KEGG   ENZYME: 3.4.22.25
Entry
EC 3.4.22.25                Enzyme                                 
Name
glycyl endopeptidase;
papaya peptidase B;
papaya proteinase IV;
glycine-specific proteinase;
chymopapain;
Papaya proteinase 4;
PPIV;
chymopapain M
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Cysteine endopeptidases
Reaction(IUBMB)
Preferential cleavage: Gly!, in proteins and small molecule substrates
Comment
From the papaya plant, Carica papaya. Not inhibited by chicken cystatin, unlike most other homologues of papain, but in peptidase family C1 (papain family)
History
EC 3.4.22.25 created 1992
Orthology
K20740  glycyl endopeptidase
Genes
CPAP: 110807982
Reference
1  [PMID:7018581]
  Authors
Polgar L.
  Title
Isolation of highly active papaya peptidases A and B from commercial chymopapain.
  Journal
Biochim Biophys Acta 658:262-9 (1981)
DOI:10.1016/0005-2744(81)90296-5
Reference
2  [PMID:2505761]
  Authors
Buttle DJ, Kembhavi AA, Sharp SL, Shute RE, Rich DH, Barrett AJ.
  Title
Affinity purification of the novel cysteine proteinase papaya proteinase IV, and papain from papaya latex.
  Journal
Biochem J 261:469-76 (1989)
DOI:10.1042/bj2610469
Reference
3  [PMID:2591528]
  Authors
Ritonja A, Buttle DJ, Rawlings ND, Turk V, Barrett AJ
  Title
Papaya proteinase IV amino acid sequence.
  Journal
FEBS Lett 258:109-12 (1989)
DOI:10.1016/0014-5793(89)81627-8
  Sequence
[ag:CAA54974]
Reference
4  [PMID:2404797]
  Authors
Buttle DJ, Ritonja A, Pearl LH, Turk V, Barrett AJ
  Title
Selective cleavage of glycyl bonds by papaya proteinase IV.
  Journal
FEBS Lett 260:195-7 (1990)
DOI:10.1016/0014-5793(90)80101-N
  Sequence
[ag:CAA54974]
Reference
5  [PMID:1690669]
  Authors
Buttle DJ, Ritonja A, Dando PM, Abrahamson M, Shaw EN, Wikstrom P, Turk V, Barrett AJ.
  Title
Interactions of papaya proteinase IV with inhibitors.
  Journal
FEBS Lett 262:58-60 (1990)
DOI:10.1016/0014-5793(90)80153-A
Other DBs
ExplorEnz - The Enzyme Database: 3.4.22.25
IUBMB Enzyme Nomenclature: 3.4.22.25
ExPASy - ENZYME nomenclature database: 3.4.22.25
BRENDA, the Enzyme Database: 3.4.22.25
CAS: 149719-24-4

  All links  
Gene (3)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (2)   
Protein sequence (9)   
   UniProt (4)   
   SWISS-PROT (1)   
   RefSeq(pep) (2)   
   PDBSTR (2)   
DNA sequence (3)   
   RefSeq(nuc) (3)   
3D Structure (1)   
   PDB (1)   
Protein domain (9)   
   InterPro (8)   
   PROSITE(DOC) (1)   
All databases (25)   

Download RDF
DBGET integrated database retrieval system