KEGG   ENZYME: 3.4.24.74
Entry
EC 3.4.24.74                Enzyme                                 
Name
fragilysin;
Bacteroides fragilis (entero)toxin
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
Reaction(IUBMB)
Broad proteolytic specificity, bonds hydrolysed including -Gly!Leu-, -Met!Leu-, -Phe!Leu-, -Cys!Leu-, Leu!Gly
Comment
Thought to be a cause of diarrhoea in animals and humans. Hydrolyses extracellular matrix proteins, and disrupts tight junctions of intestinal epithelial cells. Also degrades intracellular, cytoskeletal proteins actin, myosin and others. In peptidase family M10 (interstitial collagenase family)
History
EC 3.4.24.74 created 1997
Orthology
K19959  fragilysin
Genes
BFBVU15_14325 VU15_18690
Reference
1  [PMID:7806355]
  Authors
Moncrief JS, Obiso R Jr, Barroso LA, Kling JJ, Wright RL, Van Tassell RL, Lyerly DM, Wilkins TD.
  Title
The enterotoxin of Bacteroides fragilis is a metalloprotease.
  Journal
Infect Immun 63:175-81 (1995)
DOI:10.1128/IAI.63.1.175-181.1995
Reference
2  [PMID:7558286]
  Authors
Obiso RJ Jr, Lyerly DM, Van Tassell RL, Wilkins TD.
  Title
Proteolytic activity of the Bacteroides fragilis enterotoxin causes fluid secretion and intestinal damage in vivo.
  Journal
Infect Immun 63:3820-6 (1995)
DOI:10.1128/IAI.63.10.3820-3826.1995
Reference
3  [PMID:8557328]
  Authors
Donelli G, Fabbri A, Fiorentini C.
  Title
Bacteroides fragilis enterotoxin induces cytoskeletal changes and surface blebbing in HT-29 cells.
  Journal
Infect Immun 64:113-9 (1996)
DOI:10.1128/IAI.64.1.113-119.1996
Reference
4  [PMID:8945541]
  Authors
Koshy SS, Montrose MH, Sears CL.
  Title
Human intestinal epithelial cells swell and demonstrate actin rearrangement in response to the metalloprotease toxin of Bacteroides fragilis.
  Journal
Infect Immun 64:5022-8 (1996)
DOI:10.1128/IAI.64.12.5022-5028.1996
Reference
5  [PMID:9011050]
  Authors
Kling JJ, Wright RL, Moncrief JS, Wilkins TD
  Title
Cloning and characterization of the gene for the metalloprotease enterotoxin of Bacteroides fragilis.
  Journal
FEMS Microbiol Lett 146:279-84 (1997)
DOI:10.1111/j.1574-6968.1997.tb10205.x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.4.24.74
IUBMB Enzyme Nomenclature: 3.4.24.74
ExPASy - ENZYME nomenclature database: 3.4.24.74
BRENDA, the Enzyme Database: 3.4.24.74
CAS: 188596-63-6

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