KEGG ENZYME: 3.5.4.27

ENZYME: 3.5.4.27

Entry

EC 3.5.4.27                 Enzyme

Name

methenyltetrahydromethanopterin cyclohydrolase;
5,10-methenyltetrahydromethanopterin cyclohydrolase;
N5,N10-methenyltetrahydromethanopterin cyclohydrolase;
methenyl-H4MPT cyclohydrolase;
5,10-methenyltetrahydromethanopterin 10-hydrolase (decyclizing)

Class

Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In cyclic amidines

Sysname

5,10-methenyltetrahydromethanopterin 10-hydrolase (ring-opening)

Reaction(IUBMB)

5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O = 5-formyl-5,6,7,8-tetrahydromethanopterin [RN:R03464]

Reaction(KEGG)

R03464

Substrate

5,10-methenyl-5,6,7,8-tetrahydromethanopterin [CPD:C04330];
H2O [CPD:C00001]

Product

5-formyl-5,6,7,8-tetrahydromethanopterin [CPD:C01274]

Comment

Methanopterin is a pterin analogue. The enzyme is involved in the formation of methane from CO2 in Methanobacterium thermoautotrophicum.

History

EC 3.5.4.27 created 1989

Pathway

ec00680

Methane metabolism

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K01499

methenyltetrahydromethanopterin cyclohydrolase

Genes

MCA:

MCA2863

METU:

GNH96_03530(mch)

MMEO:

OOT43_14015(mch)

MMT:	Metme_2265

MDN:	JT25_019335

MDH:	AYM39_08540

MKO:	MKLM6_1778

METL:

U737_14820

MPAD:

KEF85_11475

MELL:

IVG45_03930

MRP:	NM686_014190(mch)

MMOT:

QZJ86_13655(mch)

MAH:	MEALZ_2425(mch)

MBUR:

EQU24_09125

MPSY:

CEK71_15610

MMAI:

sS8_3394

MSZE:

MSZNOR_2917(mch)

MMOB:

F6R98_19020

MEIN:

methR_P2328

MOZ:	MoryE10_28870(mch)

MISZ:

MishRS11D_40060(mch)

MESL:

KKZ03_09080(mch)

MEIY:

MIN45_P1841

MCAU:

MIT9_P1441

MEJ:

Q7A_44

MEC:

Q7C_1772

MPIN:

LGT42_002855(mch)

MMAF:

GCM100_17930(mch)

MTHA:

VSX76_15975(mch)

BLEP:

AL038_01000

NHL:

Nhal_3876

NWR:	E3U44_04675

TSY:	THSYN_31780

SEDS:

AAY24_01650

COX:	E0W60_31930

BUG:	BC1001_3948

BGF:	BC1003_5455

BYI:	BYI23_B014460

BUE:	BRPE67_BCDS01450

BUQ:	AC233_22025

BXE:	Bxe_B2433(mch)

BXB:	DR64_4761(mch)

BPH:	Bphy_5946 Bphy_6484

BGE:	BC1002_3223

BPX:	BUPH_00532

BCAI:

K788_0001556

PSPW:

BJG93_19395(mch)

PARA:

BTO02_24125

PARB:

CJU94_31195

PHS:	C2L64_21815 C2L64_38295

PTER:

C2L65_26155 C2L65_34290

PGP:	CUJ91_21500

PCJ:	CUJ87_23420

PTS:	CUJ90_28070

PGIS:

I6I06_22465

PEW:	KZJ38_25875(mch)

PDIO:

PDMSB3_0629.1(mch)

PSAA:

QEN71_32820(mch) QEN71_34085(mch)

PKF:	RW095_18805(mch)

PPAI:

E1956_20450

BUO:	BRPE64_BCDS01590

CABK:

NK8_41330

TCAR:

U0034_17530(mch)

VAP:	Vapar_3082

VPD:	VAPA_1c31460(mch)

MPT:

Mpe_A2609

METP:

C1M51_15095

RGE:	RGE_40250(mch)

ATER:

MW290_07945(mch)

LCH:

Lcho_3150

AON:	DEH84_18995

METR:

BSY238_3292(mch)

MFA:

Mfla_1658

MMB:

Mmol_1344

MEH:

M301_1554

MEI:	Msip34_1502

MEP:

MPQ_1565

MBAC:

BN1209_0747(mch)

MEU:	ACJ67_08170

MPAU:

ZMTM_11840(mch)

SPLB:

SFPGR_25980(mch)

AZA:	AZKH_p0273

AZD:	CDA09_11315

THU:	AC731_004845

THAU:

C4PIVTH_3698(mch)

THK:	CCZ27_13250

DDZ:	DSYM_25690

MCI:	Mesci_4260

MCIC:

A4R28_05755

MESW:

A9K65_023620

MOH:	IHQ72_13875

MMEI:

LRP31_14005(mch)

PHT:	BLM14_23790

RGA:	RGR602_PC01568(mch)

NPM:	QEO92_19220(mch)

XAU:

Xaut_1788

XDI:	EZH22_07500

STAR:

G3545_09780

LNE:	FZC33_09335

SNO:

Snov_0750

ANC:	GBB76_00750

APRA:

G3A50_16495

APOL:

K9D25_20190(mch)

MEA:	Mex_1p1763(mch)

MDI:	METDI2515(mch)

MEX:

Mext_1831

MCH:

Mchl_2166

MPO:

Mpop_1782

MZA:	B2G69_16815

MRD:	Mrad2831_0530

MET:

M446_5780

MNO:

Mnod_6004

MOR:	MOC_0685(mch)

META:

Y590_08770

MAQU:

Maq22A_c16475(mch)

MPHY:

MCBMB27_00107

MEE:	DA075_05730

METD:

C0214_10525

METX:

A3862_28300

METS:

DK389_20655

METI:

DK427_18445

MMES:

MMSR116_16690

MTEA:

DK419_10795

MIND:

mvi_45910(mch)

MOG:	MMB17_20025(mch)

MTAD:

M6G65_13665(mch)

MSL:

Msil_2387

MTUN:

MTUNDRAET4_2723(mch)

MLG:	CWB41_06045

MEDK:

QEV83_11770(mch)

HDN:

Hden_1477

HDT:	HYPDE_31258

HMC:	HYPMC_1627(mch)

HNI:	W911_13175

FIL:	BN1229_v1_0586(mch)

FIY:	BN1229_v1_0588(mch)

MCG:

GL4_1786

METG:

HT051_10180

MSC:

BN69_1353

MBRY:

B1812_02320

MROS:

EHO51_13055

MHEY:

H2LOC_003815

MPAR:

F7D14_05050

MIWA:

SS37A_00070(mch_1)

MTW:	CQW49_13495

MECQ:

MSC49_12090(mch)

CMET:

K6K41_25475(mch)

PLEO:

OHA_1_01035(mch)

HDI:	HDIA_2312(mch)

AALA:

IGS74_05780

AALM:

LUX29_06535(mch)

AUZ:	Sa4125_38530(mch)

AUB:	LXB15_00980(mch)

JAV:	OXU80_18200(mch)

GBE:	GbCGDNIH1_0773

GBH:	GbCGDNIH2_0773

GBC:	GbCGDNIH3_0773

GBS:	GbCGDNIH4_0773

ALI:	AZOLI_p10687(mch1) AZOLI_p10889(mch2)

ABS:	AZOBR_p270082(mch)

ABQ:	ABAZ39_25470

ABF:	AMK58_22330

ATI:	AL072_27075

AZT:	TSH58p_01205

AZM:	DM194_17080

AOZ:	HUE56_08920

AARE:

D3093_24960

AZS:	E6C72_18245 E6C72_22455

CBT:	CLH_1638(mch)

PALM:

RBG61_02415(mch)

CMIC:

caldi_15520(mch)

DMT:	DESME_10945

DOR:	Desor_4012

DAI:	Desaci_3581

DMI:	Desmer_3120

FWA:	DCMF_01135 DCMF_03915

CBOL:

CGC65_05105

HOR:	Hore_19220

HAS:	Halsa_0947

MPHE:

HGG69_02450

CAP:	CLDAP_00590(mch)

RBA:	RB6759(mch)

PSL:

Psta_3184

PIR:	VN12_11975(mch)

RUL:	UC8_55600(mch)

RML:	FF011L_43370(mch)

MFF:	MFFC18_17580(mch)

ROL:	CA51_33360(mch)

RUV:	EC9_35480(mch)

AHEL:

Q31a_62590(mch)

LCRE:

Pla8534_46610(mch)

AAGG:

ETAA8_05120(mch)

BVO:	Pan97_33090(mch)

BREM:

PSR63_21930(mch)

RLC:	K227x_27840(mch)

SMAM:

Mal15_33980(mch)

SNEP:

Enr13x_45510(mch)

LPAV:

PLANPX_1003

LLH:	I41_11730(mch)

AMUC:

Pan181_44380(mch)

PND:	Pla175_40180(mch)

AMOB:

HG15A2_09750(mch)

PLM:

Plim_2586

PEH:	Spb1_33570(mch)

PBS:	Plabr_2150

PLS:	VT03_28970(mch)

PLH:	VT85_05465(mch)

FMR:	Fuma_00366(mch)

GMR:	GmarT_12110(mch)

GIM:	F1728_08720

GPN:	Pan110_11880(mch)

GFM:	Enr17x_12910(mch)

MRI:	Mal4_29800(mch)

SDYN:

Mal52_18780(mch)

PLON:

Pla110_19000(mch)

ACAF:

CA12_34930(mch)

SVP:	Pan189_27040(mch)

TPOL:

Mal48_18260(mch)

CHYA:

V22_26960(mch)

CCOS:

Pan44_15750(mch)

PVAR:

SH412_003487(mch)

CCOP:

Mal65_23800(mch)

GES:	VT84_09780(mch)

GOG:	C1280_06325(mch)

GMS:	SOIL9_11460

TIM:	GMBLW1_05840

LRS:	PX52LOC_02773(mch)

FTJ:

FTUN_8522

ULI:	ETAA1_18410(mch)

TSPH:

KIH39_22355(mch)

IPA:

Isop_0325

SACI:

Sinac_1236

PBOR:

BSF38_00163(mch)

AGV:	OJF2_59280(mch)

SSM:	Spirs_2846

AMO:	Anamo_1135

ARAM:

KAR29_10130

MOX:	DAMO_0461(mch)

MJA:

MJ_1636

MFE:	Mefer_1327

MVU:	Metvu_1361

MFS:	MFS40622_0639

MIF:	Metin_0908

MJH:	JH146_0538

MESG:

MLAUSG7_0104(mch)

MESA:

MLASG1_1287(mch)

MIG:	Metig_0087

MMP:	MMP1191(mch)

MMQ:	MmarC5_0399

MMX:	MmarC6_1482

MMZ:	MmarC7_0438

MMD:

GYY_06780

MMAK:

MMKA1_13660(mch)

MMAO:

MMOS7_12990(mch)

MMAD:

MMJJ_16540(mch)

MAE:

Maeo_0441

MVN:	Mevan_0507

MVO:

Mvol_0378

MOK:	Metok_1590

METF:

CFE53_06735(mch)

MTH:

MTH_773

MMG:	MTBMA_c11690(mch)

MWO:	MWSIV6_1147(mch)

MTEE:

MTTB_06150

METC:

MTCT_0692

METE:

tca_00740(mch)

METZ:

METMT2_0406

METK:

FVF72_07680(mch)

MTHM:

FZP57_05450(mch)

METJ:

FZP68_00760(mch)

MST:	Msp_1238(mch)

METB:

AW729_09545

MEIS:

PXD04_01400(mch)

MRU:	mru_1619(mch)

MSI:

Msm_1723

MEB:	Abm4_1203(mch)

MMIL:

sm9_1330(mch)

MEYE:

TL18_04360

MOL:

YLM1_1103

MARO:

MarbSA_04250

METV:

K4897_00150(mch)

MEL:	Metbo_2195

MEW:	MSWAN_0451

METH:

MBMB1_0537(mch)

MFC:	BRM9_1751(mch)

MFI:	DSM1535_2390(mch)

MCUB:

MCBB_0462(mch)

MSUB:

BK009_00605

METN:

BK008_08410

METT:

CIT01_01545(mch)

METO:

CIT02_04240(mch)

MEME:

HYG87_03185(mch)

MFEG:

GCM10025860_14460

MFV:

Mfer_0254

MKA:	MK0625(mch)

AFU:

AF_1935

AFG:	AFULGI_00021950

APO:	Arcpr_0990

AVE:	Arcve_2011

AST:	Asulf_01966

FPL:

Ferp_0606

GAC:

GACE_0343

GAH:

GAH_01155

MBA:	Mbar_A2233

MBY:	MSBRM_1678

MBW:	MSBRW_2514

MBAR:

MSBR2_0727

MBAK:

MSBR3_0457

MAC:	MA_1710(mch)

MMA:

MM_2653

MMAZ:

MmTuc01_2718

MMJ:	MSMAS_2053

MMAC:

MSMAC_1059

MVC:	MSVAZ_1281

MEK:	MSKOL_1225

MLS:	MSLAZ_1037

METM:

MSMTP_2066

MEF:	MSWH1_1963

MEQ:	MSWHS_3107

MSJ:	MSSAC_3299

MSZ:	MSSIH_1132

MSW:	MSSIT_1182

MTHR:

MSTHT_0435

MTHE:

MSTHC_0286

MHOR:

MSHOH_2598

MFZ:	AOB57_000035(mch)

MBU:	Mbur_0653(mch)

MMET:

MCMEM_0808

MELO:

J7W08_02205(mch)

MMH:

Mmah_1138

MHAZ:

BHR79_07850

MPOT:

BKM01_08510

MEV:	Metev_1611

MZH:	Mzhil_1348

MPY:

Mpsy_3012

MZI:	HWN40_06075(mch)

MMAV:

RE476_04520(mch)

MSEB:

RE474_00635(mch)

MHZ:	Metho_1529

MTP:

Mthe_0252

MCJ:	MCON_1290(mch)

MHI:

Mhar_2174

MHU:	Mhun_0444 Mhun_2384

MRTJ:

KHC33_12350(mch) KHC33_16595(mch)

MLA:

Mlab_0399

MEM:	Memar_0263 Memar_0668

MBG:	BN140_0141(mch1) BN140_1778(mch3)

MEMA:

MMAB1_2292(mch)

MCHK:

MchiMG62_07930

MSUM:

OH143_08070(mch) OH143_10070(mch)

MRC:	R6Y96_02265(mch) R6Y96_09325(mch)

MPI:

Mpet_0065

MEND:

L6E24_01800(mch) L6E24_07730(mch)

MEFW:

F1737_10680(mch)

MAQE:

RJ40_10830(mch)

MFK:	E2N92_11075(mch)

MOU:	OU421_09265(mch)

MESB:

L1S32_00440(mch)

MANQ:

L1994_10950(mch)

MBN:

Mboo_0680

MFO:	Metfor_2532

MPL:

Mpal_2022

MPD:	MCP_1132(mch-1) MCP_1888(mch-2)

MEZ:	Mtc_1063(mch-1) Mtc_1978(mch-2)

RCI:	LRC191(mch-1) RCIX2742(mch-2)

HAL:	VNG_1686G(mch)

HSL:	OE_3383R(mch)

HANR:

LJ422_06805(mch)

HDL:	HALDL1_13235

HHB:	Hhub_3179(mch)

HAGS:

JT689_08135

HABO:

JRZ79_06550(mch)

HNO:	LT974_11800(mch)

HLU:	LT972_03110(mch)

HALH:

HTSR_0323

HHSR:

HSR6_0309(mch)

HSU:	HLASF_1805

HSF:	HLASA_1791

HAHS:

HSRCO_2408(mch3)

SALR:

FQU85_07455

HALR:

EFA46_001470

HJE:	HacjB3_04520

HDO:	MUK72_08695(mch)

HALX:

M0R89_02735(mch)

HAXZ:

M0R88_02720(mch)

HAYC:

NGM10_12675(mch)

HAXY:

NGM07_07235(mch)

SALI:

L593_12155

SAIM:

K0C01_07840(mch)

HARA:

AArcS_1105(mch2)

HMA:	rrnAC1616(mch)

HHI:	HAH_2159(mch)

HHN:	HISP_11005

HAB:	SG26_03375

HTA:	BVU17_10515

HALJ:

G9465_15375

HSIN:

KDQ40_02600

NPH:	NP_2634A(mch)

NMO:	Nmlp_2018(mch)

NHO:	HWV23_16790

NSAL:

HWV07_18180

HUT:

Huta_1145

HTI:

HTIA_1038

HALA:

Hrd1104_00960

HASV:

SVXHr_0469(mch)

HABN:

HBNXHr_0448(mch)

HMU:

Hmuk_1816

HALZ:

E5139_12200

HALL:

LC1Hm_1117(mch)

HAZP:

GBQ70_12210

HALI:

BV210_13610

HSN:	DV733_14155

HDS:	HSR122_2710(mch)

HRR:	HZS55_12710

HPEL:

HZS54_16640

HLT:	I7X12_01230

HARC:

HARCEL1_01570

SSAI:

N0B31_10465(mch)

HAAD:

MW046_11205(mch)

HWA:	HQ_2798A(mch)

HWC:	Hqrw_3170(mch)

HVO:

HVO_2573

HME:

HFX_2599

HGI:	ABY42_12905

HALE:

G3A49_16560

HLS:	KU306_00075(mch)

HLN:	SVXHx_2025(mch2)

HBO:	Hbor_10140

HAQ:	DU484_04960

HAJ:	DU500_05400

HAER:

DU502_03105

HRA:	EI982_10145

HLM:	DV707_04070

HALM:

FCF25_02980

HLA:

Hlac_1693

HALP:

DOS48_08545

HALB:

EKH57_03305

HEZZ:

EO776_08115

HALQ:

Hrr1229_003010

HSS:	J7656_08315

HSAI:

HPS36_13725

HAZZ:

KI388_05770(mch)

SRUB:

C2R22_15900

HAE:	halTADL_3392

HALN:

B4589_001465

HALG:

HUG10_00565

HALU:

HUG12_17145

HMP:	K6T50_14670(mch)

HRE:	K6T36_14630(mch)

HRM:	K6T25_02130(mch)

HACB:

Hbl1158_06735(mch)

HDF:	AArcSl_1968(mch)

HAH:	Halar_1179

HTU:

Htur_2210

HSAL:

JMJ58_17890

HAKZ:

J0X25_17525(mch)

NMG:	Nmag_0205(mch)

HXA:	Halxa_1038

NAT:

NJ7G_2078

NPE:	Natpe_0864

NVR:	FEJ81_04450

NPL:	FGF80_06355

NAY:	HYG81_05585(mch)

HALY:

HYG82_07980

HJT:	DVR14_13650

HLO:	J0X27_04755

NGE:	Natgr_2851

HRU:	Halru_2071

SAWL:

NGM29_13965(mch)

HALV:

NGM15_05240(mch)

NOU:	Natoc_3488

HLR:	HALLA_14455

HLC:	CHINAEXTREME13965

NAJ:	B1756_01145

NAG:	AArcMg_2172

NAN:	AArc1_1514

NARA:

QQ977_01390(mch)

NBG:	DV706_00340 DV706_16930

NAS:	GCU68_16020

HDA:	BB347_06500

MELU:

MTLP_03080

MEAM:

MU439_06355(mch)

MEAE:

QEN48_03790(mch)

BARC:

AOA65_2050(mch)

LOKI:

Lokiarch_00580(mch_1) Lokiarch_17800(mch_2)

» show all

Reference

1 [PMID:4062290]

Authors

Donnelly MI, Escalante-Semerena JC, Rinehart KL Jr, Wolfe RS.

Title

Methenyl-tetrahydromethanopterin cyclohydrolase in cell extracts of Methanobacterium.

Journal

Arch Biochem Biophys 242:430-9 (1985)
DOI:10.1016/0003-9861(85)90227-9

Other DBs

ExplorEnz - The Enzyme Database:

3.5.4.27

IUBMB Enzyme Nomenclature:

3.5.4.27

ExPASy - ENZYME nomenclature database:

3.5.4.27

UM-BBD (Biocatalysis/Biodegradation Database):

3.5.4.27

BRENDA, the Enzyme Database:

3.5.4.27

CAS:	99533-50-3

DBGET integrated database retrieval system