococci has been implicated in the pathogenesis of diseases such as streptococcal toxic shock-like syndrome (STSS) and necrotizing fasciitis. The enzyme from the venom of the snake Agkistrodon acutus also catalyses EC 3.6.1.5, apyrase [3].

History

EC 3.2.2.5 created 1961, modified 2013

Pathway

ec00760

Nicotinate and nicotinamide metabolism

ec01100

Metabolic pathways

Orthology

K18153

NAD+ glycohydrolase

K19980

ADP-ribosyltransferase 2 / NAD+ glycohydrolase

Genes

MMU:	11871(Art2a) 11872(Art2b)

MCAL:

110298813

MPAH:

110336159

RNO:	293152(Art2b)

MCOC:

116100665 116101301

ANU:	117693579 117695831

MUN:

110550800

CGE:	100750708 100751294 100771938 100772218 100772803

MAUA:

101824175 101839372 121134115 121144298

PROB:

127227991 127227992

PLEU:

114698648 114698743 114698744 114706661 119086245

MFOT:

126488848

NGI:	103748863 103749849

HGL:

106007650

CPOC:

100714269 100717061

CCAN:

109678990

NCAR:

124958806

MMMA:

107135550

OPI:

101536559

TUP:

102487179

GVR:

103607714

BACU:

103000152

BMUS:

118899688

MYB:

102252898

MYD:

102773420

MMYO:

118664685 118664687

MLF:	102425244 102438022 102438936

EFUS:

103293570

MNA:

107533657

DRO:

112315853

SHON:

119002660

AJM:

119042110

PDIC:

114498648 114500760

PHAS:

123805524 123816978

MMF:

118627508

PPAM:

129072723

RFQ:

117031024

PALE:

102895427

PGIG:

120590522

PVP:

105303689

SARA:

101541382

ETF:

123522055

DNM:

111761064

CPLA:

122545933

FCD:

110841647

SPY:	SPy_0165(nga)

SPZ:	M5005_Spy0139(nga)

SPYM:

M1GAS476_0178(nga)

SPYA:

A20_0190(nga)

SPM:	spyM18_0163(nga)

SPG:	SpyM3_0128(nga)

SPS:

SPs0130

SPH:	MGAS10270_Spy0141(nga)

SPI:	MGAS10750_Spy0145(nga)

SPJ:	MGAS2096_Spy0146(nga)

SPK:	MGAS9429_Spy0141(nga)

SPF:	SpyM50134(nga)

SPA:	M6_Spy0186

SPB:	M28_Spy0137(nga)

STG:	MGAS15252_0175(nga)

STX:	MGAS1882_0175(nga)

SOZ:	Spy49_0144(nga)

STZ:	SPYALAB49_000178

SPYH:

L897_00945

SPYO:

STAB901_00975

SEU:

SEQ_0311

SDS:	SDEG_2029(nga)

SDG:	SDE12394_10265

SDA:	GGS_1869(nga)

SDC:	SDSE_2125(nga)

SDQ:	SDSE167_2131(nga)

SCAI:

NCTC12191_01452

» show all

Reference

1 [PMID:4322210]

Authors

Fehrenbach FJ

Title

[Purification and crystallization of NAD glycohydrolase of C streptococci].

Journal

Eur J Biochem 18:94-102 (1971)
DOI:10.1111/j.1432-1033.1971.tb01219.x

Reference

2 [PMID:236282]

Authors

Grushoff PS, Shany S, Bernheimer AW

Title

Purification and properties of streptococcal nicotinamide adenine dinucleotide glycohydrolase.

Journal

J Bacteriol 122:599-605 (1975)
DOI:10.1128/JB.122.2.599-605.1975

Reference

3 [PMID:18952139]

Authors

Zhang L, Xu X, Luo Z, Shen D, Wu H

Title

Identification of an unusual AT(D)Pase-like activity in multifunctional NAD glycohydrolase from the venom of Agkistrodon acutus.

Journal

Biochimie 91:240-51 (2009)
DOI:10.1016/j.biochi.2008.09.003

Reference

4 [PMID:20018886]

Authors

Ghosh J, Anderson PJ, Chandrasekaran S, Caparon MG

Title

Characterization of Streptococcus pyogenes beta-NAD+ glycohydrolase: re-evaluation of enzymatic properties associated with pathogenesis.

Journal

J Biol Chem 285:5683-94 (2010)
DOI:10.1074/jbc.M109.070300

Reference

5 [PMID:21300288]

Authors

Smith CL, Ghosh J, Elam JS, Pinkner JS, Hultgren SJ, Caparon MG, Ellenberger T

Title

Structural basis of Streptococcus pyogenes immunity to its NAD+ glycohydrolase toxin.

Journal

Structure 19:192-202 (2011)
DOI:10.1016/j.str.2010.12.013

Other DBs

ExplorEnz - The Enzyme Database:

3.2.2.5

IUBMB Enzyme Nomenclature:

3.2.2.5

ExPASy - ENZYME nomenclature database:

3.2.2.5

BRENDA, the Enzyme Database:

3.2.2.5

CAS:

9025-46-1

DBGET integrated database retrieval system