KEGG ENZYME: 3.4.21.62

ENZYME: 3.4.21.62

Entry

EC 3.4.21.62                Enzyme

Name

subtilisin;
alcalase;
alcalase 0.6L;
alcalase 2.5L;
ALK-enzyme;
bacillopeptidase A;
bacillopeptidase B;
Bacillus subtilis alkaline proteinase bioprase;
bioprase AL 15;
bioprase APL 30;
colistinase;
(see also comments);
subtilisin J;
subtilisin S41;
subtilisin Sendai;
subtilisin GX;
subtilisin E;
subtilisin BL;
genenase I;
esperase;
maxatase;
thermoase PC 10;
protease XXVII;
thermoase;
superase;
subtilisin DY;
subtilopeptidase;
SP 266;
savinase 8.0L;
savinase 4.0T;
kazusase;
protease VIII;
opticlean;
Bacillus subtilis alkaline proteinase;
protin A 3L;
savinase;
savinase 16.0L;
savinase 32.0 L EX;
orientase 10B;
protease S

Class

Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases

Reaction(IUBMB)

Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyses peptide amides

Comment

Subtilisin is a serine endopeptidase, type example of peptidase family S8. It contains no cysteine residues (although these are found in homologous enzymes). Species variants include subtilisin BPN' (also subtilisin B, subtilopeptidase B, subtilopeptidase C, Nagarse, Nagarse proteinase, subtilisin Novo, bacterial proteinase Novo) and subtilisin Carlsberg (subtilisin A, subtilopeptidase A, alcalase Novo). Similar enzymes are produced by various Bacillus subtilis strains and other Bacillus species [1,3]

History

EC 3.4.21.62 created 1992 (EC 3.4.21.14 created 1961 as EC 3.4.4.16, transferred 1972 to EC 3.4.21.14, modified 1986, part incorporated 1992)

Orthology

K01342

subtilisin

Genes

VNA:	PN96_21170

VEJ:	VEJY3_20426

VZI:	G5S32_16115

PAZO:

AYR47_08420

PPUU:

PputUW4_00217

PFZ:	AV641_21465

PPSY:

AOC04_18875

PVK:	EPZ47_01330

PBZ:	GN234_16695

PAGR:

E2H98_07725

SPSW:

Sps_01013

PSPO:

PSPO_b1036

PAEW:

KIH87_09980

MII:	BTJ40_12175

MICT:

FIU95_10505(isp)

MDN:	JT25_023420

METL:

U737_14160

GAI:	IMCC3135_18295(aprE)

TTC:	FOKN1_1929

TOL:

TOL_3513

TOR:	R615_16425

AHA:

AHA_3478

AHY:	AHML_18455

AHD:	AI20_01980

AHR:	V428_19170

AHP:	V429_19200

AHJ:	V469_04105

AHH:	RY45_17885

ADH:	CK627_08380

CTI:	RALTA_A2222

PTER:

C2L65_19390

PUS:	CKA81_09145

CDN:	BN940_13916

HPSE:

HPF_14095

PBH:	AAW51_0831

JAJ:	EKL02_11675

MTIM:

DIR46_22305

MASY:

DPH57_21545

MASZ:

C9I28_00125

MPLI:

E1742_17405

DUG:	HH213_00655

ARES:

IWH25_04775

MLN:	A9174_32325

RJG:	CCGE525_36295 CCGE525_37610

RGR:	FZ934_20880

AOL:

S58_68990

BRQ:	CIT40_26285

BGZ:	XH91_13745

BSYM:

CIT39_25275

MEE:	DA075_32105

MSC:

BN69_1924

MROS:

EHO51_15640

DSH:

Dshi_0257

MALG:

MALG_02172

PHAO:

HF685_13840

ERK:	CD351_04895

ERF:	FIU90_00740(apr1)

SHUM:

STHU_11850

ALI:	AZOLI_p20735

DEX:	HWD60_18910

GSK:	KN400_2097

GPI:	GPICK_05335

GAO:	A2G06_06505

GEM:

GM21_1195

GBM:

Gbem_3055

PACE:

A6070_09530

DML:	Dmul_39070

DMM:	dnm_002940

BSU:	BSU10300(aprE)

BSR:

I33_1162

BSL:

A7A1_3704

BSH:	BSU6051_10300(aprE)

BSY:	I653_05205 I653_16335

BSUT:

BSUB_01127(aprE)

BSUL:

BSUA_01127(aprE)

BSUS:

Q433_05825

BSO:	BSNT_07467(aprN)

BSN:	BSn5_17030

BSQ:	B657_10300(aprE)

BSX:	C663_1054(aprE) C663_3243(aprX)

BSP:	U712_05250

BSS:	BSUW23_05210(aprE)

BST:

GYO_1328

BIT:	BIS30_15855

BLI:	BL01111(apr)

BLD:	BLi01109(apr)

BLH:	BaLi_c12310(aprE)

BAY:	RBAM_010500

BAQ:	BACAU_1010(aprE)

BYA:	BANAU_0954(aprE)

BAMP:

B938_05060

BQY:	MUS_1081(aprE)

BAML:

BAM5036_0953(aprE)

BAMA:

RBAU_1012(aprE)

BAMN:

BASU_0989(aprE)

BAMB:

BAPNAU_2748(aprE)

BAMT:

AJ82_05900

BAMY:

V529_09920

BMP:	NG74_01058(apr)

BAO:	BAMF_1119(aprE)

BAZ:	BAMTA208_04835(aprE)

BQL:	LL3_01118(aprE)

BXH:	BAXH7_01013(apr)

BAMI:

KSO_014610

BAMC:

U471_10430

BAMF:

U722_05330

BSIA:

CWD84_16485

BAE:	BATR1942_02760

BVM:	B9C48_05260

BSON:

S101395_03724

BHT:	DIC78_04470

BMOJ:

HC660_10860(aprE)

BTEQ:

G4P54_05465(aprE)

BIQ:	AN935_05370

BSTR:

QI003_05605(aprE)

BCX:

BCA_4942

BCY:	Bcer98_3892

BTL:

BALH_4378

BWE:	BcerKBAB4_5739

BPU:

BPUM_0972

BPUM:

BW16_05260 BW16_11335

BPUS:

UP12_02845 UP12_05210 UP12_10600 UP12_18400

BIF:	N288_23435

BGY:	BGLY_1146(apr) BGLY_1970

BWH:	A9C19_20320

BXI:	BK049_03075 BK049_08985 BK049_16485

BALT:

CFN77_05165 CFN77_11185

BAER:

BAE_12730 BAE_18345

BSAF:

BSL056_05155 BSL056_11625

BSHI:

LGQ02_01210

BCAB:

EFK13_05820(aprE)

BRY:	M0696_05775(aprE)

BJS:

MY9_1131

GST:	HW35_12820

BACW:

QR42_05055 QR42_10555

BACP:

SB24_04625

BACB:

OY17_07935

BACY:

QF06_04075

BACL:

BS34A_11440(aprE)

BALM:

BsLM_1072

BACS:

AUL54_05350

BZH:	NF868_04460 NF868_09465

BAEI:

RE735_09290 RE735_14380

BHK:	B4U37_08185 B4U37_08190

BCOH:

BC6307_03110 BC6307_09490 BC6307_20400

BMQ:	BMQ_pBM60006

BMH:	BMWSH_p211(alp)

BMEG:

BG04_5989

BFX:	BC359_19465 BC359_20835 BC359_20895 BC359_20955

PKS:	IE339_22580

BHA:	BH0684(alp) BH0855

BCL:	ABC0761(aprE)

BLE:	BleG1_0395 BleG1_3277

SHUA:

PQ477_04010 PQ477_10340

BGI:	BGM20_20425

BPF:	BpOF4_09070(subE) BpOF4_12080

BON:	A361_05130 A361_05175

BKO:	CKF48_03925

CGOT:

J1899_03915 J1899_20685

CSUA:

IM538_05940

CFIR:

NAF01_04805

CSPG:

LS684_19025

CPSS:

M5V91_16640

GLI:	GLN3_08620

GTN:

GTNG_3101

GJF:	M493_16490

GSR:	GS3922_00340

PCAL:

BV455_02091(aprE)

ANM:	GFC28_2287

ANL:	GFC29_3149

AND:	GRQ40_04675

LSP:

Bsph_0341

LGY:	T479_08835 T479_19860

LFU:	HR49_04065

HMN:	HM131_05010

HLI:

HLI_15035

HAMY:

MUO15_14510

VIG:	BKP57_02100 BKP57_07055

FAR:	ABE41_017340 ABE41_019275

APAK:

AP3564_01855

ACOP:

RI196_07180

BMUR:

ABE28_015170

PASA:

BAOM_2408(aprE)

PPSR:

I6J18_07910 I6J18_17480

PSYH:

D0S48_07750

PSYO:

PB01_03765

POF:	GS400_07575

PCHU:

QNI29_08520

MDG:	K8L98_08770

AIA:	AWH56_020315

PSUA:

FLK61_40795

SLMS:

MM221_07870

DOM:	RRU94_10040

AHAL:

FTX54_010725

SHV:	AAT16_04835 AAT16_09650

ESI:

Exig_1251

EAN:

Eab7_1202

EXM:	U719_06490

EXU:	ESP131_08900

BBE:	BBR47_20780 BBR47_22370

BFM:	BP422_05500 BP422_06405

BAGR:

BA6348_22325

BRW:	GOP56_11550

BCHS:

JNE38_11680

BHUI:

LOK74_05870

PJD:	Pjdr2_0135

GYM:	GYMC10_0122

PPM:	PPSC2_00675(epr)

PPO:	PPM_0127(epr)

PPQ:	PPSQR21_001320(aprE)

PPOY:

RE92_11030

PMS:	KNP414_07652

PMQ:	PM3016_7185

PMW:

B2K_36240

PSAB:

PSAB_00510

PDU:	PDUR_00585

PGM:	PGRAT_00580

POD:	PODO_00595

PAEF:

R50345_00610

PSTE:

PSTEL_00945

PAEA:

R70723_00565

PAEE:

R70331_00580

PAEH:

H70357_00585

PAEJ:

H70737_00655

PRI:	PRIO_0115 PRIO_6716(aprE)

PSON:

JI735_08260

PPEO:

ABE82_00685

PNP:	IJ22_44650

POW:	IJ21_02130

PXL:	BS614_03610

PYG:	AWM70_18485

PSWU:

SY83_18965

PDH:	B9T62_32665

PKB:	B4V02_24585

PVO:	PVOR_02741

PLW:	D5F53_29015

PLEN:

EIM92_04075

PPSC:

EHS13_14370 EHS13_32420

PLUT:

EI981_00565

PCHI:

PC41400_29430

PBK:	Back11_45130

PBAC:

HUB98_17660

PRZ:	GZH47_14975

PLYC:

GXP70_02000

PSOP:

KP014_03810

PTRI:

KDC22_00575

PTJ:	JRJ22_00580

PUI:	PUW25_12650

TCO:	Theco_3545

ASOC:

CB4_03352(aprE)

COH:	EAV92_20340

COHN:

KCTCHS21_04720

AFX:	JZ786_07280

SIV:

SSIL_3520

SSIL:

SOLI23_00835

PLN:	Plano_0709

PRT:	AUC31_05580

PLL:	I858_003580

PANA:

BBH88_16715

PDG:	BCM40_12090

PHC:	BBI08_09060 BBI08_16695

PMAR:

B0X71_14850

PPLA:

BBI15_00140

PLX:	CW734_05890

PMAT:

BBI11_05365

PDEC:

H1Q58_04510

PGQ:	FK545_12565

JEO:	JMA_06320 JMA_09790

SURE:

SporoP32a_12710

SPOO:

J3U78_14185

PANC:

E2636_12390

PLAY:

DNR44_007855 DNR44_016300

NTR:	B0W44_11455 B0W44_14375 B0W44_14495

LFB:	C1X05_12925

TVU:	AB849_007900

KPUL:

GXN76_12330

KEB:	GXN75_13860

PABS:

JIR001_24090

CTH:

Cthe_3137

STH:

STH1033

DCA:	Desca_0077

DFG:	B0537_01205

DKU:	Desku_1439

HMO:

HM1_2600

CTHM:

CFE_0732 CFE_0735

ANR:	Ana3638_00755

EAC:	EAL2_808p03370(epr)

MTHO:

MOTHE_c20730(aprE)

MTHZ:

MOTHA_c21500(aprE)

TOC:

Toce_0485

NTH:	Nther_2143

AAR:	Acear_1993

KPAR:

JL105_08405

LPIL:

LIP_0524

TPR:

Tpau_1297

STIR:

DDW44_06165

ALAV:

MTO99_00660

LYD:	D7I47_12140

ARL:	AFL94_13945

ARU:	ASPU41_03075

ACRY:

AC20117_15780

ARTP:

E5206_05210

ASUF:

MNQ99_07240

AAI:	AARI_27140

GCR:	GcLGCM259_2901

GLU:	F0M17_13330

ICA:	Intca_0489

NOY:	EXE57_14510

NCG:	KGD84_24720

TCU:

Tcur_1456

NCX:	Nocox_04630(apr2)

NFS:	OIE67_41885

SEN:

SACE_3537

SACG:

FDZ84_30800

PSEA:

WY02_07925

PSEQ:

AD006_24635

PECQ:

AD017_04225

PHH:	AFB00_21910

PAUT:

Pdca_21980

KPHY:

AOZ06_30845

ALO:

CRK57421

SACE:

GIY23_04895

ASE:	ACPL_4119(aprA)

ACTS:

ACWT_3990

AIH:	Aiant_24880

PBAJ:

LRS13_05360

LET:	O77CONTIG1_01619

LBO:	LBWT_56880

THEU:

HPC62_19325

GDU:	P0S91_08980

MIQ:	B5D77_17445

CYT:

cce_0328

NOE:	CLI64_27730

NAZ:

Aazo_1099

ANB:	ANA_C13330

ACY:	Anacy_0038

AWA:	AA650_04050

CSG:	Cylst_5288

RSIN:

B6N60_03979

CALO:

Cal7507_1584

CALT:

Cal6303_2875

CALH:

IJ00_03035 IJ00_14910

FIS:	FIS3754_31010 FIS3754_47610

DOU:	BMF77_00947(apr)

TOQ:	HCG51_12215

NCN:	BZZ01_26765

RCA:

Rcas_2539

TRO:

trd_1703

DDR:	Deide_3p00270

DMR:	Deima_0142

DGO:	DGo_PA0262

DFC:

DFI_19670

DPH:	EHF33_20920

TSC:	TSC_c03630(apr1)

THC:	TCCBUS3UF1_1430

TOS:	Theos_0005 Theos_1000

TAQ:

TO73_2195

TBC:	A0O31_01516

TANT:

KNN15_06050

MRB:	Mrub_0795 Mrub_1052

MRE:	K649_03615 K649_04905

MTAI:

Mtai_v1c28240

MSV:	Mesil_3207 Mesil_3440

WCH:	wcw_1929(aprE)

ROO:	G5S37_11130

LUO:	HHL09_13970

AMUC:

Pan181_03070

PEH:	Spb1_14850

PLS:	VT03_06235(aprN)

GES:	VT84_14990(apr_1) VT84_17225(aprN)

GOG:	C1280_02115 C1280_14680

GMS:	SOIL9_39940

TSPH:

KIH39_11465

UAM:	UABAM_01285 UABAM_04454

GEX:	GETHOR_01810

FSU:	Fisuc_0830

FSC:

FSU_1274

PCRE:

NCTC12858_00388

PCAG:

NCTC12856_01031

PMUC:

ING2E5A_2610(aprN)

PET:	PEIBARAKI_4625

PSAC:

PSM36_1910

PIT:	PIN17_0039 PIN17_0041 PIN17_0436

MRUB:

DEO27_008250

SLI:	Slin_1710 Slin_3456

SMON:

AWR27_05265

SPIK:

EXU85_27730

STAE:

HNV11_15330 HNV11_19005

SOQ:	LQ777_09535

SFOL:

H3H32_09415

RUN:	DR864_14560

RUP:	DTQ70_06725 DTQ70_23935

FAE:	FAES_2745 FAES_3048(aprE)

FIB:	A6C57_18545 A6C57_23130

HQI:	H9L05_21120

HTB:	MTX78_08195

PRHO:

PZB74_14670

FCO:	FCOL_07585

CLK:	CGC53_00640

CNP:	M0D58_14185

CJG:	NCTC13459_00605(apr)

FTE:	Fluta_2004

OHO:	Oweho_0297

PAA:

Paes_2280

PHY:	AJ81_02930

MPG:	Theba_1167

MINF:

MESINF_2231

AVE:	Arcve_0834 Arcve_0894

FPL:

Ferp_2094

THS:

TES1_1967

THV:	ADU37_CDS05690

TGG:	A3K92_06795

PPAC:

PAP_01675

MBAK:

MSBR3_1511

MTHR:

MSTHT_0610

MTHE:

MSTHC_0110

MHOR:

MSHOH_1113

MFZ:	AOB57_013815

MPY:

Mpsy_0077

MZI:	HWN40_07195

MMAV:

RE476_03310

MSEB:

RE474_12975

MHZ:	Metho_2263

MPD:

MCP_1283

HALX:

M0R89_16480 M0R89_16485 M0R89_16490 M0R89_16495

HAXZ:

M0R88_09685 M0R88_17075 M0R88_17085 M0R88_17090

HAYC:

NGM10_06340 NGM10_14420 NGM10_14430 NGM10_14435 NGM10_14440

HAXY:

NGM07_02950 NGM07_02955 NGM07_16990 NGM07_24425

HARA:

AArcS_0205(aprE)

NSAL:

HWV07_05120 HWV07_09995

HAAD:

MW046_00465 MW046_00730 MW046_00885

HME:	HFX_5138(aprE) HFX_5181(aprE)

HBO:	Hbor_32310 Hbor_34040

HAQ:	DU484_07355

HAJ:	DU500_07470

HALM:

FCF25_13890 FCF25_13895

HLA:

Hlac_2196

HALN:

B4589_002575

HALG:

HUG10_06465

HALU:

HUG12_10560

HRE:	K6T36_05120

HACB:

Hbl1158_11245

HDF:	AArcSl_0415(aprE)

HAH:	Halar_2141 Halar_2573

HTU:

Htur_0058

HSAL:

JMJ58_09075

HAKZ:

J0X25_06810

NMG:

Nmag_1874

HXA:	Halxa_1240

NPE:	Natpe_0106

NVR:	FEJ81_00555

NAY:	HYG81_10125

HJT:	DVR14_09920

NGE:	Natgr_3003 Natgr_3703

HRU:	Halru_0481 Halru_0482

SAWL:

NGM29_09345

HALV:

NGM15_01570

NOU:	Natoc_2188

HLR:	HALLA_03780

HLC:	CHINAEXTREME11860

HDA:	BB347_14195

PFM:	Pyrfu_1125

PDL:	Pyrde_0048

THB:	N186_08410

TCB:	TCARB_0761

THF:	MA03_03015

NIW:	Nisw_02775

TAA:	NMY3_00666 NMY3_03174

NFN:	NFRAN_0204

NIU:	DSQ19_01525 DSQ19_03065

NCK:	QVH35_11630

» show all

Reference

Authors

Ottesen, M. and Svendsen, I.

Title

The subtilisins.

Journal

Methods Enzymol 19:199-215 (1970)

Reference

Authors

Markland, F.S. and Smith, E.L.

Title

Subtilisins: primary structure, chemical and physical properties.

Journal

In: Boyer, P.D. (Ed.), The Enzymes, 3rd ed., vol. 3, Academic Press, New York, 1971, p. 561-608.

Reference

3 [PMID:6221910]

Authors

Tang H.

Title

[The use of single-dose total body irradiation before bone marrow transplantation in treatment of leukemia]

Journal

Zhonghua Fang She Xue Za Zhi 16:296-300 (1982)

Reference

4 [PMID:3927935]

Authors

Nedkov P, Oberthur W, Braunitzer G.

Title

Determination of the complete amino-acid sequence of subtilisin DY and its comparison with the primary structures of the subtilisins BPN', Carlsberg and amylosacchariticus.

Journal

Biol Chem Hoppe Seyler 366:421-30 (1985)
DOI:10.1515/bchm3.1985.366.1.421

Reference

5 [PMID:3108260]

Authors

Ikemura H, Takagi H, Inouye M

Title

Requirement of pro-sequence for the production of active subtilisin E in Escherichia coli.

Journal

J Biol Chem 262:7859-64 (1987)

Sequence

[bsu:BSU10300]