KEGG   ENZYME: 3.4.23.18
Entry
EC 3.4.23.18                Enzyme                                 
Name
aspergillopepsin I;
Aspergillus acid protease;
Aspergillus acid proteinase;
Aspergillus aspartic proteinase;
Aspergillus awamori acid proteinase;
Aspergillus carboxyl proteinase;
(see also Comments);
carboxyl proteinase;
Aspergillus kawachii aspartic proteinase;
Aspergillus saitoi acid proteinase;
pepsin-type aspartic proteinase;
Aspergillus niger acid proteinase;
sumizyme AP;
proctase P;
denapsin;
denapsin XP 271;
proctase
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Aspartic endopeptidases
Reaction(IUBMB)
Hydrolysis of proteins with broad specificity. Generally favours hydrophobic residues in P1 and P1', but also accepts Lys in P1, which leads to activation of trypsinogen. Does not clot milk
Comment
Found in a variety of Aspergillus species (imperfect fungi): Aspergillus awamori (awamorin, aspergillopepsin A: [8]), A. foetidus (aspergillopepsin F: [6]), A. fumigatus [7], A. kawachii [9], A. niger (proteinase B, proctase B: [2,4]), A. oryzae (trypsinogen kinase: [3,10]), A. saitoi (aspergillopeptidase A: [10]), and A. sojae [5,10]. In peptidase family A1 (pepsin A family). Formerly included in EC 3.4.23.6
History
EC 3.4.23.18 created 1992 (EC 3.4.23.6 created 1961 as EC 3.4.4.17, transferred 1972 to EC 3.4.23.6, modified 1981 [EC 3.4.23.7, EC 3.4.23.8, EC 3.4.23.9, EC 3.4.23.10, EC 3.4.99.1, EC 3.4.99.15 and EC 3.4.99.25 all created 1972 and incorporated 1978], part incorporated 1992)
Orthology
K06004  aspergillopepsin I
Genes
FPOAFPOAC1_005815 FPOAC1_010867
FVNFVRRES_06413 FVRRES_12522
FKRNCS57_01092400 NCS57_01295100
FMUJ7337_007417 J7337_012363 J7337_012944
ANIANIA_06888
AFMAFUA_5G13300
ACTACLA_016280
NFINFIA_073740
AORAO090120000474
AFVAFLA_009680
ALUCAKAW2_41121A(PEP1)
ACHEACHE_51170S(PEP1)
APUUAPUU_12203A(PEP1_1) APUU_51484S(PEP1_2)
POUPOX_d04885 POX_d05452
TMFEYB26_001150 EYB26_001251 EYB26_002359 EYB26_002360 EYB26_002361 EYB26_002364 EYB26_002376 EYB26_002385 EYB26_002392 EYB26_002403 EYB26_002417 EYB26_002713 EYB26_004552 EYB26_004969 EYB26_005057 EYB26_005064 EYB26_005519 EYB26_006326 EYB26_006327 EYB26_006961 EYB26_007507 EYB26_007535 EYB26_007804 EYB26_008179 EYB26_009132 EYB26_010036 EYB26_010041
TRGTRUGW13939_01228 TRUGW13939_01680 TRUGW13939_05450 TRUGW13939_07522
ABEARB_05728
TVETRV_03007
BGHBDBG_04134
ARABEKO05_0007000
FFUCLAFUR5_03107
CBETCB0940_02794
 » show all
Reference
1  [PMID:4565799]
  Authors
Kovaleva GG, Shimanskaya MP, Stepanov VM.
  Title
The site of diazoacetyl inhibitor attachment to acid proteinase of Aspergillus awamori--an analog of penicillopepsin and pepsin.
  Journal
Biochem Biophys Res Commun 49:1075-81 (1972)
DOI:10.1016/0006-291X(72)90322-1
Reference
2  [PMID:4593189]
  Authors
Morihara K, Oka T.
  Title
Comparative specificity of microbial acid proteinases for synthetic peptides. 3. Relationship with their trypsinogen activating ability.
  Journal
Arch Biochem Biophys 157:561-72 (1973)
Reference
3  [PMID:239702]
  Authors
Davidson R, Gertler A, Hofmann T.
  Title
Aspergillus oryzae acid proteinase. Purification and properties, and formation of pi-chymotrypsin.
  Journal
Biochem J 147:45-53 (1975)
DOI:10.1042/bj1470045
Reference
4  [PMID:12156]
  Authors
Chang WJ, Horiuchi S, Takahashi K, Yamasaki M, Yamada Y
  Title
The structure and function of acid proteases. VI. Effects of acid protease-specific inhibitors on the acid proteases from Aspergillus niger var. macrosporus.
  Journal
J Biochem 80:975-81 (1976)
DOI:10.1093/oxfordjournals.jbchem.a131385
Reference
5  [PMID:21699]
  Authors
Tanaka N, Takeuchi M, Ichishima E.
  Title
Purification of an acid proteinase from Aspergillus saitoi and determination of peptide bond specificity.
  Journal
Biochim Biophys Acta 485:406-16 (1977)
DOI:10.1016/0005-2744(77)90176-0
Reference
6
  Authors
Ostoslavskaya, V.I., Kotlova, E.K., Stepanov, V.M., Rudenskaya, G.H., Baratova, L.A. and Belyanova, L.P.
  Title
Aspergillopepsin F-A carboxylic proteinase from Aspergillus foetidus.
  Journal
Bioorg Khim 5:595-603 (1976)
Reference
7  [PMID:7024192]
  Authors
Panneerselvam M, Dhar SC.
  Title
Studies on the peptide bond specificity and the essential groups of an acid proteinase from Aspergillus fumigatus.
  Journal
Ital J Biochem 30:207-16 (1981)
Reference
8
  Authors
Ostoslavskaya, V.I., Revina, L.P., Kotlova, E.K., Surova, I.A., Levin, E.D., Timokhima, E.A. and Stepanov, V.M.
  Title
The primary structure of aspergillopepsin A, aspartic proteinase from Aspergillus awamori. IV. Amino acid sequence of the enzyme.
  Journal
Bioorg Khim 12:1030-1047 (1986)
Reference
9
  Authors
Yagi, F., Fan, J., Tadera, K. and Kobayashi, A.
  Title
Purification and characterization of carboxyl proteinase from Aspergillus kawachii.
  Journal
Agric Biol Chem 50:1029-1033 (1986)
Reference
10
  Authors
Majima, E., Oda, K., Murao, S. and Ichishima, E.
  Title
Comparative study on the specificities of several fungal aspartic and acidic proteinases towards the tetradecapeptide of a renin substrate.
  Journal
Agric Biol Chem 52:787-793 (1988)
Other DBs
ExplorEnz - The Enzyme Database: 3.4.23.18
IUBMB Enzyme Nomenclature: 3.4.23.18
ExPASy - ENZYME nomenclature database: 3.4.23.18
BRENDA, the Enzyme Database: 3.4.23.18
CAS: 9025-49-4

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