KEGG   ENZYME: 3.4.21.123
Entry
EC 3.4.21.123               Enzyme                                 
Name
kumamolysin;
KSCP;
kumamolisin
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases
Reaction(IUBMB)
The enzyme preferentially hydrolyses peptides having an Ala or Pro residue at P2 position and prefers such charged amino acid residues as Glu or Arg at the P2' position. In the oxidized insulin B chain, kumamolysin preferentially cleaves between Leu15 and Tyr16
Comment
This bacterial pepstatin-insensitive carboxyl proteinase has been isolated and characterized from Bacillus sp. MN-32 and from several Burkholderia spp. Kumamolysin from Bacillus sp. MN-32 exhibits a Ser278/Glu78/Asp82 catalytic triad. The enzyme is a type example of peptidase family S53 in the MEROPS Peptidas Database.
History
EC 3.4.21.123 created 2023
Orthology
K08677  kumamolisin
Genes
ECA: ECA1450
PATR: EV46_07340
PATO: GZ59_14890
PCT: PC1_1326
PCC: PCC21_013560
PWA: Pecwa_3008
PPAR: A8F97_03625
PEC: W5S_2993
PWS: A7983_15765
PPOA: BJK05_01830
PBRA: B5S52_14895
PPUJ: E2566_07290
PARI: I2D83_15380
PAQU: DMB82_0009450 DMB82_0013710
PVZ: OA04_15260
PPAV: LOZ86_07170
PQU: IG609_011875 IG609_013500
PPOO: LW347_07245
PCAC: OI450_08435
PACB: M9782_09205
SLIG: GTU79_06275
XTW: AB672_04540
XCV: XCV2734(kscP)
XAX: XACM_2518
XAC: XAC2537
XCI: XCAW_02215
XCT: J151_02716
XCJ: J158_02703
XCU: J159_02701
XCN: J169_02713
XCW: J162_02705
XCR: J163_02700
XCM: J164_02702
XCF: J172_02707
XFU: XFF4834R_chr25560(kscP)
XAO: XAC29_12915
XOM: XOO2970(XOO2970)
XOO: XOO3125
XOP: PXO_01452
XOY: AZ54_08300
XOR: XOC_1923
XOZ: BE73_09005
XTN: FD63_05980
XFR: BER92_06280
XVE: BJD12_03695
XPE: BJD13_03795
XHR: XJ27_06550
XGA: BI317_10120
XPH: XppCFBP6546_19740(XppCFBP6546P_19740)
XVA: C7V42_09500
XAN: AC801_09500
XTH: G4Q83_12095
XDY: NYR95_13900
RGL: CS053_02590 CS053_04830
RTH: LRK53_03680
DJI: CH75_01975 CH75_18110
DJA: HY57_04900 HY57_07375
DTX: ATSB10_38090
DYE: EO087_13995
DCS: ISN74_01065
DTL: H8F01_11655
DTA: DYST_03434
MPSY: CEK71_17210
MME: Marme_2333
CVI: CV_3519
CVC: BKX93_08350
CHRO: CXB49_03945
CHRI: DK842_02520
CHRB: DK843_08000
RMN: TK49_21180
RWE: KOL96_02205
REU: Reut_A1759
BMA: BMAA1568
BML: BMA10229_2032
BMN: BMA10247_A0713
BMAL: DM55_4895(scpA)
BMAE: DM78_4143(scpA)
BMAQ: DM76_3849(scpA)
BMAI: DM57_08135
BMAF: DM51_3270(scpA)
BMAZ: BM44_5025(scpA)
BMAB: BM45_3925(scpA)
BPS: BPSS1562
BPM: BURPS1710b_A0608(scpA)
BPL: BURPS1106A_A2116
BPD: BURPS668_A2202
BPSE: BDL_4911(scpA)
BPSM: BBQ_4586(scpA)
BPSU: BBN_5008(scpA)
BPSD: BBX_4072(scpA)
BPZ: BP1026B_II1662
BPQ: BPC006_II2097
BPK: BBK_4213(scpA)
BPSH: DR55_3713(scpA)
BPSA: BBU_4471(scpA)
BPSO: X996_3498(kscp)
BUT: X994_5553(scpA)
BTE: BTH_II0811
BTQ: BTQ_4098(kscp)
BTJ: BTJ_5130(kscp)
BTZ: BTL_3601(kscp)
BTD: BTI_4041(scpA)
BTV: BTHA_4286(kscp)
BTHE: BTN_5584(kscp)
BTHM: BTRA_3592(kscp)
BTHA: DR62_5765
BTHL: BG87_3554(kscp)
BOK: DM82_5685(kscp)
BOC: BG90_4947(kscp)
BUU: WS70_25050
BSAV: WS86_26695
BHG: I6G56_20180
BUD: AQ610_22965
BVI: Bcep1808_3532
BVE: AK36_3234(scpA)
BUR: Bcep18194_B0370
BCM: Bcenmc03_4988
BCH: Bcen2424_5280
BCJ: BCAM2478
BCEN: DM39_5365(kscp)
BCEW: DM40_3128(kscp)
BCEO: I35_6377
BAM: Bamb_4640
BAC: BamMC406_5169
BMJ: BMULJ_05075
BMU: Bmul_3441
BMK: DM80_4829(scpA)
BMUL: NP80_5103(scpA)
BCT: GEM_3200
BCED: DM42_5501(scpA)
BCEP: APZ15_22920
BDL: AK34_3355(scpA)
BPYR: ABD05_23190
BCON: NL30_00165
BUB: BW23_3698(kscp) BW23_5311(kscp)
BDF: WI26_25200
BLAT: WK25_28220
BTEI: WS51_09335
BSEM: WJ12_32420
BPSL: WS57_01755
BMEC: WJ16_30895
BSTG: WT74_31270
BSTL: BBJ41_26335
BANN: JFN94_16710
BARI: NLX30_24745
BAEN: L3V59_27325
BGL: bglu_2g04140
BGU: KS03_5501(kscp)
BUG: BC1001_5482
BGF: BC1003_3923
BGD: bgla_2g05800
BGO: BM43_4218(kscp)
BYI: BYI23_B009810
BUK: MYA_3158
BUE: BRPE67_BCDS13930
BUL: BW21_6049(scpA)
BUQ: AC233_22570
BGP: BGL_2c05040
BPLA: bpln_2g05540
BUM: AXG89_18245
BUI: AX768_28020
BURK: DM992_24030
BXE: Bxe_B1994
BXB: DR64_7295(kscp)
BPH: Bphy_5054 Bphy_6936
BGE: BC1002_3613
BPX: BUPH_05604
BPY: Bphyt_4778
BUZ: AYM40_24010
BFN: OI25_4345(kscp)
BCAI: K788_0008317
PSPW: BJG93_17505
PARA: BTO02_27645
PARB: CJU94_20275
PHS: C2L64_27810 C2L64_47190
PTER: C2L65_20690
PGP: CUJ91_20950
PCJ: CUJ87_19010
PTS: CUJ90_28420
PCAF: DSC91_001830
PMEG: FNZ07_09235
PTRO: G5S35_14860
PGIS: I6I06_20665
PACP: FAZ97_20720
PACS: FAZ98_16930
PEW: KZJ38_30250 KZJ38_32385
PDIO: PDMSB3_1164.1
PBRY: NDK50_30845
PSAA: QEN71_21205
PKF: RW095_09815
PPAI: E1956_17385
BRH: RBRH_00732
PTX: ABW99_09225
CABA: SBC2_67730(pcp_2)
BUO: BRPE64_BCDS03210
CABK: NK8_32120
TVL: FAZ95_26935 FAZ95_29770
TCAR: U0034_28745
BBRO: BAU06_17560
BFZ: BAU07_08685
BOH: AKI39_16825
PARK: LSG25_15740
CFU: CFU_0252(kscP)
DUG: HH213_05705
TMJ: P0M04_18310
RLG: Rleg_5453
BJA: blr0694(blr0694)
BJU: BJ6T_06670
BJP: RN69_03275
AOL: S58_72070
BRK: CWS35_35135
BGQ: X265_00750
BGZ: XH91_30770
BBET: F8237_15165
BARH: WN72_09555
BQB: J4P68_0010135
BBAN: J4G43_000875
BGK: IC762_27960
BCOU: IC761_00740 IC761_32325
MSL: Msil_3227 Msil_3445
MTUN: MTUNDRAET4_0320.1 MTUNDRAET4_0937
MHEY: H2LOC_002795 H2LOC_008770 H2LOC_013100 H2LOC_013110 H2LOC_018895
MPAR: F7D14_21455
CAUL: KCG34_00085
SMOR: LHA26_16190
POQ: KZX46_00815 KZX46_10500
NCH: A0U93_08845
LCK: HN018_01940
THAC: CSC3H3_22225
CCX: COCOR_04619(pcp) COCOR_06298
AGE: AA314_02167
CFUS: CYFUS_000841
SCL: sce0499 sce0542 sce7535
SCU: SCE1572_03505 SCE1572_43575
CCRO: CMC5_070950
PAUU: E8A73_007470 E8A73_041055 E8A73_042160
MRM: A7982_07458
SAMY: DB32_005095
HOH: Hoch_6805
PUK: PU629_12425
AAC: Aaci_0057 Aaci_0247 Aaci_0759
AAD: TC41_0074 TC41_0325 TC41_0737
AFX: JZ786_01045 JZ786_17585
AACO: K1I37_00995
ACUR: JZ785_00085
ADAU: NZD86_21810
AFAS: NZD89_01060 NZD89_22520
ACYC: JI721_10925
LBH: Lbuc_0123 Lbuc_0836 Lbuc_1244 Lbuc_2221
LBN: LBUCD034_0167 LBUCD034_0897 LBUCD034_1368 LBUCD034_2269 LBUCD034_2328
LPAR: FAM21731_01174 FAM21731_02128 FAM21731_02219
LCU: PL11_000195 PL11_002165 PL11_006755 PL11_008250
LKF: DNL43_04115 DNL43_05695 DNL43_06405 DNL43_10675
LHIL: G8J22_00453(pcp_1) G8J22_00552 G8J22_00553 G8J22_00554 G8J22_00556 G8J22_01663(pcp_2)
LBR: LVIS_0233
LBK: LVISKB_0233
LKO: ABN16_06985
LKU: APS55_07095
SAY: TPY_2558 TPY_3226
SAP: Sulac_1687 Sulac_3308
STHR: BXT84_09280 BXT84_14850
MSA: Mycsm_00302 Mycsm_06332
MYV: G155_08230
MYE: AB431_20580 AB431_26075 AB431_28190 AB431_28500
MDX: BTO20_27280
MGAU: MGALJ_45780 MGALJ_50630
MPAA: MKK62_13035
MADI: A7U43_08855
MWU: PT015_06440
MSM: MSMEG_1617
MSG: MSMEI_1579
MSB: LJ00_08080
MSN: LI99_08080
MSH: LI98_08080
MVA: Mvan_5919
MCB: Mycch_1692
MNE: D174_07565
MYN: MyAD_07430
MFT: XA26_16410
MPHL: MPHLCCUG_04525(pcp_1) MPHLCCUG_04968(pcp_2)
MVQ: MYVA_5875
MLL: B1R94_24685 B1R94_27820
MRH: MycrhN_1164 MycrhN_1647
MHAS: MHAS_04374(pcp_1) MHAS_04847(pcp_2)
MAUU: NCTC10437_02348(pcp)
MMAG: MMAD_12750
MMOR: MMOR_03400 MMOR_58530
MAIC: MAIC_01290 MAIC_01900 MAIC_06240 MAIC_27570
MALV: MALV_22660
MTY: MTOK_27540
MPSC: MPSYJ_29750
MARZ: MARA_26480
MGAD: MGAD_44130 MGAD_49050
MHEV: MHEL_02680 MHEL_23640 MHEL_24210 MHEL_28670 MHEL_42510 MHEL_51310
MSAR: MSAR_13920 MSAR_40610 MSAR_48690
MANY: MANY_03910 MANY_36590 MANY_40850
MAUB: MAUB_19920 MAUB_24820
MPOF: MPOR_08590
MPHU: MPHO_45750 MPHO_50410
MMUC: C1S78_021935 C1S78_024395
MMAT: MMAGJ_44890
MBOK: MBOE_59770
MFG: K6L26_24425
MSEI: MSEDJ_25180 MSEDJ_48310
MFLV: NCTC10271_04497(pcp_1) NCTC10271_04975(pcp_2)
MCEE: MCEL_27270
MGO: AFA91_30840
MHOL: K3U96_01140 K3U96_23210
MSEN: K3U95_07625
MPAE: K0O64_03030 K0O64_19860 K0O64_28285 K0O64_28615
MRF: MJO55_17115
MDF: K0O62_07130
MCRO: MI149_03620 MI149_20735 MI149_28100 MI149_28400
MGRO: FZ046_11990
CCYC: SCMU_02630
NTP: CRH09_33730
SRT: Srot_1146 Srot_1399
SALB: XNR_3232
SCI: B446_01360 B446_33930
SLC: SL103_07790
SGS: AVL59_35650
SKY: D0C37_15255
SLS: SLINC_1203
SALU: DC74_1306
SALL: SAZ_07025
SPUN: BFF78_37455
SGV: B1H19_33730
STRO: STRMOE7_12250
SGE: DWG14_05819(pcp_3)
SVR: CP971_33170
SAUH: SU9_009245
SINE: KI385_14070
SNIG: HEK616_12060
SENG: OJ254_16680
KAU: B6264_26260
KIT: CFP65_0186
LEIF: HF024_12925
LSE: F1C12_21420
MRG: SM116_13620
FRP: AX769_19500
FSB: GCM10025867_40980
HUW: FPZ11_06935
GRY: D7I44_04010
GLN: F1C58_07605
SUBA: LQ955_10365
ARE: AL755_11015
ARTP: E5206_14260
APOA: J0916_02975
AWW: G8758_07265
ARI: UM93_08605
SATK: SA2016_0232
ALX: LVQ62_05335
MIK: FOE78_11255
MICG: GJV80_11065
NBE: Back2_02110
FAL: FRAAL0791 FRAAL2666
NAKA: H7F38_17605
AMD: AMED_8435
AMN: RAM_43305
AMM: AMES_8306
AMZ: B737_8307
AMYC: CU254_21220
AACD: LWP59_08855
KAL: KALB_1292 KALB_1805 KALB_3044 KALB_7080
KUT: JJ691_95130
ACTL: L3i22_047660
PFLA: Pflav_011310
ATL: Athai_01130
ASER: Asera_06370
JTL: M6D93_00400
JCY: M6B22_10590
BALA: DSM104299_00728
AFO: Afer_1071
GLJ: GKIL_0505 GKIL_0994 GKIL_1184 GKIL_1941 GKIL_2512
KBS: EPA93_15935 EPA93_43165
KBB: ccbrp13_08970
DEZ: DKM44_03495
DMB: E5F05_00175
FGI: OP10G_0986
VAB: WPS_02570
SNEP: Enr13x_12180(pcp_1)
FTJ: FTUN_3290
PBOR: BSF38_00505(pcp_1)
AGV: OJF2_32260(pcp_1)
ABA: Acid345_2635
ACA: ACP_1810
ACM: AciX9_0612
GMA: AciX8_0674
GRW: FTO74_03390
GRB: GOB94_05560
TALB: FTW19_03130
ABAS: ACPOL_5025
EDA: GWR55_09285
EDG: H7846_04770
ADIN: H7849_19685
SUS: Acid_0578
ATEE: K9M52_18515
MGIN: FRZ54_11885
MROB: HH214_11335
MJJ: PQO05_20230
SLI: Slin_2888
SRD: SD10_15160
SPIR: CWM47_01100
SRHO: HH216_10620
HYP: A0257_14965
HYZ: AXW84_01105
HNV: DDQ68_16760
KOS: KORDIASMS9_00547(pcp)
TTN: TTX_1946
TUZ: TUZN_1547
VMO: VMUT_0742
 » show all
Reference
1  [PMID:8416942]
  Authors
Murao S, Ohkuni K, Nagao M, Hirayama K, Fukuhara K, Oda K, Oyama H, Shin T.
  Title
Purification and characterization of kumamolysin, a novel thermostable pepstatin-insensitive carboxyl proteinase from Bacillus novosp. MN-32.
  Journal
J Biol Chem 268:349-55 (1993)
Reference
2  [PMID:10965051]
  Authors
Oda K, Ogasawara S, Oyama H, Dunn BM.
  Title
Subsite preferences of pepstatin-insensitive carboxyl proteinases from prokaryotes: kumamolysin, a thermostable pepstatin-insensitive carboxyl proteinase.
  Journal
J Biochem 128:499-507 (2000)
DOI:10.1093/oxfordjournals.jbchem.a022780
Reference
3  [PMID:11983085]
  Authors
Oyama H, Hamada T, Ogasawara S, Uchida K, Murao S, Beyer BB, Dunn BM, Oda K.
  Title
A CLN2-related and thermostable serine-carboxyl proteinase, kumamolysin: cloning, expression, and identification of catalytic serine residue.
  Journal
J Biochem 131:757-65 (2002)
DOI:10.1093/oxfordjournals.jbchem.a003162
  Sequence
[ag:BAB85637]
Reference
4  [PMID:12057200]
  Authors
Comellas-Bigler M, Fuentes-Prior P, Maskos K, Huber R, Oyama H, Uchida K, Dunn BM, Oda K, Bode W.
  Title
The 1.4 a crystal structure of kumamolysin: a thermostable serine-carboxyl-type proteinase.
  Journal
Structure 10:865-76 (2002)
DOI:10.1016/s0969-2126(02)00772-4
  Sequence
[ag:BAB85637]
Reference
5  [PMID:15014068]
  Authors
Wlodawer A, Li M, Gustchina A, Tsuruoka N, Ashida M, Minakata H, Oyama H, Oda K, Nishino T, Nakayama T.
  Title
Crystallographic and biochemical investigations of kumamolisin-As, a serine-carboxyl peptidase with collagenase activity.
  Journal
J Biol Chem 279:21500-10 (2004)
DOI:10.1074/jbc.M401141200
Other DBs
ExplorEnz - The Enzyme Database: 3.4.21.123
IUBMB Enzyme Nomenclature: 3.4.21.123
ExPASy - ENZYME nomenclature database: 3.4.21.123
BRENDA, the Enzyme Database: 3.4.21.123

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Gene (577)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (511)   
   KEGG MGENES (65)   
All databases (577)   

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