KEGG ENZYME: 3.4.24.27

ENZYME: 3.4.24.27

Entry

EC 3.4.24.27                Enzyme

Name

thermolysin;
Bacillus thermoproteolyticus neutral proteinase;
thermoase;
thermoase Y10;
TLN

Class

Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases

Reaction(IUBMB)

Preferential cleavage: !Leu > !Phe

Comment

A thermostable extracellular metalloendopeptidase containing four calcium ions. Enzymes that may be species variants of thermolysin are reported from Micrococcus caseolyticus [4] and Aspergillus oryzae [5]. Type example of peptidase family M4. Closely related but distinct enzymes are aeromonolysin, pseudolysin, bacillolysin, aureolysin and mycolysin

History

EC 3.4.24.27 created 1972 as EC 3.4.24.4, part transferred 1992 to EC 3.4.24.27

Orthology

K08603

thermolysin

Genes

HAU:

Haur_3645

Reference

1 [PMID:5954368]

Authors

Ohta Y, Ogura Y, Wada A.

Title

Thermostable protease from thermophilic bacteria. I. Thermostability, physiocochemical properties, and amino acid composition.

Journal

J Biol Chem 241:5919-25 (1966)

Reference

2 [PMID:4967801]

Authors

Morihara K, Tsuzuki H, Oka T.

Title

Comparison of the specificities of various neutral proteinases from microorganisms.

Journal

Arch Biochem Biophys 123:572-88 (1968)
DOI:10.1016/0003-9861(68)90179-3

Reference

3 [PMID:5823940]

Authors

Latt SA, Holmquist B, Vallee BL

Title

Thermolysin: a zinc metalloenzyme.

Journal

Biochem Biophys Res Commun 37:333-9 (1969)
DOI:10.1016/0006-291X(69)90739-6

Reference

4 [PMID:5551628]

Authors

Desmazeaud MJ, Hermier JH.

Title

[Specificity of the nuclear protease produced by Micrococcus caseolyticus]

Journal

Eur J Biochem 19:51-5 (1971)
DOI:10.1111/j.1432-1033.1971.tb01286.x

Reference

5 [PMID:5004124]

Authors

Morihara K, Tsuzuki H.

Title

Comparative study of various neutral proteinases from microorganisms: specificity with oligopeptides.

Journal

Arch Biochem Biophys 146:291-6 (1971)
DOI:10.1016/S0003-9861(71)80066-8

Reference

6 [PMID:18663848]

Authors

Titani K, Hermodson MA, Ericsson LH, Walsh KA, Neurath H

Title

Amino-acid sequence of thermolysin.

Journal

Nat New Biol 238:35-7 (1972)
DOI:10.1038/newbio238035a0

Sequence

[ag:CAA54291]

Reference

Authors

Matthews, B.

Title

W. Structural basis of the action of thermolysin and related zinc peptidases.

Journal

Acc Chem Res 21:333-340 (1988)

Other DBs

ExplorEnz - The Enzyme Database:

3.4.24.27

IUBMB Enzyme Nomenclature:

3.4.24.27

ExPASy - ENZYME nomenclature database:

3.4.24.27

BRENDA, the Enzyme Database:

3.4.24.27

CAS:

9073-78-3

DBGET integrated database retrieval system