KEGG ENZYME: 3.4.24.15

ENZYME: 3.4.24.15

Entry

EC 3.4.24.15                Enzyme

Name

thimet oligopeptidase;
Pz-peptidase;
soluble metalloendopeptidase;
endo-oligopeptidase A;
tissue-endopeptidase degrading collagenase-synthetic-substrate

Class

Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases

Reaction(IUBMB)

Preferential cleavage of bonds with hydrophobic residues at P1, P2 and P3' and a small residue at P1' in substrates of 5-15 residues

Comment

Thiol compounds activate at low concentrations. Type example of peptidase family M3.

History

EC 3.4.24.15 created 1984 (EC 3.4.22.19 created 1989 and EC 3.4.99.31 created 1978 both incorporated 1992)

Orthology

K01392

thimet oligopeptidase

Genes

HSA:	7064(THOP1)

PTR:	750162(THOP1)

PPS:	100985112(THOP1)

GGO:	101143234(THOP1)

PON:	100455971(THOP1)

NLE:	100583449(THOP1)

HMH:	116483102(THOP1)

MCC:	721578(THOP1)

MCF:	102117812(THOP1)

MTHB:

126942541

MNI:	105485733(THOP1)

CSAB:

103233658(THOP1)

CATY:

105591760(THOP1)

PANU:

101016285(THOP1)

TGE:	112613114(THOP1)

MLEU:

105551973(THOP1)

RRO:	104665465(THOP1)

RBB:	108537535(THOP1)

TFN:	117074290(THOP1)

PTEH:

111520680(THOP1)

CANG:

105527655(THOP1)

CJC:	100385317(THOP1)

SBQ:	101028985(THOP1)

CIMI:

108288502(THOP1)

CSYR:

103265974(THOP1)

MMUR:

105873975(THOP1)

LCAT:

123633237(THOP1)

PCOQ:

105811943(THOP1)

OGA:	100947655(THOP1)

MMU:	50492(Thop1)

MCAL:

110303835(Thop1)

MPAH:

110326403(Thop1)

RNO:	64517(Thop1)

MCOC:

116076209(Thop1)

ANU:	117696806(Thop1)

MUN:	110555611(Thop1)

CGE:	100764672(Thop1)

MAUA:

101823587(Thop1)

PROB:

127216546(Thop1)

PLEU:

114688124(Thop1)

MORG:

121459922(Thop1)

MFOT:

126489301

AAMP:

119800920(Thop1)

NGI:	103737278(Thop1)

HGL:	101714143(Thop1)

CPOC:

100731879(Thop1)

CCAN:

109691048(Thop1) 109702423

DORD:

105990473(Thop1)

DSP:	122099291(Thop1)

PLOP:

125348632(Thop1)

NCAR:

124969480

MMMA:

107157425(Thop1)

OPI:	101521830(THOP1)

TUP:	102500342(THOP1)

GVR:	103605472(THOP1)

CFA:	476748(THOP1)

CLUD:

112666240(THOP1)

VVP:	112935469(THOP1)

VLG:	121494789(THOP1)

NPO:	129521739(THOP1)

AML:	100471700(THOP1)

UMR:	103681807(THOP1)

UAH:	113242770(THOP1)

UAR:	123793979(THOP1)

ELK:

111161713

LLV:

125086980

MPUF:

101675322(THOP1)

MNP:	132010756(THOP1)

MLK:	131823254(THOP1)

NVS:	122909904(THOP1)

ORO:	101362401(THOP1)

EJU:	114226166(THOP1)

ZCA:	113916363(THOP1)

MLX:	118005518(THOP1)

NSU:	110593679(THOP1)

LWW:	102733859(THOP1)

FCA:	101083746(THOP1)

PYU:	121021304(THOP1)

PBG:	122486659(THOP1)

PVIV:

125160661(THOP1)

LRUF:

124524840

PTG:	102964477(THOP1)

PPAD:

109260811(THOP1)

PUC:

125930507

AJU:

106982145

HHV:	120242394(THOP1)

BTA:	510889(THOP1)

BOM:	102265369(THOP1)

BIU:	109561783(THOP1)

BBUB:

102413536(THOP1)

BBIS:

105005586(THOP1)

CHX:	102178165(THOP1)

OAS:	101119557(THOP1)

BTAX:

128050814(THOP1)

ODA:	120864781(THOP1)

CCAD:

122440188(THOP1)

MBEZ:

129559136(THOP1)

SSC:	397442(THOP1)

CFR:	102508107(THOP1)

CBAI:

105078170(THOP1)

CDK:	105106052(THOP1)

VPC:	102539590(THOP1)

BACU:

103019210(THOP1)

BMUS:

118892444(THOP1) 118892597

LVE:	103090843(THOP1)

OOR:	101287363(THOP1)

DLE:	111170481(THOP1)

PCAD:

102992430(THOP1)

PSIU:

116751758(THOP1) 116761195

NASI:

112396373(THOP1)

ECB:	100059667(THOP1)

EPZ:	103540477(THOP1)

EAI:	106834154(THOP1)

MYB:	102262534(THOP1)

MYD:	102774039(THOP1)

MMYO:

118658947(THOP1)

MLF:	102426538(THOP1)

PKL:	118731308(THOP1)

EFUS:

103294259(THOP1)

MNA:	107534611(THOP1)

DRO:	112312636(THOP1)

SHON:

118984464(THOP1)

AJM:	119063770(THOP1)

PDIC:

114502924(THOP1)

PHAS:

123814089(THOP1)

MMF:	118615985(THOP1)

PPAM:

129081319(THOP1)

HAI:	109390675(THOP1)

RFQ:	117038291(THOP1)

PALE:

102886786(THOP1)

PGIG:

120583743(THOP1)

PVP:	105300464(THOP1)

RAY:	107520132(THOP1)

MJV:

108393267

TOD:	119240546(THOP1)

SARA:

101543766(THOP1)

SETR:

126031140(THOP1)

LAV:

100665776

TMU:

101346830

ETF:	101649445(THOP1)

DNM:	101436326(THOP1)

MDO:	100020499(THOP1)

GAS:	123256779(THOP1)

SHR:	100924623(THOP1)

AFZ:

127545129

PCW:	110223381(THOP1)

OAA:	100076730(THOP1)

GGA:	426398(THOP1)

PCOC:

116235780(THOP1)

MGP:	100545913(THOP1)

CJO:	107325439(THOP1)

TPAI:

128088932(THOP1)

LMUT:

125685058(THOP1)

NMEL:

110388975(THOP1)

APLA:

101804753(THOP1)

ACYG:

106048201(THOP1)

CATA:

118260844(THOP1)

AFUL:

116499110(THOP1)

TGU:	100231983(THOP1)

LSR:	110483736(THOP1)

SCAN:

103822058(THOP1)

PMOA:

120513634(THOP1)

OTC:	121342122(THOP1)

PRUF:

121360908

GFR:	102032301(THOP1)

FAB:	101805763(THOP1)

OMA:	130264551(THOP1)

PHI:	102110662(THOP1)

PMAJ:

107215455(THOP1)

CCAE:

111940251(THOP1)

CCW:	104697073(THOP1)

CBRC:

103622198

ETL:	114066386(THOP1)

ZAB:	102062983(THOP1)

ACHL:

103804792(THOP1)

SVG:	106857154(THOP1)

MMEA:

130580196(THOP1)

FPG:	101913462(THOP1)

FCH:	102053721(THOP1)

CLV:	102095097(THOP1)

EGZ:	104132299(THOP1)

NNI:	104016288(THOP1)

PCRI:

104035619(THOP1)

PLET:

104621712(THOP1)

EHS:	104513053(THOP1)

PCAO:

104042347(THOP1)

ACUN:

113489333(THOP1)

TALA:

104363459(THOP1)

PADL:

103925937(THOP1)

AFOR:

103899398(THOP1)

ACHC:

115348846(THOP1)

HALD:

104319119

HLE:	104829105(THOP1)

AGEN:

126041611

GCL:

127025543

CCRI:

104165370(THOP1)

CSTI:

104554122

CMAC:

104474322(THOP1)

MUI:	104539560(THOP1)

BREG:

104641176(THOP1)

FGA:	104084170(THOP1)

GSTE:

104260085(THOP1)

LDI:

104342201

MNB:	103780001(THOP1)

OHA:	104327957(THOP1)

NNT:	104413388(THOP1)

SHAB:

115618044(THOP1)

DPUB:

104299056(THOP1)

PGUU:

104471466(THOP1)

ACAR:

104523453

CPEA:

104395609(THOP1)

AVIT:

104279925(THOP1)

CVF:	104285119(THOP1)

RTD:	128900752(THOP1)

CUCA:

104065758(THOP1)

TEO:	104378215(THOP1)

AAM:	106486695(THOP1)

AROW:

112965657(THOP1)

NPD:	112946888(THOP1)

TGT:	104568357(THOP1)

DNE:	112992759(THOP1)

SCAM:

104139383(THOP1)

ASN:	102383675(THOP1)

AMJ:	102558370(THOP1)

CPOO:

109312321(THOP1)

GGN:	109294955(THOP1)

PSS:	102457370(THOP1)

CMY:	102940710(THOP1)

CCAY:

125627075(THOP1)

DCC:	119848370(THOP1)

CPIC:

101943510(THOP1)

TST:	117868787(THOP1)

CABI:

116831649(THOP1)

MRV:	120391749(THOP1)

ASAO:

132780866(THOP1)

PVT:	110079254(THOP1)

SUND:

121936693(THOP1)

PBI:	103051765(THOP1)

PMUR:

107296844(THOP1)

CTIG:

120299507(THOP1)

TSR:	106544855(THOP1)

PGUT:

117670577(THOP1)

APRI:

131188456(THOP1)

PTEX:

113451175(THOP1)

NSS:	113426270(THOP1)

VKO:

123017592

PMUA:

114588367(THOP1)

PRAF:

128405630(THOP1)

ZVI:	118087077(THOP1)

HCG:	128344541(THOP1)

GJA:	107105512(THOP1)

STOW:

125433653(THOP1)

EMC:	129330744(THOP1)

XLA:	108706839(thop1.S) 397806(thop1.L)

XTR:	779863(thop1)

NPR:

108802730

RTEM:

120912800(THOP1)

BBUF:

120989474(THOP1)

BGAR:

122919913(THOP1)

MUO:	115480489(THOP1)

GSH:	117365613(THOP1)

DRE:	436628(thop1)

SRX:	107726548 107730481 107751012

SANH:

107658499

SGH:	107568058 107592556

CCAR:

109087313

CAUA:

113054995 113069380

CGIB:

127968090 128022613

PTET:

122354205(thop1)

LROH:

127173375(thop1)

OMC:	131549863(thop1)

PPRM:

120464244(thop1)

RKG:	130100915(thop1)

MAMB:

125256422(thop1)

CIDE:

127522016

MASI:

127426443 127427641

TROS:

130571149(thop1)

TDW:	130410790(thop1)

MANU:

129427250(thop1)

IPU:

108271294

IFU:	128614035(thop1)

PHYP:

113535897(thop1)

SMEO:

124391613(thop1)

TFD:	113657854(thop1)

TVC:	132851924(thop1)

AMEX:

103038939(thop1)

CMAO:

118819360(thop1)

EEE:	113568245(thop1)

CHAR:

105896439(thop1)

TRU:

101069017

TFS:	130528297(thop1)

TNG:	GSTEN00006269G001

LCO:	104935746(thop1)

TBEN:

117491064(thop1)

CGOB:

115006745(thop1)

PGEO:

117445633(thop1)

GACU:

117534328(thop1)

EMAC:

134869686(thop1)

ELY:	117254224(thop1)

EFO:	125895982(thop1)

PLEP:

121941066(thop1)

SLUC:

116066747(thop1)

ESP:

116679471

PFLV:

114552158(thop1)

GAT:	120823858(thop1)

PPUG:

119216330(thop1)

AFB:	129094302(thop1)

CLUM:

117730033(thop1)

PSWI:

130193560(thop1)

MSAM:

119888385 119888386

SCHU:

122877413(thop1)

CUD:	121511696(thop1)

ALAT:

119029193(thop1)

MZE:	101464999(thop1)

ONL:

100703187

OAU:	116323245(thop1)

OLA:	101157763(thop1)

OML:	112136800(thop1)

CSAI:

133458331(thop1)

XMA:	102222331(thop1)

XCO:	114141368(thop1)

XHE:

116725751

PRET:

103463257(thop1)

PFOR:

103155047(thop1)

PLAI:

106941416(thop1)

PMEI:

106927773(thop1)

GAF:	122838058(thop1)

PPRL:

129378906(thop1)

CVG:	107085901(thop1)

CTUL:

119791252(thop1)

GMU:	124873733(thop1)

NFU:	107395897(thop1)

KMR:	108228871(thop1)

ALIM:

106532164

NWH:	119430462(thop1)

AOCE:

111563975(thop1)

MCEP:

125009784(thop1)

CSEM:

103396631(thop1)

POV:

109642545

SSEN:

122780778(thop1)

HHIP:

117760170(thop1)

HSP:	118121258(thop1)

SMAU:

118318066(thop1)

LCF:	108882357(thop1) 108892667

SDU:	111231825(thop1)

SLAL:

111671759(thop1)

XGL:	120790957(thop1)

HCQ:	109506833(thop1)

SSCV:

125975091

SBIA:

133503286(thop1)

PEE:	133406741(thop1)

PTAO:

133481261(thop1)

BPEC:

110167856(thop1)

MALB:

109965204(thop1)

BSPL:

114854329

SJO:	128362053(thop1)

SASA:

100194867(mepd) 106613599(MEPD)

STRU:

115151639 115158073

OTW:	112250709 112252097

OMY:	110489937 110524109

OGO:	123997264(thop1) 123997863

ONE:	115108147 115109114

OKI:	109889094 109902350

OKE:	118387838 118401487

SALP:

111952854 111975950

SNH:	120053694 120054667

CCLU:

121533688 121576477(thop1)

ELS:	105011365(thop1)

SFM:

108935234

PKI:

111840410

AANG:

118225034(thop1)

LOC:

102698056

PSPA:

121300806

ARUT:

117401455 117966344

PSEX:

120537589(thop1)

LCM:	102357708(THOP1)

CMK:

103181955

RTP:

109912733

CPLA:

122557939 122565420

HOC:	132821387 132828857

LERI:

129711114(thop1)

PMRN:

116942290(THOP1)

LRJ:

133359165

BFO:

118426054

BBEL:

109463730

CIN:

100183579

SCLV:

120345308

SPU:

585276

LPIC:

129271765 129271768

APLC:

110987267

AJC:

117106427

SKO:

100373471

BCOO:

119080155 119080156

FCD:

110858459

PVM:

113824454

PJA:

122251371

PCHN:

125027779

PMOO:

119572960

HAME:

121868559

PCLA:

123762978

PTRU:

123517768

ESN:

127005818

MNZ:

135195550

HAZT:

108669727

EAF:

111710536

TCF:

131886280

PPOI:

119101446

DSV:

119433527

RSAN:

119405166 119405167

RMP:

119181934

CSCU:

111634737 111637553 111642625

PTEP:

107452832

ABRU:

129975725

UDV:

129216964

SDM:	118187678 118200824

LPOL:

106458305

LGI:	LOTGIDRAFT_178669

PVUL:

126819247

PCAN:

112573585

BGT:

106064971

GAE:	121376297 121376298

HRF:

124113096

HRJ:

124258522

CRG:	105326160 117684168

CVN:	111117774 111122414

CANU:

128164619

OED:	125668093 125668094

MYI:

110466585

PMAX:

117325603

MCAF:

127735844

MMER:

123524249

RPHI:

132719648

DPOL:

127833413

OBI:

106883272

OSN:

115222172

LAK:

106165041

SMM:	Smp_029470 Smp_029500

SHX:	MS3_00004895(THOP1_1) MS3_00004897(THOP1_2)

OVI:	T265_02526

NVE:

5520300

EPA:

110233802

ATEN:

116297513

AMIL:

114975087

PDAM:

113674458

SPIS:

111336744

DGT:

114526239

XEN:

124436297

HMG:

100212290

TAD:	TRIADDRAFT_22587

ATH:

AT1G67690

ALY:

9324680

CRB:

17894312

CSAT:

104704713 104750568 104787784

EUS:	EUTSA_v10018203mg

BRP:	103831014 103831191

BNA:	106353963 106410390 106436805 106454291

BOE:	106300211 106300276

RSZ:	108817546 108817732

THJ:

104825527

CPAP:

110811640

CIT:

102612794

CIC:	CICLE_v10033657mg

PVY:

116128487

MINC:

123214879

TCC:

18609680

GRA:

105794549

GHI:	107886507 107909759

GAB:

108461139

HSYR:

120134494

DZI:

111282553

EGR:

104433638

GMX:	100793518 100796889

GSJ:

114394812

PVU:	PHAVU_001G258500g

VRA:

106755630

VAR:

108327003

VUN:

114176336

VUM:

124840004

CCAJ:

109809646

APRC:

113850421

MTR:

11446411

TPRA:

123910196

CAM:

101492891

PSAT:

127083060

VVO:	131661506 131661518

LJA:	Lj2g3v1402690.2(Lj2g3v1402690.2)

ADU:

107468942

AIP:

107623713

AHF:	112715069 112783859

LANG:

109327233

PCIN:

129293194

QSA:	O6P43_023901

FVE:

101295009

RCN:

112198397

PPER:

18789331

PMUM:

103321960

PAVI:

110753096

PDUL:

117616153

MDM:	103450130 103452091

MSYL:

126597794 126608737

PXB:

103949886

ZJU:

107428397

MNT:

21410202

CSAV:

115717346

CSV:

101203244

CMO:

103487749

BHJ:

120076088

MCHA:

111004893

CMAX:

111491781

CMOS:

111462816

CPEP:

111805736

RCU:

8278432

JCU:

105644751

HBR:

110640451

MESC:

110628853

POP:

7460043

PEU:

105138030

PALZ:

118048831

JRE:

108982025

CILL:

122314418

CAVE:

132179893

QSU:

112015579

QLO:

115982332

TWL:

119992207

VVI:

100251085

VRI:

117922528

SLY:

101244701

SPEN:

107030536

SOT:

102581011

SSTN:

125870859

SDUL:

129882459

CANN:

107878381

LBB:

132631303

NTA:	107776157 107805230

NSY:

104236568

NTO:

104101862

NAU:

109240158

INI:

109172014

ITR:

116024900

SIND:

105168364

OEU:

111401948

EGT:

105970919

SSPL:

121794523

SMIL:

130995561

SHIS:

125205300

APAN:

127241511

HAN:

110895102

ECAD:

122607332

LSV:

111887622

CCAV:

112524158

DCR:

108197056

CSIN:

114318340

RVL:

131311514

AEW:

130772416

BVG:

104897646

SOE:

110793856

CQI:	110710335 110712431

ATRI:

130809249

MOF:

131144688

NNU:

104591627

MING:

122078158

TSS:

122671950

PSOM:

113298209 113302654

OSA:

4325012

DOSA:

Os01t0902200-01(Os01g0902200)

OBR:

102721983

OGL:

127780107

BDI:

100821906

ATS:

109754044

TDC:	119269828 119277834

TAES:

123062821 123071663 123080010

TUA:

125545482

LPER:

127344991

LRD:

124661406

SBI:

8056125

ZMA:

100280152

SITA:

101785951

SVS:

117857165

PVIR:

120676599 120708956

PHAI:

112891681

PDA:

103713620

EGU:

105053005

MUS:

103994127

ZOF:	122041905 122046429

DCT:

110099372

PEQ:

110021067

AOF:

109836686

MSIN:

131246242

NCOL:

116250655

ATR:

18441855

SMO:	SELMODRAFT_113618 SELMODRAFT_164651

PPP:	112295568 112295983

MNG:	MNEG_12125

OLU:	OSTLU_46518

OTA:	OT_ostta09g02980

BPG:	Bathy10g01530

CME:	CYME_CMS446C

ZTR:	MYCGRDRAFT_107351

MBR:	MONBRDRAFT_25656

SRE:	PTSG_13259

DDI:	DDB_G0292362

DPP:	DICPUDRAFT_54379

DFA:

DFA_04384

AAF:	AURANDRAFT_72404

PIF:	PITG_08599

PSOJ:

PHYSODRAFT_329905

SPAR:

SPRG_12685

GTT:	GUITHDRAFT_166948

TBR:	Tb927.7.190

TBG:	TbgDal_VII70

TCR:	506411.10 511237.10

LMA:	LMJF_26_1570

LIF:	LINJ_26_1550

LDO:	LDBPK_261550

LMI:	LMXM_26_1570

LBZ:	LBRM_26_1590

LPAN:

LPMP_261550

NGR:	NAEGRDRAFT_36811

» show all

Reference

1 [PMID:3244563]

Authors

Cicilini MA, Ribeiro MJ, de Oliveira EB, Mortara RA, de Camargo AC.

Title

Endooligopeptidase A activity in rabbit heart: generation of enkephalin from enkephalin containing peptides.

Journal

Peptides 9:945-55 (1988)
DOI:10.1016/0196-9781(88)90072-1

Reference

2 [PMID:2803255]

Authors

Orlowski M, Reznik S, Ayala J, Pierotti AR.

Title

Endopeptidase 24.15 from rat testes. Isolation of the enzyme and its specificity toward synthetic and natural peptides, including enkephalin-containing peptides.

Journal

Biochem J 261:951-8 (1989)
DOI:10.1042/bj2610951

Reference

3 [PMID:2123097]

Authors

Barrett AJ, Brown MA.

Title

Chicken liver Pz-peptidase, a thiol-dependent metallo-endopeptidase.

Journal

Biochem J 271:701-6 (1990)
DOI:10.1042/bj2710701

Reference

4 [PMID:2261476]

Authors

Pierotti A, Dong KW, Glucksman MJ, Orlowski M, Roberts JL

Title

Molecular cloning and primary structure of rat testes metalloendopeptidase EC 3.4.24.15.

Journal

Biochemistry 29:10323-9 (1990)
DOI:10.1021/bi00497a006

Sequence

[rno:64517]

Reference

5 [PMID:2185743]

Authors

Tisljar U, Barrett AJ.

Title

Thiol-dependent metallo-endopeptidase characteristics of Pz-peptidase in rat and rabbit.

Journal

Biochem J 267:531-3 (1990)
DOI:10.1042/bj2670531

Other DBs

ExplorEnz - The Enzyme Database:

3.4.24.15

IUBMB Enzyme Nomenclature:

3.4.24.15

ExPASy - ENZYME nomenclature database:

3.4.24.15

BRENDA, the Enzyme Database:

3.4.24.15

CAS:	110639-28-6

DBGET integrated database retrieval system