KEGG ENZYME: 3.5.3.22

ENZYME: 3.5.3.22

Entry

EC 3.5.3.22                 Enzyme

Name

proclavaminate amidinohydrolase;
PAH;
proclavaminate amidino hydrolase

Class

Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amidines

Sysname

amidinoproclavaminate amidinohydrolase

Reaction(IUBMB)

amidinoproclavaminate + H2O = proclavaminate + urea [RN:R05357]

Reaction(KEGG)

R05357

Substrate

amidinoproclavaminate [CPD:C06657];
H2O [CPD:C00001]

Product

proclavaminate [CPD:C06658];
urea [CPD:C00086]

Comment

Forms part of the pathway for the biosythesis of the beta-lactamase inhibitor clavulanate in Streptomyces clavuligerus. It carries out an intermediary reaction between the first reaction of EC 1.14.11.21, clavaminate synthase, and the second and third reactions of that enzyme. Requires Mn2+.

History

EC 3.5.3.22 created 2003

Pathway

ec00331

Clavulanic acid biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K12676

proclavaminate amidinohydrolase

Genes

RAP:

RHOA_4422

APAU:

AMPC_01500(speB_2)

SANL:

KZO11_35500(speB)

SFA:

Sfla_0557

STRP:

F750_6322

SCLF:

BB341_07815 BB341_29475

SFK:

KY5_8016c

SKA:	CP970_43085(speB)

SVR:	CP971_33675(speB)

SSPB:

CP982_00550(speB)

SOV:	QZH56_31605(speB)

SFIY:

F0344_21140(speB)

KAB:	B7C62_18895

KAU:	B6264_26745

SVI:	Svir_33400

MSIL:

METEAL_21680(speB)

MSEA:

METESE_19060(speB)

» show all

Reference

1 [PMID:1998687]

Authors

Salowe SP, Krol WJ, Iwata-Reuyl D, Townsend CA.

Title

Elucidation of the order of oxidations and identification of an intermediate in the multistep clavaminate synthase reaction.

Journal

Biochemistry 30:2281-92 (1991)
DOI:10.1021/bi00222a034

Reference

2 [PMID:11472170]

Authors

Zhou J, Kelly WL, Bachmann BO, Gunsior M, Townsend CA, Solomon EI.

Title

Spectroscopic studies of substrate interactions with clavaminate synthase 2, a multifunctional alpha-KG-dependent non-heme iron enzyme: correlation with mechanisms and reactivities.

Journal

J Am Chem Soc 123:7388-98 (2001)
DOI:10.1021/ja004025+

Reference

3 [PMID:12413541]

Authors

Townsend CA.

Title

New reactions in clavulanic acid biosynthesis.

Journal

Curr Opin Chem Biol 6:583-9 (2002)
DOI:10.1016/S1367-5931(02)00392-7

Reference

4 [PMID:7601835]

Authors

Wu TK, Busby RW, Houston TA, McIlwaine DB, Egan LA, Townsend CA

Title

Identification, cloning, sequencing, and overexpression of the gene encoding proclavaminate amidino hydrolase and characterization of protein function in clavulanic acid biosynthesis.

Journal

J Bacteriol 177:3714-20 (1995)
DOI:10.1128/JB.177.13.3714-3720.1995

Other DBs

ExplorEnz - The Enzyme Database:

3.5.3.22

IUBMB Enzyme Nomenclature:

3.5.3.22

ExPASy - ENZYME nomenclature database:

3.5.3.22

BRENDA, the Enzyme Database:

3.5.3.22

DBGET integrated database retrieval system