KEGG   ENZYME: 3.3.2.14
Entry
EC 3.3.2.14                 Enzyme                                 
Name
2,4-dinitroanisole O-demethylase;
2,4-dinitroanisole ether hydrolase;
dnhA (gene name);
dnhB (gene name);
DNAN demethylase
Class
Hydrolases;
Acting on ether bonds;
Ether hydrolases
Sysname
2,4-dinitroanisole methanol hydrolase
Reaction(IUBMB)
2,4-dinitroanisole + H2O = methanol + 2,4-dinitrophenol [RN:R11189]
Reaction(KEGG)
R11189
Substrate
2,4-dinitroanisole [CPD:C21159];
H2O [CPD:C00001]
Product
methanol [CPD:C00132];
2,4-dinitrophenol [CPD:C02496]
Comment
The enzyme, characterized from the bacterium Nocardioides sp. JS1661, is involved in the degradation of 2,4-dinitroanisole. Unlike other known O-demethylases, such as EC 1.14.99.15, 4-methoxybenzoate monooxygenase (O-demethylating), or EC 1.14.11.32, codeine 3-O-demethylase, it does not require oxygen or electron donors, and produces methanol rather than formaldehyde.
History
EC 3.3.2.14 created 2015
Orthology
K20837  2,4-dinitroanisole O-demethylase
Reference
1  [PMID:25281383]
  Authors
Fida TT, Palamuru S, Pandey G, Spain JC
  Title
Aerobic biodegradation of 2,4-Dinitroanisole by Nocardioides sp. strain JS1661.
  Journal
Appl Environ Microbiol 80:7725-31 (2014)
DOI:10.1128/AEM.02752-14
  Sequence
[ag:AIQ77708 AIQ77709]
Other DBs
ExplorEnz - The Enzyme Database: 3.3.2.14
IUBMB Enzyme Nomenclature: 3.3.2.14
ExPASy - ENZYME nomenclature database: 3.3.2.14
BRENDA, the Enzyme Database: 3.3.2.14

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Chemical substance (4)   
   KEGG COMPOUND (4)   
Chemical reaction (3)   
   KEGG REACTION (1)   
   KEGG RCLASS (2)   
Gene (3)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (2)   
Protein sequence (15)   
   UniProt (13)   
   SWISS-PROT (2)   
DNA sequence (10)   
   GenBank (5)   
   EMBL (5)   
Protein domain (3)   
   InterPro (3)   
All databases (38)   

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