Hydrolases;
Acting on peptide bonds (peptidases);
Omega peptidases
Reaction(IUBMB)
Cleavage of C-terminal glycinamide from polypeptides
Comment
Inactivates vasopressin and oxytocin by splitting off glycinamide. Also cleaves ester substrates of trypsin and chymotrypsin. Although glycinamide is by far the preferred leaving group, other aminoacylamides may also be released, e.g. phenylalaninamide. The toad skin enzyme is inhibited by diisopropyl fluorophosphate.
History
EC 3.4.19.2 created 1978 as EC 3.4.15.2, transferred 1981 to EC 3.4.19.2
Reference
1
Authors
Fruhaufova, L., Suska-Brezezinska, E., Barth, T. and Rychlik, I.
Title
Rat liver enzyme inactivating oxytocin and its deamino-carba analogues.