KEGG ENZYME: 3.4.22.54

ENZYME: 3.4.22.54

Entry

EC 3.4.22.54                Enzyme

Name

calpain-3;
p94;
calpain p94;
CAPN3;
muscle calpain;
calpain 3;
calcium-activated neutral proteinase 3;
muscle-specific calcium-activated neutral protease 3;
CANP 3;
calpain L3

Class

Hydrolases;
Acting on peptide bonds (peptidases);
Cysteine endopeptidases

Reaction(IUBMB)

Broad endopeptidase activity

Comment

This Ca2+-dependent enzyme is found in skeletal muscle and is genetically linked to limb girdle muscular dystrophy type 2A [1,4]. The enzyme is activated by autoproteolytic cleavage of insertion sequence 1 (IS1), which allows substrates and inhibitors gain access to the active site [4]. Substrates include the protein itself [3,4] and connectin/titin [2,5]. Belongs in peptidase family C2.

History

EC 3.4.22.54 created 2007

Orthology

K08573

calpain-3

Genes

HSA:	825(CAPN3)

PPS:

100984344

PON:	100449804(CAPN3)

HMH:	116482515(CAPN3)

MCC:	707913(CAPN3)

MCF:	102115794(CAPN3)

MTHB:

126959809

MNI:	105491693(CAPN3)

CSAB:

103245772(CAPN3)

CATY:

105574217(CAPN3)

PANU:

101003739(CAPN3)

TGE:	112627441(CAPN3)

MLEU:

105546143(CAPN3)

RRO:	104665262(CAPN3)

RBB:	108521152(CAPN3)

TFN:	117070804(CAPN3)

PTEH:

111529157

CANG:

105511067(CAPN3)

CJC:	100389438(CAPN3)

SBQ:	101035050(CAPN3)

CIMI:

108294422(CAPN3)

CSYR:

103261776(CAPN3)

MMUR:

105872501(CAPN3)

LCAT:

123630188(CAPN3)

PCOQ:

105814391(CAPN3)

OGA:	100964370(CAPN3)

MMU:	12335(Capn3)

MCAL:

110309710(Capn3)

MPAH:

110318292(Capn3)

RNO:	29155(Capn3)

MCOC:

116090761(Capn3)

ANU:	117702858(Capn3)

MUN:	110549916(Capn3)

CGE:	100758723(Capn3)

MAUA:

101822522(Capn3)

PROB:

127221592(Capn3)

PLEU:

114681123(Capn3)

MORG:

121465595(Capn3)

MFOT:

126494432

AAMP:

119814387(Capn3)

NGI:	103744953(Capn3)

HGL:	101707543(Capn3)

CPOC:

100735679(Capn3)

CCAN:

109690734(Capn3)

DORD:

105981170(Capn3)

DSP:	122114613(Capn3)

PLOP:

125340953(Capn3)

NCAR:

124977436

MMMA:

107138183(Capn3)

OCU:	100008726(CAPN3)

OPI:	101533222(CAPN3)

TUP:	102468105(CAPN3)

GVR:	103600898(CAPN3)

CFA:	100856545(CAPN3)

CLUD:

112676067(CAPN3)

VVP:	112919983(CAPN3)

VLG:	121485770(CAPN3)

NPO:	129504162(CAPN3)

AML:	100478387(CAPN3)

UMR:	103673424(CAPN3)

UAH:	113241990(CAPN3)

UAR:	123775845(CAPN3)

ELK:

111160918

LLV:

125105494

MPUF:

101675191(CAPN3)

MLK:	131836166(CAPN3)

NVS:	122892893(CAPN3)

ORO:	101384331(CAPN3)

EJU:	114201673(CAPN3)

ZCA:	113910306(CAPN3)

MLX:	118003460(CAPN3)

NSU:	110585421(CAPN3)

LWW:	102746966(CAPN3)

FCA:	101081304(CAPN3)

PYU:	121041570(CAPN3)

PCOO:

112856247(CAPN3)

PBG:	122467655(CAPN3)

PVIV:

125167938(CAPN3)

LRUF:

124508078

PTG:	102962051(CAPN3)

PPAD:

109263103(CAPN3)

PUC:

125909904

AJU:

106968505

HHV:	120232213(CAPN3)

BTA:	281663(CAPN3)

BOM:	102284625(CAPN3)

BIU:	109564987(CAPN3)

BBUB:

102393431(CAPN3)

BBIS:

104994443(CAPN3)

CHX:	102189762(CAPN3)

OAS:	443038(CAPN3)

BTAX:

128053913(CAPN3)

ODA:	120853162(CAPN3)

CCAD:

122443440(CAPN3)

MBEZ:

129562575(CAPN3)

SSC:	397349(CAPN3)

CFR:	102518250(CAPN3)

CBAI:

105076938(CAPN3)

CDK:	105101740(CAPN3)

VPC:	102530952(CAPN3)

LVE:	103088026(CAPN3)

OOR:	101289409(CAPN3)

DLE:	111181696(CAPN3)

PCAD:

102985687(CAPN3)

PSIU:

116749382(CAPN3)

NASI:

112391183(CAPN3)

ECB:	100056798(CAPN3)

EPZ:	103549943(CAPN3)

EAI:	106841338(CAPN3)

MYB:	102245181(CAPN3)

MYD:	102768421(CAPN3)

MMYO:

118650353(CAPN3)

MLF:	102423101(CAPN3)

PKL:	118722897(CAPN3)

EFUS:

103287348(CAPN3)

MNA:	107536546(CAPN3)

DRO:	112311921(CAPN3)

AJM:	119061279(CAPN3)

PDIC:

114492273(CAPN3)

PHAS:

123808805(CAPN3)

MMF:	118641423(CAPN3)

PPAM:

129064324(CAPN3)

RFQ:	117024234(CAPN3)

PALE:

102886269(CAPN3)

PGIG:

120622749(CAPN3)

PVP:	105288790(CAPN3)

RAY:	107510930(CAPN3)

MJV:	108386637(CAPN3)

TOD:	119238530(CAPN3)

SARA:

101558331(CAPN3)

LAV:	100667912(CAPN3)

TMU:

101356561

ETF:	101638394(CAPN3)

DNM:	101428782(CAPN3)

MDO:	100010062(CAPN3)

GAS:	123237897(CAPN3)

SHR:	100916328(CAPN3)

AFZ:

127549283

PCW:	110223679(CAPN3)

OAA:	100084116(CAPN3)

GGA:	423233(CAPN3)

MGP:	100546035(CAPN3)

CJO:	107314753(CAPN3)

TPAI:

128085680(CAPN3)

LMUT:

125694509(CAPN3)

NMEL:

110401942(CAPN3)

APLA:

101792647(CAPN3)

ACYG:

106042918(CAPN3)

CATA:

118244573(CAPN3)

AFUL:

116489626(CAPN3)

TGU:	100225837(CAPN3)

LSR:	110472120(CAPN3)

SCAN:

103826645(CAPN3)

PMOA:

120500534(CAPN3)

OTC:	121335792(CAPN3)

PRUF:

121349554(CAPN3)

GFR:	102042342(CAPN3)

FAB:	101814350(CAPN3)

OMA:	130254444(CAPN3)

PHI:	102107172(CAPN3)

PMAJ:

107205589(CAPN3)

CCAE:

111930233(CAPN3)

CCW:	104698659(CAPN3)

CBRC:

103620775(CAPN3)

ETL:	114056480(CAPN3)

ZAB:	102071927(CAPN3)

ACHL:

103801729(CAPN3)

SVG:	106848997(CAPN3)

MMEA:

130573546(CAPN3)

HRT:	120753965(CAPN3)

FPG:	101924532(CAPN3)

FCH:	102055944(CAPN3)

CLV:	102094715(CAPN3)

EGZ:	104127554(CAPN3)

NNI:	104020344(CAPN3)

PCRI:

104024917(CAPN3)

PLET:

104625460(CAPN3)

EHS:	104515389(CAPN3)

PCAO:

104043793(CAPN3)

ACUN:

113480542(CAPN3)

TALA:

104359490(CAPN3)

PADL:

103912804(CAPN3)

AFOR:

103897520(CAPN3)

ACHC:

115349507(CAPN3)

HALD:

104325215(CAPN3)

HLE:	104839649(CAPN3)

AGEN:

126049193

GCL:

127016432

CCRI:

104162432(CAPN3)

CSTI:

104555939

CMAC:

104474015(CAPN3)

BREG:

104638113(CAPN3)

FGA:

104080460

GSTE:

104263026(CAPN3)

LDI:	104340731(CAPN3)

MNB:

103775218

OHA:	104328119(CAPN3)

NNT:	104410103(CAPN3)

SHAB:

115606495(CAPN3)

DPUB:

104298852(CAPN3)

PGUU:

104458993(CAPN3)

ACAR:

104532886(CAPN3)

CPEA:

104388443(CAPN3)

AVIT:

104273019(CAPN3)

CVF:	104289069(CAPN3)

RTD:	128908873(CAPN3)

CUCA:

104067323(CAPN3)

TEO:	104380706(CAPN3)

BRHI:

104492893(CAPN3)

AROW:

112964040(CAPN3)

NPD:	112942521(CAPN3)

DNE:	112980626(CAPN3)

SCAM:

104150406(CAPN3)

ASN:	102379878(CAPN3)

AMJ:	102577345(CAPN3)

CPOO:

109321300(CAPN3)

GGN:	109298795(CAPN3)

PSS:	102450185(CAPN3)

CMY:	102936637(CAPN3)

CCAY:

125638798(CAPN3)

DCC:	119856903(CAPN3)

CPIC:

101952809(CAPN3)

TST:	117876784(CAPN3)

CABI:

116825465(CAPN3)

MRV:	120403813(CAPN3)

ACS:	100559629(capn3)

ASAO:

132766589(CAPN3)

PVT:	110079031(CAPN3)

SUND:

121919140(CAPN3)

PBI:	103066997(CAPN3)

PMUR:

107295757(CAPN3)

CTIG:

120300708(CAPN3)

TSR:	106547747(CAPN3)

PGUT:

117665635(CAPN3)

APRI:

131187587(CAPN3)

PTEX:

113442185(CAPN3)

NSS:	113414257(CAPN3)

VKO:	123026806(CAPN3)

PMUA:

114594341(CAPN3)

PRAF:

128411373(CAPN3)

ZVI:	118083644(CAPN3)

HCG:	128340139(CAPN3)

GJA:	107117895(CAPN3)

STOW:

125427212(CAPN3)

EMC:	129324117(CAPN3)

XLA:	100125665(capn3.L) 108700461

XTR:	100497532(capn3)

NPR:	108797818(CAPN3)

RTEM:

120920795(CAPN3)

BBUF:

120981853(CAPN3)

BGAR:

122921245(CAPN3)

MUO:	115478040(CAPN3)

GSH:	117364048(CAPN3)

DRE:	447832(capn3a)

SRX:	107732302 107739229

SANH:

107664429(capn3)

SGH:

107586853

CAUA:

113060520 113117063

CGIB:

127976709

PTET:

122362108(capn3a)

LROH:

127180254(capn3a)

OMC:	131522870(capn3a)

PPRM:

120464073(capn3a)

RKG:	130080204(capn3a)

MAMB:

125269025(capn3a)

CIDE:

127498978

MASI:

127411246 127454769

TROS:

130560049(capn3a)

TDW:	130436767(capn3a)

MANU:

129418082(capn3a)

IPU:	108269768(capn3a)

IFU:	128613021(capn3a)

PHYP:

113538734

SMEO:

124390085(capn3a)

TFD:	113659539(capn3a)

TVC:	132852566(capn3a)

CMAO:

118823930(capn3a)

EEE:

113587020

CHAR:

105899114(capn3a)

TRU:	101075691 101077986(capn3)

TFS:	130513329(capn3a) 130515702(capn3b)

TNG:	GSTEN00019931G001

LCO:

104936553

NCC:	104945865(capn3)

TBEN:

117486936(capn3b) 117493771(capn3a)

CGOB:

115027228(capn3) 115028992

PGEO:

117439943(capn3b) 117467960(capn3a)

GACU:

117542903 117549038(capn3a) 117555691(capn3b)

EMAC:

134876635(capn3b) 134881072(capn3a)

ELY:	117267460(capn3a) 117270774(capn3b)

EFO:	125897620(capn3b) 125901103(capn3a)

PLEP:

121953730(capn3a) 121955200(capn3b)

SLUC:

116057697(capn3a) 116066425(capn3b)

ECRA:

117936309(capn3a) 117961699(capn3b)

ESP:	116686083(capn3) 116705817

PFLV:

114546330(capn3) 114573031

GAT:	120808321(capn3b) 120833187(capn3a)

PPUG:

119194637(capn3b) 119227420(capn3a)

AFB:	129102973(capn3a) 129107456(capn3b)

CLUM:

117727050(capn3b) 117751379(capn3a)

PSWI:

130201752(capn3a) 130202117(capn3b)

MSAM:

119909041(capn3b) 119909908(capn3a)

SCHU:

122861776(capn3a) 122863461(capn3b)

CUD:	121521109(capn3b) 121526230(capn3a)

ALAT:

119005030(capn3a) 119012575(capn3b)

MZE:	101463601 101466182(capn3)

ONL:	100691885(capn3) 100699569

OAU:	116318066(capn3a) 116324143(capn3b)

OLA:	101157263 101169329

OML:	112144429(capn3a) 112158058(capn3b)

CSAI:

133418611(capn3b) 133462927(capn3a)

XMA:	102221680(capn3) 102222528

XCO:	114134598(capn3) 114158499

XHE:	116708429(capn3) 116733763

PRET:

103457431 103458212(capn3)

PFOR:

103133981 103150049(capn3)

PLAI:

106952040 106953715

PMEI:

106919034(capn3) 106924031

GAF:	122825756(capn3b) 122846114(capn3a)

PPRL:

129375248(capn3b) 129377228(capn3a)

CVG:	107093158 107096801(capn3)

CTUL:

119779718(capn3a)

GMU:	124856039(capn3a) 124882084(capn3b)

NFU:	107377674 107391866(capn3)

KMR:	108233461(capn3b) 108234555(capn3a)

ALIM:

106524302(capn3) 106537237

NWH:	119418575(capn3b) 119426946(capn3a)

AOCE:

111574533 111587838

MCEP:

124998693(capn3b) 125023329(capn3a)

CSEM:

103380923 103381555

POV:	109636052 109644862

SSEN:

122783338(capn3a) 122784464(capn3b)

HHIP:

117755906(capn3a) 117757990(capn3b)

HSP:	118099302(capn3b) 118117058(capn3a)

SMAU:

118285806(capn3a) 118290255(capn3b)

LCF:	108876634(capn3a) 108895964

SDU:	111225368(capn3) 111237184

SLAL:

111646798(capn3) 111661373

XGL:	120788896(capn3b) 120795487(capn3a)

HCQ:	109508050(capn8) 109517476(capn3)

SSCV:

125981134 125990290

SBIA:

133497039(capn3b) 133513735(capn3a)

PEE:	133412495(capn3a) 133416781(capn3b)

PTAO:

133468094(capn3b) 133487602(capn3a)

BPEC:

110157545(capn3) 110173943

MALB:

109953348 109962461(capn3)

BSPL:

114849042(capn3a) 114849713(capn3b)

SASA:

100306869(can3) 106568467(capn3) 106611143

STRU:

115161830 115173034 115175025

OTW:	112241717 112249945(capn3b) 112255824(capn3a)

OMY:	110498417 110505915(capn3a) 110529398(capn3b)

OGO:	123990572(capn3b) 124002235 124046822(capn3a)

ONE:	115107570 115138850

OKI:	109880971 109883062 109900562

OKE:

118380901

SALP:

111954339(capn3)

SNH:	120024248(capn3b) 120035919(capn3a)

CCLU:

121539129 121544526(capn3a) 121552620(capn3b)

ELS:	105015344 105015896(capn3)

SFM:

108924080

PKI:	111859952(capn3)

AANG:

118223800(capn3a) 118229185

LOC:	102695785(capn3)

PSPA:

121324768

ARUT:

117422311(capn3a)

PSEX:

120518426(capn3a)

LCM:	102358118(CAPN3)

CMK:

103190049

RTP:	109927910(capn3a)

CPLA:

122553855(capn3a)

HOC:	132818086 132836295

LERI:

129714207

PMRN:

116939632 116943170 116947735 116947736

LRJ:	133341426 133341428 133342411 133347253 133352610

BFO:

118430052

BBEL:

109472173

CIN:

100185355

SCLV:

120327282

NLU:

120353930

HAME:

121860621

ESN:

126987559

EAF:

111705561

PVUL:

126831628

BGT:

106078254

» show all

Reference

1 [PMID:2555341]

Authors

Sorimachi H, Imajoh-Ohmi S, Emori Y, Kawasaki H, Ohno S, Minami Y, Suzuki K.

Title

Molecular cloning of a novel mammalian calcium-dependent protease distinct from both m- and mu-types. Specific expression of the mRNA in skeletal muscle.

Journal

J Biol Chem 264:20106-11 (1989)

Sequence

[hsa:825] [rno:29155]

Reference

2 [PMID:8537379]

Authors

Sorimachi H, Kinbara K, Kimura S, Takahashi M, Ishiura S, Sasagawa N, Sorimachi N, Shimada H, Tagawa K, Maruyama K, et al.

Title

Muscle-specific calpain, p94, responsible for limb girdle muscular dystrophy type 2A, associates with connectin through IS2, a p94-specific sequence.

Journal

J Biol Chem 270:31158-62 (1995)
DOI:10.1074/jbc.270.52.31158

Reference

3 [PMID:12482600]

Authors

Rey MA, Davies PL.

Title

The protease core of the muscle-specific calpain, p94, undergoes Ca2+-dependent intramolecular autolysis.

Journal

FEBS Lett 532:401-6 (2002)
DOI:10.1016/S0014-5793(02)03722-5

Reference

4 [PMID:16533054]

Authors

Garcia Diaz BE, Gauthier S, Davies PL.

Title

Ca2+ dependency of calpain 3 (p94) activation.

Journal

Biochemistry 45:3714-22 (2006)
DOI:10.1021/bi051917j

Reference

5 [PMID:16627476]

Authors

Ono Y, Torii F, Ojima K, Doi N, Yoshioka K, Kawabata Y, Labeit D, Labeit S, Suzuki K, Abe K, Maeda T, Sorimachi H.

Title

Suppressed disassembly of autolyzing p94/CAPN3 by N2A connectin/titin in a genetic reporter system.

Journal

J Biol Chem 281:18519-31 (2006)
DOI:10.1074/jbc.M601029200

Other DBs

ExplorEnz - The Enzyme Database:

3.4.22.54

IUBMB Enzyme Nomenclature:

3.4.22.54

ExPASy - ENZYME nomenclature database:

3.4.22.54

BRENDA, the Enzyme Database:

3.4.22.54

DBGET integrated database retrieval system