KEGG ENZYME: 3.4.24.38

ENZYME: 3.4.24.38

Entry

EC 3.4.24.38                Enzyme

Name

gametolysin;
autolysin;
Chlamydomonas cell wall degrading protease;
lysin;
Chlamydomonas reinhardtii metalloproteinase;
gamete lytic enzyme;
gamete autolysin

Class

Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases

Reaction(IUBMB)

Cleavage of the proline- and hydroxyproline-rich proteins of the Chlamydomonas cell wall; also cleaves azocasein, gelatin and Leu-Trp-Met!Arg-Phe-Ala

Comment

A glycoprotein found in the periplasmic space of Chlamydomonas reinhardtii gametes in a 62 kDa inactive form; decreased to 60 kDa upon activation. A zinc enzyme, inhibited by phosphoramidon, but also thiol activated. Type example of peptidase family M11

History

EC 3.4.24.38 created 1992, modified 2000

Reference

1 [PMID:3319620]

Authors

Jaenicke L, Kuhne W, Spessert R, Wahle U, Waffenschmidt S.

Title

Cell-wall lytic enzymes (autolysins) of Chlamydomonas reinhardtii are (hydroxy)proline-specific proteases.

Journal

Eur J Biochem 170:485-91 (1987)
DOI:10.1111/j.1432-1033.1987.tb13725.x

Reference

2 [PMID:2910877]

Authors

Buchanan MJ, Imam SH, Eskue WA, Snell WJ.

Title

Activation of the cell wall degrading protease, lysin, during sexual signalling in Chlamydomonas: the enzyme is stored as an inactive, higher relative molecular mass precursor in the periplasm.

Journal

J Cell Biol 108:199-207 (1989)
DOI:10.1083/jcb.108.1.199

Reference

Authors

Matsuda, Y.

Title

Gametolysin.

Journal

In: Barrett, A.J., Rawlings, N.D. & Woessner, J.F. (Eds.), Handbook of Proteolytic Enzymes, Academic Press, London, 1998, p. 1140-1143.

Other DBs

ExplorEnz - The Enzyme Database:

3.4.24.38

IUBMB Enzyme Nomenclature:

3.4.24.38

ExPASy - ENZYME nomenclature database:

3.4.24.38

BRENDA, the Enzyme Database:

3.4.24.38

CAS:	97089-74-2

All links

Protein sequence (3)   
   UniProt (2)   
   SWISS-PROT (1)   
DNA sequence (6)   
   GenBank (3)   
   EMBL (3)   
Protein domain (3)   
   InterPro (3)   
All databases (12)   

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