KEGG ENZYME: 3.5.1.117

ENZYME: 3.5.1.117

Entry

EC 3.5.1.117                Enzyme

Name

6-aminohexanoate-oligomer endohydrolase;
endo-type 6-aminohexanoate oligomer hydrolase;
Ahx endo-type-oligomer hydrolase;
6-aminohexanoate oligomer hydrolase;
Ahx-oligomer hydrolase;
nylon hydrolase;
nylon-oligomer hydrolase;
NylC;
nylon-6 hydrolase (ambiguous)

Class

Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides

Sysname

6-aminohexanoate oligomer endoamidohydrolase

Reaction(IUBMB)

[N-(6-aminohexanoyl)]n + H2O = [N-(6-aminohexanoyl)]n-x + [N-(6-aminohexanoyl)]x [RN:R10976]

Reaction(KEGG)

R10976;
(other) R10978 R10979

Substrate

[N-(6-aminohexanoyl)]n [CPD:C20988];
H2O [CPD:C00001]

Product

[N-(6-aminohexanoyl)]n-x;
[N-(6-aminohexanoyl)]x

Comment

The enzyme is involved in degradation of nylon-6 oligomers. It degrades linear or cyclic oligomers of poly(6-aminohexanoate) with a degree of polymerization greater than three (n > 3) by endo-type cleavage, to oligomers of a length of two or more (2 <= x < n). It shows negligible activity with N-(6-aminohexanoyl)-6-aminohexanoate (cf. EC 3.5.1.46, 6-aminohexanoate-oligomer exo hydrolase) or with 1,8-diazacyclotetradecane-2,9-dione (cf. EC 3.5.2.12, 6-aminohexanoate-cyclic-dimer hydrolase).

History

EC 3.5.1.117 created 2014

Pathway

ec00930

Caprolactam degradation

ec01120

Microbial metabolism in diverse environments

Orthology

K19963

6-aminohexanoate-oligomer endohydrolase

Genes

GSO:	PH603_12665

MICR:

BMW26_04430

MRN:	K8F61_06370

MICS:

C1N74_01735

ASOI:

MTP13_02070 MTP13_02075 MTP13_02450

LTR:	EVS81_05135

LRP:	MUN76_07125

AGG:	C1N71_00300

GSD:	M3M28_12475

SGRG:

L0C25_05825

ACTW:

F7P10_35555

NOA:	BKM31_31370

NGN:	LCN96_13830

NFS:	OIE67_33265

AMYY:

YIM_16760

PMAD:

BAY61_11955

MICH:

FJK98_13105

MCAB:

HXZ27_01495

MENO:

Jiend_13290

CALN:

NIES2098_53370

» show all

Reference

1 [PMID:8285701]

Authors

Kakudo S, Negoro S, Urabe I, Okada H

Title

Nylon oligomer degradation gene, nylC, on plasmid pOAD2 from a Flavobacterium strain encodes endo-type 6-aminohexanoate oligomer hydrolase: purification and characterization of the nylC gene product.

Journal

Appl Environ Microbiol 59:3978-80 (1993)
DOI:10.1128/AEM.59.11.3978-3980.1993

Reference

2 [PMID:17827307]

Authors

Yasuhira K, Tanaka Y, Shibata H, Kawashima Y, Ohara A, Kato D, Takeo M, Negoro S

Title

6-Aminohexanoate oligomer hydrolases from the alkalophilic bacteria Agromyces sp. strain KY5R and Kocuria sp. strain KY2.

Journal

Appl Environ Microbiol 73:7099-102 (2007)
DOI:10.1128/AEM.00777-07

Reference

3 [PMID:22187439]

Authors

Negoro S, Shibata N, Tanaka Y, Yasuhira K, Shibata H, Hashimoto H, Lee YH, Oshima S, Santa R, Oshima S, Mochiji K, Goto Y, Ikegami T, Nagai K, Kato D, Takeo M, Higuchi Y

Title

Three-dimensional structure of nylon hydrolase and mechanism of nylon-6 hydrolysis.

Journal

J Biol Chem 287:5079-90 (2012)
DOI:10.1074/jbc.M111.321992

Other DBs

ExplorEnz - The Enzyme Database:

3.5.1.117

IUBMB Enzyme Nomenclature:

3.5.1.117

ExPASy - ENZYME nomenclature database:

3.5.1.117

BRENDA, the Enzyme Database:

3.5.1.117

DBGET integrated database retrieval system