KEGG ENZYME: 3.5.1.131

ENZYME: 3.5.1.131

Entry

EC 3.5.1.131                Enzyme

Name

1-carboxybiuret hydrolase;
atzEG (gene names)

Class

Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides

Sysname

1-carboxybiuret amidohydrolase

Reaction(IUBMB)

1-carboxybiuret + H2O = urea-1,3-dicarboxylate + NH3 [RN:R12333]

Reaction(KEGG)

R12333

Substrate

1-carboxybiuret [CPD:C22084];
H2O [CPD:C00001]

Product

urea-1,3-dicarboxylate [CPD:C22085];
NH3 [CPD:C00014]

Comment

The enzyme, characterized from the bacterium Pseudomonas sp. ADP, participates in the degradation of cyanuric acid, an intermediate in the degradation of s-triazine herbicides such as atrazine [2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine]. The enzyme is a heterotetramer composed of a catalytic subunit (AtzE) and an accessory subunit (AtzG) that stabilizes the complex.

History

EC 3.5.1.131 created 2019

Pathway

ec00791

Atrazine degradation

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K19837

1-carboxybiuret hydrolase

Genes

XHY:	FZ025_14215

TTU:	TERTU_2008

HNA:	Hneap_0846

BMEC:

WJ16_29720

BCAI:

K788_0005134

PHS:	C2L64_35110

PTER:

C2L65_31040

PPNO:

DA70_11690

PRB:	X636_15140

BUO:	BRPE64_CCDS07460

CABK:

NK8_46990

BAV:

BAV2584

BHO:

D560_0088

BHM:

D558_0086

BHZ:	ACR54_01203(atzE_1)

BGM:	CAL15_12200

BGV:	CAL12_04115

AXO:	NH44784_062021

AXN:	AX27061_4440

AXX:	ERS451415_04465(gatA_6)

ADT:	APT56_20365

AIS:	BUW96_12085

ASW:	CVS48_24170

ACHR:

C2U31_04985 C2U31_20980

ACHB:

DVB37_18575

ADY:	HLG70_01270

ACHO:

H4P35_17860

AMUI:

PE062_14960

APES:

FOC84_22940

AAEG:

RA224_10190

RHY:	RD110_11425

RHOB:

HTY51_12990

POS:	DT070_08305

POO:	F7R28_01110

PVAC:

HC248_02177(atzE)

VAA:	AX767_09315

VAM:	C4F17_17450

CSER:

CCO03_17440

AANT:

HUK68_18810

HYR:	BSY239_3270

HYB:

Q5W_18935

HTN:	KI616_25550

HCZ:	G9Q37_14570

HGR:	DW355_00365

MPT:

Mpe_A0772

METP:

C1M51_12975

ATER:

MW290_07220

LCH:

Lcho_3028

RGU:	A4W93_06100

THI:

THI_3642

XYK:	GT347_08325 GT347_14710

XYG:	R9X41_03455 R9X41_06435

URU:	DSM104443_03830(atzE)

BEBA:

BWI17_07860

BJA:	blr1041(blr1041)

BJU:	BJ6T_10660

BJP:	RN69_05200

BRA:

BRADO6818

BBT:

BBta_0721

BRS:

S23_67650

AOL:

S58_06460

BRC:	BCCGELA001_34450

BIC:	LMTR13_36505

BRO:	BRAD285_6962

BRK:	CWS35_10030

BOT:	CIT37_09095

BRQ:	CIT40_30240

BGQ:	X265_34830

BGZ:	XH91_33195

BSYM:

CIT39_29345

BBET:

F8237_13180

BARH:

WN72_46375

BVZ:	BRAD3257_8686(atzE)

BEL:	BE61_90570

BDG:	LPJ38_02320

BSEP:

HAP48_0012080

BQB:	J4P68_0015935

BSEI:

KMZ68_03325

BBAN:

J4G43_050875

BCAN:

BcanWSM471_35620

BAUT:

QA635_04710

BBRA:

QA636_04185

BGK:	IC762_32635

BCOU:

IC761_34810

BPAH:

QA639_04130

BXN:	I3J27_37765

BROS:

QUH67_33780

BRAZ:

LRP30_02170

RPA:	TX73_007545

RPB:

RPB_4054

RPT:

Rpal_1654

RPX:	Rpdx1_4053

RBK:	E0H22_19440

TRB:	HB776_22485

TALZ:

RPMA_27335

BOP:	AXW83_10740

BOS:	BSY19_3606

BVV:	BHK69_25585

BOF:	FQV39_02795

XAU:

Xaut_4656

XDI:	EZH22_18250

AZC:

AZC_1515

STAR:

G3545_10200

LNE:	FZC33_33270

SNO:

Snov_0644

ANC:	GBB76_00270

APRA:

G3A50_16055

APOL:

K9D25_20760

MDI:

METDI2366

MEX:

Mext_1693

MCH:

Mchl_2012

MPO:

Mpop_1125

MZA:	B2G69_16135

MRD:	Mrad2831_0653

MET:

M446_2141

MOR:

MOC_0819

META:

Y590_08125

MAQU:

Maq22A_1p30080(gatA)

MPHY:

MCBMB27_00237

METX:

A3862_27655

METS:

DK389_14765

METI:

DK427_22005

MMES:

MMSR116_27645

MIND:

mvi_23620

MOG:	MMB17_22975

MTAD:

M6G65_12940

MOC:	BB934_31690

MTUN:

MTUNDRAET4_1077

MLG:	CWB41_07105 CWB41_09725

BBAR:

RHAL1_03705(atzE)

HNI:	W911_10855

RHZ:	RHPLAN_22410

CMET:

K6K41_00335

PLEO:

OHA_1_04339(atzE)

HDI:	HDIA_3392(atzE)

BRN:	D1F64_14005

PSIN:

CAK95_16955

RBM:

TEF_15430

LAP:	ACP90_12380

LABR:

CHH27_08085

LABT:

FIU93_18170(atzE)

LAGG:

B0E33_24320

PHR:	C6569_12325

PSTG:

E8M01_05740

PAQT:

E8L99_00370

LIZ:	LGH83_07240

ACUT:

MRB58_12275

CCR:

CC_2613

CCS:	CCNA_02696

CAK:

Caul_3713

CSE:

Cseg_1082

CHQ:	AQ619_04460

CFH:	C1707_01505

CAUF:

CSW63_07655

TPRO:

Ga0080559_TMP448

AHT:	ANTHELSMS3_01752

PPRU:

FDP22_20460

PGV:	SL003B_1961

NPP:	PP1Y_Mpl6449

NHUM:

PQ457_14075

SPHI:

TS85_12020

GOH:

B932_0815

GTI:	FXF46_06895

GXY:

GLX_26950

GXL:

H845_638

KNA:	B0W47_13580

KEU:	S101446_03028

KSC:	CD178_00377(atzE_1)

KRE:	GWK63_04125

AACE:

A0U92_07820

APER:

A0U91_00650

ABG:	Asbog_01725

KBA:	A0U89_04070

RFL:

Rmf_17390

NTN:	D5366_09690

LCK:	HN018_07495

SHUM:

STHU_04700

SVC:	STVA_06280

STEL:

STAQ_39470

AZL:	AZL_a09080(gatA)

AZS:	E6C72_15835

SKT:	IGS68_23135

SMUC:

JL100_024760

SROE:

JL101_001890

FER:	FNB15_09965

THAR:

T8K17_00200

AFR:

AFE_0782

AFE:	Lferr_0925

ACU:

Atc_m027

AFI:	Acife_0656

AFJ:	AFERRID_20140

HFV:

R50_1286

RHOD:

AOT96_06825

KFL:

Kfla_7051

NOW:	GBF35_20750

NML:

Namu_0017

AMQ:	AMETH_4752(gatA)

PSEA:

WY02_17970

PSEE:

FRP1_24810

PSEH:

XF36_23605

PSEQ:

AD006_03995

PECQ:

AD017_11815

PHH:	AFB00_09665

PAUT:

Pdca_03620

CYA:

CYA_2100

CYB:

CYB_2659

DSL:	Dacsa_2233

AMR:

AM1_2033

LEN:	LEP3755_41510

LET:	O77CONTIG1_01204(atzE)

LBO:	LBWT_32140

HHG:	XM38_037730(atzE)

THEU:

HPC62_00925

GLP:	Glo7428_3273

MAR:

MAE_24190

MPK:

VL20_4002

MVZ:	myaer102_01190

HAO:	PCC7418_0381

ENN:	FRE64_16245

CYT:

cce_3546

CWA:	CwatDRAFT_1503

CYC:	PCC7424_2155

CYJ:	Cyan7822_1471

TER:

Tery_4203

ONI:	Osc7112_2828

MPRO:

BJP34_29850

MIC:	Mic7113_2411

NOS:	Nos7107_0892

NOP:	Nos7524_4531

NON:	NOS3756_01940

NFL:	COO91_06135

NOE:	CLI64_24855

NSH:

GXM_06212

NED:	HUN01_10365

ACY:	Anacy_4362

ANN:	EH233_21790

CSG:	Cylst_1741

CALO:

Cal7507_4595

CALT:

Cal6303_1204

CALH:

IJ00_16755

RIV:	Riv7116_5414

FIS:	FIS3754_34730

NSP:	BMF81_01350(atzE)

TOQ:	HCG51_22635

NCN:	BZZ01_26155

CTHE:

Chro_5195

PLP:	Ple7327_2132

SCS:	Sta7437_1016

STAN:

STA3757_18880

CAG:

Cagg_2536

TRO:

trd_0309

OBG:	Verru16b_02869(atzE)

VBH:	CMV30_14395

VBA:	IMCC26134_13690

SUS:

Acid_6116

» show all

Reference

1 [PMID:29523689]

Authors

Esquirol L, Peat TS, Wilding M, Liu JW, French NG, Hartley CJ, Onagi H, Nebl T, Easton CJ, Newman J, Scott C

Title

An unexpected vestigial protein complex reveals the evolutionary origins of an s-triazine catabolic enzyme.

Journal

J Biol Chem 293:7880-7891 (2018)
DOI:10.1074/jbc.RA118.001996

Sequence

[ag:AAK50332]

Other DBs

ExplorEnz - The Enzyme Database:

3.5.1.131

IUBMB Enzyme Nomenclature:

3.5.1.131

ExPASy - ENZYME nomenclature database:

3.5.1.131

BRENDA, the Enzyme Database:

3.5.1.131

DBGET integrated database retrieval system