Hydrolases;
Acting on peptide bonds (peptidases);
Aspartic endopeptidases
Reaction(IUBMB)
Hydrolysis of proteins with broad specificity similar to that of pepsin A, preferring hydrophobic residues at P1 and P1'. Clots milk and activates trypsinogen. Does not cleave Gln4-His, but does cleave His10!Leu and Val12!Glu in B chain of insulin
Comment
From the zygomycete fungus Rhizopus chinensis. A similar endopeptidase is found in R. niveus [2]. In peptidase family A1 (pepsin A family).
History
EC 3.4.23.21 created 1992 (EC 3.4.23.6 created 1992 (EC 3.4.23.6 created 1961 as EC 3.4.4.17, transferred 1972 to EC 3.4.23.6, modified 1981 [EC 3.4.23.7, EC 3.4.23.8, EC 3.4.23.9, EC 3.4.23.10, EC 3.4.99.1, EC 3.4.99.15 and EC 3.4.99.25 all created 1972 and incorporated 1978], part incorporated 1992)
Reference
1
Authors
Tsuru, D., Hattori, A., Tsuji, H., Yamamoto, T. and Fukumoto, J.
Title
Studies on mold proteases. Part II. Substrate specificity of acid protease of Rhizopus chinensis.
Journal
Agric Biol Chem 33:1419-1426 (1969)
Reference
2
Authors
Kurono, Y., Chidimatsu, M., Horikoshi, K. and Ikeda, Y.