KEGG   ENZYME: 3.4.21.99
Entry
EC 3.4.21.99                Enzyme                                 
Name
spermosin
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases
Reaction(IUBMB)
Hydrolyses arginyl bonds, preferably with Pro in the P2 position
Comment
The enzyme from the ascidian (Prochordate) Halocynthia roretzi is localized in the sperm head, and released during sperm activation. A proline-rich region is involved in binding to the vitelline coat of the egg. Belongs in peptidase family S1 (trypsin family).
History
EC 3.4.21.99 created 2001
Orthology
K20751  spermosin
Genes
CIN: 100185187
SCLV: 120342178
Reference
1  [PMID:6365918]
  Authors
Sawada H, Yokosawa H, Ishii S.
  Title
Purification and characterization of two types of trypsin-like enzymes from sperm of the ascidian (Prochordata) Halocynthia roretzi. Evidence for the presence of spermosin, a novel acrosin-like enzyme.
  Journal
J Biol Chem 259:2900-4 (1984)
  Sequence
[ag:BAB60896]
Reference
2  [PMID:6381175]
  Authors
Sawada H, Yokosawa H, Someno T, Saino T, Ishii S.
  Title
Evidence for the participation of two sperm proteases, spermosin and acrosin, in fertilization of the ascidian, Halocynthia roretzi: inhibitory effects of leupeptin analogs on enzyme activities and fertilization.
  Journal
Dev Biol 105:246-9 (1984)
DOI:10.1016/0012-1606(84)90281-1
  Sequence
[ag:BAB60896]
Reference
3  [PMID:8670234]
  Authors
Sawada H, Iwasaki K, Kihara-Negishi F, Ariga H, Yokosawa H.
  Title
Localization, expression, and the role in fertilization of spermosin, an ascidian sperm trypsin-like protease.
  Journal
Biochem Biophys Res Commun 222:499-504 (1996)
DOI:10.1006/bbrc.1996.0773
  Sequence
[ag:BAB60896]
Reference
4  [PMID:8914083]
  Authors
Sawada H, Someno T
  Title
Substrate specificity of ascidian sperm trypsin-like proteases, spermosin and acrosin.
  Journal
Mol Reprod Dev 45:240-3 (1996)
DOI:10.1002/(SICI)1098-2795(199610)45:2<240::AID-MRD18>3.0.CO;2-4
  Sequence
[ag:BAB60896]
Other DBs
ExplorEnz - The Enzyme Database: 3.4.21.99
IUBMB Enzyme Nomenclature: 3.4.21.99
ExPASy - ENZYME nomenclature database: 3.4.21.99
BRENDA, the Enzyme Database: 3.4.21.99
CAS: 89925-67-7

  All links  
Gene (4)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (3)   
Protein sequence (2)   
   UniProt (1)   
   SWISS-PROT (1)   
Protein domain (7)   
   InterPro (7)   
All databases (13)   

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