KEGG ENZYME: 3.5.1.93

ENZYME: 3.5.1.93

Entry

EC 3.5.1.93                 Enzyme

Name

glutaryl-7-aminocephalosporanic-acid acylase;
7beta-(4-carboxybutanamido)cephalosporanic acid acylase;
cephalosporin C acylase;
glutaryl-7-ACA acylase;
CA;
GCA;
GA;
cephalosporin acylase;
glutaryl-7-aminocephalosporanic acid acylase;
GL-7-ACA acylase

Class

Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides

Sysname

(7R)-7-(4-carboxybutanamido)cephalosporanate amidohydrolase

Reaction(IUBMB)

(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O = (7R)-7-aminocephalosporanate + glutarate [RN:R07402]

Reaction(KEGG)

R07402

Substrate

(7R)-7-(4-carboxybutanamido)cephalosporanate [CPD:C15666];
H2O [CPD:C00001]

Product

(7R)-7-aminocephalosporanate [CPD:C07756];
glutarate [CPD:C00489]

Comment

Forms 7-aminocephalosporanic acid, a key intermediate in the synthesis of cephem antibiotics. It reacts only weakly with cephalosporin C.

History

EC 3.5.1.93 created 2005

Pathway

ec00311

Penicillin and cephalosporin biosynthesis

ec01110

Biosynthesis of secondary metabolites

Orthology

K12748

glutaryl-7-aminocephalosporanic-acid acylase

Reference

1 [PMID:7607251]

Authors

Ishii Y, Saito Y, Fujimura T, Sasaki H, Noguchi Y, Yamada H, Niwa M, Shimomura K.

Title

High-level production, chemical modification and site-directed mutagenesis of a cephalosporin C acylase from Pseudomonas strain N176.

Journal

Eur J Biochem 230:773-8 (1995)
DOI:10.1111/j.1432-1033.1995.0773h.x

Reference

2 [PMID:10739919]

Authors

Kinoshita T, Tada T, Saito Y, Ishii Y, Sato A, Murata M.

Title

Crystallization and preliminary X-ray analysis of cephalosporin C acylase from Pseudomonas sp. strain N176.

Journal

Acta Crystallogr D Biol Crystallogr 56 ( Pt 4):458-9 (2000)
DOI:10.1107/S0907444900000718

Reference

3 [PMID:10972935]

Authors

Monti D, Carrea G, Riva S, Baldaro E, Frare G.

Title

Characterization of an industrial biocatalyst: immobilized glutaryl-7-ACA acylase.

Journal

Biotechnol Bioeng 70:239-44 (2000)
DOI:10.1002/1097-0290(20001020)70:2<239::aid-bit13>3.0.co;2-i

Reference

4 [PMID:10945972]

Authors

Kwon TH, Rhee S, Lee YS, Park SS, Kim KH.

Title

Crystallization and preliminary X-Ray diffraction analysis of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas sp. GK16.

Journal

J Struct Biol 131:79-81 (2000)
DOI:10.1006/jsbi.2000.4256

Reference

5 [PMID:11080627]

Authors

Kim Y, Yoon K, Khang Y, Turley S, Hol WG

Title

The 2.0 A crystal structure of cephalosporin acylase.

Journal

Structure 8:1059-68 (2000)
DOI:10.1016/S0969-2126(00)00505-0

Sequence

[ag:AAF64242]

Reference

6 [PMID:11782466]

Authors

Huang X, Zeng R, Ding X, Mao X, Ding Y, Rao Z, Xie Y, Jiang W, Zhao G.

Title

Affinity alkylation of the Trp-B4 residue of the beta -subunit of the glutaryl 7-aminocephalosporanic acid acylase of Pseudomonas sp. 130.

Journal

J Biol Chem 277:10256-64 (2002)
DOI:10.1074/jbc.M108683200

Reference

7 [PMID:12680762]

Authors

Kim JK, Yang IS, Rhee S, Dauter Z, Lee YS, Park SS, Kim KH

Title

Crystal structures of glutaryl 7-aminocephalosporanic acid acylase: insight into autoproteolytic activation.

Journal

Biochemistry 42:4084-93 (2003)
DOI:10.1021/bi027181x

Other DBs

ExplorEnz - The Enzyme Database:

3.5.1.93

IUBMB Enzyme Nomenclature:

3.5.1.93

ExPASy - ENZYME nomenclature database:

3.5.1.93

BRENDA, the Enzyme Database:

3.5.1.93

CAS:	56645-46-6

DBGET integrated database retrieval system