KEGG   ENZYME: 3.4.22.33
Entry
EC 3.4.22.33                Enzyme                                 
Name
fruit bromelain;
juice bromelain;
ananase;
bromelase;
bromelin;
extranase;
pinase;
pineapple enzyme;
traumanase;
fruit bromelain FA2
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Cysteine endopeptidases
Reaction(IUBMB)
Hydrolysis of proteins with broad specificity for peptide bonds. Bz-Phe-Val-Arg!NHMec is a good synthetic substrate, but there is no action on Z-Arg-Arg-NHMec (c.f. stem bromelain)
Comment
From the pineapple plant, Ananas comosus. Scarcely inhibited by chicken cystatin. Another cysteine endopeptidase, with similar action on small molecule substrates, pinguinain, is obtained from the related plant, Bromelia pinguin, but pinguinain differs from fruit bromelain in being inhibited by chicken cystatin [4].
History
EC 3.4.22.33 created 1965 as EC 3.4.4.24, transferred 1972 to EC 3.4.22.4, part transferred 1992 to EC 3.4.22.33
Reference
1  [PMID:4127920]
  Authors
Sasaki M, Kato T, Iida S.
  Title
Antigenic determinant common to four kinds of thiol proteases of plant origin.
  Journal
J Biochem (Tokyo) 74:635-7 (1973)
DOI:10.1093/oxfordjournals.jbchem.a130287
Reference
2  [PMID:956152]
  Authors
Yamada F, Takahashi N, Murachi T.
  Title
Purification and characterization of a proteinase from pineapple fruit, fruit bromelain FA2.
  Journal
J Biochem (Tokyo) 79:1223-34 (1976)
DOI:10.1093/oxfordjournals.jbchem.a131176
Reference
3  [PMID:4044551]
  Authors
Ota S, Muta E, Katahira Y, Okamoto Y.
  Title
Reinvestigation of fractionation and some properties of the proteolytically active components of stem and fruit bromelains.
  Journal
J Biochem (Tokyo) 98:219-28 (1985)
DOI:10.1093/oxfordjournals.jbchem.a135261
Reference
4  [PMID:2327970]
  Authors
Rowan AD, Buttle DJ, Barrett AJ.
  Title
The cysteine proteinases of the pineapple plant.
  Journal
Biochem J 266:869-75 (1990)
Other DBs
ExplorEnz - The Enzyme Database: 3.4.22.33
IUBMB Enzyme Nomenclature: 3.4.22.33
ExPASy - ENZYME nomenclature database: 3.4.22.33
BRENDA, the Enzyme Database: 3.4.22.33
CAS: 9001-00-7

  All links  
Protein sequence (162)   
   UniProt (150)   
   SWISS-PROT (1)   
   RefSeq(pep) (11)   
DNA sequence (47)   
   RefSeq(nuc) (19)   
   GenBank (15)   
   EMBL (13)   
Protein domain (8)   
   InterPro (8)   
All databases (217)   

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