Hydrolases;
Acting on peptide bonds (peptidases);
Aspartic endopeptidases
Reaction(IUBMB)
Hydrolysis of proteins with specificity similar to that of pepsin A, but also cleaves Cys(SO3H)7!Gly and Leu17!Val in the B chain of insulin
Comment
Isolated from the imperfect fungus Scytalidium lignicolum. Not inhibited by pepstatin-Ac, methyl 2-diazoacetamidohexanoate or 1,2-epoxy-3-(p-nitrophenyl)propane. A related enzyme from the same organism, proteinase C, is also insensitive to these inhibitors and has Mr = 406,000 [3]
History
EC 3.4.23.31 created 1992
Reference
1
Authors
Oda, K. and Murao, S.
Title
Purification and some enzymatic properties of acid protease A and B of Scytalidium lignicolum ATCC 24568.
Journal
Agric Biol Chem 38:2435-2444 (1974)
Reference
2
Authors
Oda, K. and Murao, S.
Title
Action of Scytalidium lignicolum acid proteases on insulin B-chain.
Journal
Agric Biol Chem 40:1221-1225 (1976)
Reference
3
Authors
Oda, K., Torishima, H. and Murao, S.
Title
Purification and characterization of acid proteinase C of Scytalidium lignicolum ATCC 24568.
