KEGG ENZYME: 3.4.21.118

ENZYME: 3.4.21.118

Entry

EC 3.4.21.118               Enzyme

Name

kallikrein 8;
KLK8;
PRSS19;
human kallikrein 8;
hK8;
mK8;
ovasin;
tumor-associated differentially expressed gene 14;
TADG-14;
NP;
neuropsin

Class

Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases

Reaction(IUBMB)

Cleavage of amide substrates following the basic amino acids Arg or Lys at the P1 position, with a preference for Arg over Lys

Comment

The enzyme is activated by removal of an N-terminal prepropeptide [2,4]. The highest amidolytic activity is observed using Boc-Val-Pro-Arg!7-amido-4-methylcoumarin, which is a substrate of alpha-thrombin [2,4]. Substrates lacking basic amino acids in the P1 position are not cleaved [4]. The enzyme degrades casein, fibronectin, gelatin, collagen type IV, fibrinogen, and high-molecular-mass kininogen [3] and is associated with diseases such as ovarian cancer and Alzheimer's disease [4]. Belongs in peptidase family S1A.

History

EC 3.4.21.118 created 2006

Orthology

K08650

kallikrein 8

Genes

HSA:	11202(KLK8)

PTR:	456239(KLK8)

PPS:	100970185(KLK8)

PON:	100437370(KLK8)

NLE:	100587419(KLK8)

HMH:	116478139(KLK8)

MCC:	719488(KLK8)

MCF:	102143235(KLK8)

MTHB:

126943165

MNI:	105497131(KLK8)

CSAB:

103235109(KLK8)

CATY:

105595348(KLK8)

PANU:

101008136(KLK8)

TGE:	112612813(KLK8)

MLEU:

105528931(KLK8)

RRO:	104667249(KLK8)

RBB:	108532406(KLK8)

TFN:	117064577 117091658(KLK8)

PTEH:

111520135(KLK8)

CANG:

105514719(KLK8)

CJC:	100404633(KLK8)

SBQ:	101047321(KLK8)

CIMI:

108291977(KLK8)

CSYR:

103274732(KLK8)

MMUR:

105865553(KLK8)

LCAT:

123623762(KLK8)

PCOQ:

105809350(KLK8)

OGA:	105888334(KLK8)

MMU:	259277(Klk8)

MCAL:

110297556(Klk8)

MPAH:

110320329(Klk8)

RNO:	308565(Klk8)

MCOC:

116102105(Klk8)

ANU:	117711196(Klk8)

MUN:	110553717(Klk8)

CGE:

100752953

MAUA:

101839688(Klk8)

PROB:

127213894(Klk8)

PLEU:

114683940(Klk8)

MORG:

121448412

MFOT:

126488150

NGI:	103743767(Klk8)

HGL:	101715440(Klk8)

CPOC:

100716239

CCAN:

109683871(Klk8)

DORD:

105994286(Klk8)

DSP:	122125632(Klk8)

PLOP:

125368109

NCAR:

124967154

MMMA:

107151203(Klk8)

OPI:	101523645(KLK8)

TUP:

102468122

GVR:	103597721(KLK8)

CFA:	484352(KLK8)

CLUD:

112645077(KLK8)

VVP:	112931314(KLK8)

VLG:

121484998

NPO:	129500971(KLK8)

AML:	100476177(KLK8)

UMR:	103657288(KLK8)

UAH:	113247321(KLK8)

UAR:	123778002(KLK8)

ELK:

111160641

LLV:

125089997

MPUF:

101689273(KLK8)

MLK:	131817585(KLK8)

NVS:

122911770

ORO:

101383132

EJU:	114199519(KLK8)

ZCA:	113936323(KLK8)

MLX:	117997972(KLK8)

NSU:	110572720(KLK8)

LWW:

102727224

FCA:	101090308(KLK8)

PYU:	121018991(KLK8)

PCOO:

112851203

PBG:	122493798(KLK8)

PVIV:

125153162(KLK8)

LRUF:

124510865

PTG:	102964756(KLK8)

PPAD:

109252984 109252986(KLK8)

PUC:

125915719

AJU:

106989678

HHV:	120244404(KLK8)

BTA:	618438(KLK8)

BOM:	102281911(KLK8)

BIU:	109571931(KLK8)

BBUB:

102402053(KLK8)

BBIS:

104995699

CHX:	102178849(KLK8)

OAS:	101112755(KLK8)

BTAX:

128063379(KLK8)

ODA:	120872028(KLK8)

CCAD:

122421606(KLK8)

MBEZ:

129546485(KLK8)

SSC:	110261027(KLK8)

CFR:

102521031

CBAI:

105062681(KLK8)

CDK:

105100154

VPC:	102529503(KLK8)

BMUS:

118885405

ECB:	100067301(KLK8)

EPZ:	103567751(KLK8)

EAI:	106847546(KLK8)

MYB:	102241466(KLK8)

MYD:	102764579(KLK8)

MMYO:

118649163(KLK8)

PKL:	118714912(KLK8)

EFUS:

103297597(KLK8)

MNA:	107531775(KLK8)

DRO:

112310176

AJM:

119052030

PDIC:

114510587

PHAS:

123830316

MMF:	118638351(KLK8)

PPAM:

129082708(KLK8)

HAI:	109391034(KLK8)

RFQ:	117035627(KLK8)

PALE:

102893463

PGIG:

120584034(KLK8)

PVP:

105307411

RAY:	107500461(KLK8)

TOD:

119249655

SARA:

101554967(KLK8)

LAV:

100659511

TMU:

101340570

ETF:

101640603

DNM:

101438195

MDO:	103092918(KLK8)

GAS:	123240781(KLK8)

SHR:	100933656(KLK8)

AFZ:

127556450

PCW:	110220141(KLK8)

OAA:	114811990 114812093(KLK8)

CJO:

107307352

NMEL:

110391482

OMA:

130262827

PCRI:

104025168

PADL:

103925967

AFOR:

103905999

BREG:

104631035

FGA:

104078072

NNT:

104403169

SHAB:

115603372 115603373

ACAR:

104531576

CVF:

104282761

RTD:

128901569

AROW:

112962207

NCC:

104941648

MSAM:

119898609 119898610

ONL:	109199930 109199932 109202569 109202581

OAU:	116329159 120440772

XMA:	102227215 102230174

XCO:

114140853

PRET:

103473950

PLAI:

106962246

AOCE:

111577596

LCF:

108889075

SDU:

111230926

SLAL:

111671872

SASA:

106564436 106594015 106596927

OMY:	110494920 118940083

OKE:

118376301

ARUT:

117964822

CIN:

100177494

FAS:

105266625

AGB:

108909605

PGW:

126372249

PSOJ:

PHYSODRAFT_393864

» show all

Reference

1 [PMID:7623137]

Authors

Chen ZL, Yoshida S, Kato K, Momota Y, Suzuki J, Tanaka T, Ito J, Nishino H, Aimoto S, Kiyama H, et al.

Title

Expression and activity-dependent changes of a novel limbic-serine protease gene in the hippocampus.

Journal

J Neurosci 15:5088-97 (1995)
DOI:10.1523/JNEUROSCI.15-07-05088.1995

Sequence

[mmu:259277]

Reference

2 [PMID:9556608]

Authors

Shimizu C, Yoshida S, Shibata M, Kato K, Momota Y, Matsumoto K, Shiosaka T, Midorikawa R, Kamachi T, Kawabe A, Shiosaka S.

Title

Characterization of recombinant and brain neuropsin, a plasticity-related serine protease.

Journal

J Biol Chem 273:11189-96 (1998)
DOI:10.1074/jbc.273.18.11189

Sequence

[mmu:259277]

Reference

3 [PMID:16337200]

Authors

Rajapakse S, Ogiwara K, Takano N, Moriyama A, Takahashi T

Title

Biochemical characterization of human kallikrein 8 and its possible involvement in the degradation of extracellular matrix proteins.

Journal

FEBS Lett 579:6879-84 (2005)
DOI:10.1016/j.febslet.2005.11.039

Sequence

[hsa:11202]

Reference

4 [PMID:16800733]

Authors

Kishi T, Cloutier SM, Kundig C, Deperthes D, Diamandis EP.

Title

Activation and enzymatic characterization of recombinant human kallikrein 8.

Journal

Biol Chem 387:723-31 (2006)
DOI:10.1515/BC.2006.091

Other DBs

ExplorEnz - The Enzyme Database:

3.4.21.118

IUBMB Enzyme Nomenclature:

3.4.21.118

ExPASy - ENZYME nomenclature database:

3.4.21.118

BRENDA, the Enzyme Database:

3.4.21.118

CAS:	171715-15-4

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Gene (224)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (182)   
   KEGG MGENES (1)   
   RefGene (40)   
Protein sequence (26)   
   UniProt (3)   
   SWISS-PROT (3)   
   RefSeq(pep) (11)   
   PDBSTR (9)   
DNA sequence (13)   
   RefSeq(nuc) (12)   
   GenBank (1)   
3D Structure (2)   
   PDB (2)   
Protein domain (6)   
   InterPro (6)   
All databases (271)   

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