KEGG ENZYME: 3.4.21.25

ENZYME: 3.4.21.25

Entry

EC 3.4.21.25                Enzyme

Name

cucumisin;
euphorbain;
solanain;
hurain;
tabernamontanain

Class

Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases

Reaction(IUBMB)

Hydrolysis of proteins with broad specificity

Comment

From the sarcocarp of the musk melon (Cucumis melo). In peptidase family S8 (subtilisin family). Other endopeptidases from plants, which are less well characterized but presumably of serine-type, include euphorbain from Euphorbia cerifera [6], solanain from horse-nettle Solanum elaeagnifolium [1], hurain from Hura crepitans [2] and tabernamontanain from Tabernamontana grandiflora [3].

History

EC 3.4.21.25 created 1978 (EC 3.4.21.56 created 1972 as EC 3.4.99.7 transferred 1989 to EC 3.4.21.56, deleted 1992, EC 3.4.99.9 created 1972 deleted 1992, EC 3.4.99.21 created 1972 deleted 1992, EC 3.4.99.23 created 1972 deleted 1992, all covered by EC 3.4.21.25)

Reference

Authors

Greenberg, D.M. and Winnick, T.

Title

Plant proteases. I. Activation-inhibition reactions.

Journal

J Biol Chem 135:761-773 (1940)

Reference

Authors

Jaffe, W.G.

Title

Hurain, a new plant protease from Hura crepitans.

Journal

J Biol Chem 149:1-7 (1943)

Reference

Authors

Jaffe, W.G.

Title

A new vegetable proteolytic enzyme of the papain class.

Journal

Rev Brasil Biol 3:149-157 (1943)

Reference

4 [PMID:5423]

Authors

Kaneda M, Tominaga N.

Title

Isolation and characterization of a proteinase from the sarcocarp of melon fruit.

Journal

J Biochem (Tokyo) 78:1287-96 (1975)
DOI:10.1093/oxfordjournals.jbchem.a131026

Reference

5 [PMID:6427203]

Authors

Kaneda M, Ohmine H, Yonezawa H, Tominaga N.

Title

Amino acid sequence around the reactive serine of cucumisin from melon fruit.

Journal

J Biochem (Tokyo) 95:825-9 (1984)
DOI:10.1093/oxfordjournals.jbchem.a134674

Reference

Authors

Lynn, K.R. and Clevette-Radford, N.A.

Title

Two proteases from the latex of Elaeophorbia drupifera.

Journal

Phytochemistry 24:2843-2845 (1985)

Reference

Authors

Kaneda, N., Minematsu, Y., Powers, J.C. and Tominaga, N.

Title

Specificity of cucumisin in hydrolysis of peptide thiobenzyl esters.

Journal

Agric Biol Chem 50:1075-1076 (1986)

Other DBs

ExplorEnz - The Enzyme Database:

3.4.21.25

IUBMB Enzyme Nomenclature:

3.4.21.25

ExPASy - ENZYME nomenclature database:

3.4.21.25

BRENDA, the Enzyme Database:

3.4.21.25

CAS:	82062-89-3

All links

Protein sequence (273)   
   UniProt (266)   
   SWISS-PROT (1)   
   RefSeq(pep) (2)   
   PDBSTR (4)   
DNA sequence (41)   
   RefSeq(nuc) (3)   
   GenBank (31)   
   EMBL (7)   
3D Structure (2)   
   PDB (2)   
Protein domain (9)   
   InterPro (9)   
All databases (325)   

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